In vitro antifungal activity of Myracrodruon urundeuva Allemão against human vaginal Candida species

Abstract

Myracrodruon urundeuva is a plant native to Brazil, which is used by the indigenous population for the treatment of candidiasis. The aims of this study were to evaluate the antifungal activity of extract against human vaginal Candida species and evaluate the possible toxicological activities of M. urundeuva. Initially, ethanol extracts, ethyl acetate fractions, and hydroalcoholic fractions of the bark and leaf of M. urundeuva were used to determine the minimum inhibitory concentration. The extracts that showed antifungal activity were characterized by liquid chromatography and subjected to toxicity assessment. Toxic, cytotoxic, genotoxic, and mutagenic testing were performed using Allium cepa and Ames assays with the ethanol extracts of the bark and leaves. Hemolytic activity was evaluated in erythrocytes and acute toxicity in rats. The ethanol bark extracts showed best activity against Candida albicans, C. krusei, and C. tropicalis ATCC (4-512 µg/mL). Chemical characterization indicated the presence of flavonoids and tannins in the extracts. Hemolytic activity, genotoxicity, and mutagenicity were not observed. The results of the Ames and A. cepa tests were also in agreement, ethanol bark extracts and ethanol leaf extracts of M. urundeuva showed absence of mutagenic activity. Similar results were observed in the A. cepa assay and acute toxicity test in rats. M. urundeuva bark extracts showed potential for the treatment of vaginal infections caused Candida species, as a topical.

Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata

“…Furthermore, the antifungal activity was retained when the extract was incorporated into a nanostructured lipid system (Bonifácio et al, 2015). Other groups have also reported on the antifungal activity of A. urundeuva bark and leaves against different species of Candida (Oliveira et al, 2017). Thus, in an initial set of experiments we used CLSI techniques to assess the antifungal activity of both the free extract and the extract incorporated in an improved microemulsion formulation against two other collection strains of C. albicans , and also expanded these studies to a C. glabrata collection strain (Table 1).…”

Genotoxic Assessment of the Dry Decoction of Myracrodruon urundeuva Allemão (Anacardiaceae) Leaves in Somatic Cells of Drosophila melanogaster by the Comet and SMART Assays

“…Many reports on the pharmacological properties of extracts and isolated compounds from M. urundeuva are found in the literature, corroborating its uses as antiulcer and gastroprotective (Carlini et al ), anti‐inflammatory and analgesic (Souza et al ), and antimicrobial against bacteria, fungi, and viruses (Sá et al ; Figueredo et al ; Cecilio et al ; Oliveira et al ). However, despite its large use in traditional herbal medicine, only a few reports on its toxicological effect can be found in the literature.…”

Educação fitoterápica e ambiental como meio de propagação do seu uso racional através da extensão universitária para a comunidade: relato de experiência

“…A planta Myracrodruon urundeuva Allemão conhecida como "Aroeira do sertão" e encontrada em abundância no Brasil (Botelho et al, 2007) se destaca na medicina popular e na farmacologia em razão de suas propriedades anti-inflamatórias, cicatrizantes, gastroprotetoras, antiúlceras, analgésicas e antifúngicas (Viana et al, 2003;Souza et al, 2007;Oliveira et al, 2017;Galvão et al, 2018).…”