Pediatric reference data on activity of urinary N-acetyl-β-D-hexosaminidase and its isoenzymes

Lysosomal exoglycosidases in the urine of children and adolescents

“…Having classified children into 6 age groups with 3-year time intervals, we showed statistically significant differences (p < 0.05) in the urinary activity of HEX and its isomers (pKat/µg Cr) between the youngest children (0.5-2.9 years) and adolescents (15.0-17.9 years). An inverse correlation was found between age and urinary activity of HEX and its isoenzymes when expressed as creatinine: HEX: r = −0.24, p < 0.001; HEX A: r = −0.20, p < 0.01 and HEX B: r = −0.26, p < 0.001 (42) . The obtained findings partly correspond to those presented by Agirbasli et al (43) , who demonstrated that urinary activity of HEX in young people (18-32 years) depends on age, sex, physical exercise, and blood pressure.…”

“…Furthermore, we showed a negative correlation between the age of children and adolescents and urinary activity (pKat/µg Cr) of FUC (r = −0.36, p < 0.0001); GAL (r = −0.36, p < 0.0001); GLU (r = −0.35, p < 0.0001) and MAN (r = −0.35, p < 0.0001) (46) . Our study is the first such a large analysis on reference values for urine activity of HEX, its isoenzymes HEX A and HEX B as well as FUC, GAL, GLU and MAN in healthy children and adolescents (42,46) . The relationship between urinary activity of lysosomal exoglycosidases in children/adolescents and age and the percentile chart within normal values of the 3 rd , 10 th , 25 th , 50 th , 75 th , 90 th and 97 th percentile may be of practical importance for differentiating between healthy and unhealthy children and adolescents (42,46) .…”

“…Therefore, we decided to assess changes in urinary activity of HEX and its isoenzymes, i.e. HEX A and HEX B, in a group of 230 children and adolescents (42) . Having classified children into 6 age groups with 3-year time intervals, we showed statistically significant differences (p < 0.05) in the urinary activity of HEX and its isomers (pKat/µg Cr) between the youngest children (0.5-2.9 years) and adolescents (15.0-17.9 years).…”

Reference Data on Neonatal Serum N-Acetyl-β-hexosaminidase Activity

“…Our results are compatible with the report of Agirbasli et al [ 32 ], who proved that urinary HEX excretion by young people (18–32 years old) was dependent on age, gender, race, and blood pressure. Recently Zalewska-Szajda et al [ 33 ] also reported that reference activities of urinary HEX in children and adolescents are significantly dependent on age but not gender.…”