First Complete Genome Sequence of
            <i>Potato leafroll virus</i>
            from Argentina

Barón, María Pilar Barrios; Agrofoglio, Yamila Carla; Delfosse, Verónica Cecilia; Nahirñak, Vanesa; Urreta, Martín Salvador González de; Almasia, Natalia Inés; Rovere, Cecilia Vázquez; Distéfano, Ana Julia

doi:10.1128/genomea.00628-17

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…The genomes of the members contain 4 to 10 open reading frames (ORFs) with a positive-sense RNA molecule of 4 kb to 6 kb without a 3 -poly(A) tail [25]. The family includes important plant pathogens with high economic impact, with rice yellow mottle virus, potato leafroll virus and sugarcane yellow leaf virus (ScYLV) being some of the most devastating [26][27][28]. Here, we identified two novel solemoviruses: Guiyang Paspalum distichum solemo-like virus 1 (GPDV1) and Xiaogan Aeschynomene indica solemo-like virus 1 (XAISV1).…”

Section: Characterization Of Postive-sense Rna Virusesmentioning

confidence: 99%

Novel RNA Viruses Discovered in Weeds in Rice Fields

Chao

Wang

Zhang

et al. 2022

Viruses

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Weeds often grow alongside crop plants. In addition to competing with crops for nutrients, water and space, weeds host insect vectors or act as reservoirs for viral diversity. However, little is known about viruses infecting rice weeds. In this work, we used metatranscriptomic deep sequencing to identify RNA viruses from 29 weed samples representing 23 weed species. A total of 224 RNA viruses were identified: 39 newly identified viruses are sufficiently divergent to comprise new families and genera. The newly identified RNA viruses clustered within 18 viral families. Of the identified viruses, 196 are positive-sense single-stranded RNA viruses, 24 are negative-sense single-stranded RNA viruses and 4 are double-stranded RNA viruses. We found that some novel RNA viruses clustered within the families or genera of several plant virus species and have the potential to infect plants. Collectively, these results expand our understanding of viral diversity in rice weeds. Our work will contribute to developing effective strategies with which to manage the spread and epidemiology of plant viruses.

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Section: Characterization Of Postive-sense Rna Virusesmentioning

confidence: 99%

Novel RNA Viruses Discovered in Weeds in Rice Fields

Chao

Wang

Zhang

et al. 2022

Viruses

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“…Potato leafroll Polerovirus (PLRV), from the genus Polerovirus and the family Solemoviridae, consists of a 5.3-5.7 kb long positive sense (+) monopartite singlestranded RNA [(+)ssRNA] genome with virus protein genomelinked (VPg) cap bounded at the 5′ end and an OH group at the 3′ end without poly(A) tail or tRNA-like organization Walker et al, 2021). Typically, the PLRV genome contains 6-7 overlapping open reading frames (ORFs), which are organized into genomic and sub-genomic RNAs (Figure 1; Krueger et al, 2013;Sõmera et al, 2015;Barrios Barón et al, 2017). The RNA-dependent RNA polymerase (RdRp), expressed via ribosomal frameshifting, is a translational fusion of ORF1 that encodes P1 and ORF2, which encodes P2.…”

Section: Introductionmentioning

confidence: 99%

Global genetic diversity and evolutionary patterns among Potato leafroll virus populations

Farooq

Hussain²,

Shakeel³

et al. 2022

Front. Microbiol.

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Potato leafroll virus (PLRV) is a widespread and one of the most damaging viral pathogens causing significant quantitative and qualitative losses in potato worldwide. The current knowledge of the geographical distribution, standing genetic diversity and the evolutionary patterns existing among global PLRV populations is limited. Here, we employed several bioinformatics tools and comprehensively analyzed the diversity, genomic variability, and the dynamics of key evolutionary factors governing the global spread of this viral pathogen. To date, a total of 84 full-genomic sequences of PLRV isolates have been reported from 22 countries with most genomes documented from Kenya. Among all PLRV-encoded major proteins, RTD and P0 displayed the highest level of nucleotide variability. The highest percentage of mutations were associated with RTD (38.81%) and P1 (31.66%) in the coding sequences. We detected a total of 10 significantly supported recombination events while the most frequently detected ones were associated with PLRV genome sequences reported from Kenya. Notably, the distribution patterns of recombination breakpoints across different genomic regions of PLRV isolates remained variable. Further analysis revealed that with exception of a few positively selected codons, a major part of the PLRV genome is evolving under strong purifying selection. Protein disorder prediction analysis revealed that CP-RTD had the highest percentage (48%) of disordered amino acids and the majority (27%) of disordered residues were positioned at the C-terminus. These findings will extend our current knowledge of the PLRV geographical prevalence, genetic diversity, and evolutionary factors that are presumably shaping the global spread and successful adaptation of PLRV as a destructive potato pathogen to geographically isolated regions of the world.

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“…In particular, the green peach aphid, Myzus persicae, is the most efficient and important PLRV vector (Mondal et al, 2017). A reported isolate of Argentinian PLRV has a monopartite positive-sense RNA genome of about 5.9 kb with 10 open reading frames (ORFs) (Barrios Barón et al, 2017). This virus is distributed within all major potato-growing regions of Argentina affecting yield and tuber quality, and causes one of the most important potato diseases causing losses of up to 90% of the crop (https://www.sinav imo.gov.ar/plaga/ potat o-leafr oll-virus).…”

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confidence: 99%

Argentinian potato leafroll virus P0 protein: Novel activities for a previously known suppressor

et al. 2020

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The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL‐Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL‐Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL‐Ar impedes accumulation of dsRNA‐derived siRNAs. We also showed that P0PL‐Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F‐box‐like domain. A mutant in the GW/WG motif within the P0PL‐Ar F‐box‐like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL‐Ar F‐box‐like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL‐Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer‐like enzyme (DCL)‐mediated primary siRNA production and downstream at the RNA‐induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors.

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First Complete Genome Sequence of Potato leafroll virus from Argentina

Cited by 3 publications

References 2 publications

Novel RNA Viruses Discovered in Weeds in Rice Fields

Novel RNA Viruses Discovered in Weeds in Rice Fields

Global genetic diversity and evolutionary patterns among Potato leafroll virus populations

Argentinian potato leafroll virus P0 protein: Novel activities for a previously known suppressor

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