Hydroxycinnamoyl derivatives and secoiridoid glycoside derivatives from Syringa vulgaris flowers and their effects on the pro-inflammatory responses of human neutrophils

UHPLC‐DAD‐ESI‐MS/MS and HPTLC profiling of ash leaf samples from different commercial and natural sources and their in vitro effects on mediators of inflammation

“…Fragmentation of this ion gave an ion at m/z 209 due to coumaroyl cleavage (loss of 146 amu), indicating that the compounds were coumaroyl hexaric acids. Such derivatives were identified in Syringa vulgaris and Ligustrum vulgare L. (Oleaceae) . Notably, for the first time, hydroxycinnamoyl hexaric acids were observed in Fraxinus excelsior .…”

“…Compound 59 ( t r = 39.9 min) exhibited a pseudomolecular ion [M‐H] − at m/z 1009 and fragment ions at m/z 847 [M‐H‐162] − , m/z 623 [M‐H‐162‐224] − and m/z 461 [M‐H‐162‐224‐162] − , corresponding to the cleavage of hexose, elenolic acid and caffeoyl moieties, respectively. The compound was then identified by comparison with the reference standard as oleoacteoside, which was previously identified in Syringa sp …”

“…Quercetin‐3‐ O ‐rutinoside was purchased from Roth (Karlsruhe, Germany). Oleuropein and verbascoside/acteoside were isolated as described previously . All solvents used for chromatography were of gradient grade.…”

“…UV spectra were recorded in the range 200–400 nm. Compounds were identified by comparing their retention time and UV‐visible and mass spectra with those obtained from standard compounds isolated previously from other Oleaceae family members and/or tentatively identified by comparison with literature information.…”

“…Peripheral venous blood was taken from healthy human donors (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) years old) at the Warsaw Blood Donation Centre. The donors did not smoke or take any medications and were clinically recognised to be healthy.…”