Mapping the Glyco-Gold Nanoparticles of Different Shapes Toxicity, Biodistribution and Sequestration in Adult Zebrafish

Sangabathuni, Sivakoti; Murthy, Raghavendra Vasudeva; Chaudhary, Preeti; Subramani, Balamurugan; Toraskar, Suraj; Kikkeri, Raghavendra

doi:10.1038/s41598-017-03350-3

Cited by 46 publications

(30 citation statements)

References 39 publications

(27 reference statements)

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“…This same tendency was found for the endpoint of biodistribution, as nanorods distributed throughout tumor tissues, whereas gold spheres and discs were located only at the surface of the tumor (Black et al 2014). In addition, nanorods showed the fastest uptake and clearance over spherical and star-shaped NPs (Sangabathuni et al 2017). Moreover, the aspect ratio of rods was found to determine uptake and internal distribution: short rods were taken up faster and were trapped in the liver, while longer rods with a smaller aspect ratio showed lower uptake efficiency and were trapped in the spleen of mice (Qiu et al 2010;Huang et al 2011;Truong et al 2015).…”

Section: Introductionsupporting

confidence: 71%

The biodistribution and immuno-responses of differently shaped non-modified gold particles in zebrafish embryos

et al. 2019

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Important questions raised in (nano)ecotoxicology are whether biodistribution of nanoparticles (NPs) is affected by particle shape and to what extent local adverse responses are subsequently initiated. For nanomedicine, these same questions become important when the labeled NPs lose the labeling. In this study, we investigated the biodistribution patterns of gold nanoparticles (AuNPs) as well as immune-related local and systemic sublethal markers of exposure and behavioral assessment. Hatched zebrafish embryos were exposed to four differently shaped non-coated AuNPs with comparable sizes: nanospheres, nanorods, nano-urchins, and nanobipyramids. Shape-dependent trafficking of the particles resulted in a different distribution of the particles over the target organs. The differences across the distribution patterns indicate that the particles behave slightly different, although they eventually reach the same target organsyet in different ratios. Mainly local induction of the immune system was observed, whereas systemic immune responses were not clearly visible. Macrophages were found to take AuNPs from the body fluid, be transferred into the veins and transported to digestive organs for clearance. No significant behavioral toxicological responses in zebrafish embryos were observed after exposure. The trafficking of the particles in the macrophages indicates that the particles are removed via the mononuclear phagocytic system. The different ratios in which the particles are distributed over the target organs indicate that the shape influences their behavior and eventually possibly the toxicity of the particles. The observed shape-dependent biodistribution patterns might be beneficial for shape-specific targeting in nanomedicine and stress the importance of incorporating shape-features in nanosafety assessment.

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Section: Introductionsupporting

confidence: 71%

The biodistribution and immuno-responses of differently shaped non-modified gold particles in zebrafish embryos

et al. 2019

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“…Shape represents another important factor mediating the effects of Au NPs. The toxicity and biodistribution of fluorescently-labeled Au NPs of different shapes were examined in adult zebrafish [ 91 ]. Rod-shaped Au NPs exhibited enhanced uptake and clearance, while star-shaped Au NPs in contrast displayed slower uptake but longer sequestration in comparison to rod-shaped or spherical particles.…”

Section: Selected Nanotoxicology Studies In Zebrafishmentioning

confidence: 99%

Zebrafish as a Model to Evaluate Nanoparticle Toxicity

2018

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Nanoparticles are increasingly being developed for in vivo use, from targeted drug delivery to diagnostics, where they have enormous potential, while they are also being used for a variety of applications that can result in environmental exposure for humans. Understanding how specific nanoparticles interact with cells and cell systems is essential to gauge their safety with respect to either clinical or environmental exposure. Zebrafish is being increasingly employed as a model to evaluate nanoparticle biocompatibility. This review describes this model and how it can be used to assess nanoparticle toxicity at multiple levels, including mortality, teratogenicity, immunotoxicity, genotoxicity, as well as alterations in reproduction, behavior and a range of other physiological readouts. This review also provides an overview of studies using this model to assess the toxicity of metal, metal oxide and carbon-based nanoparticles. It is anticipated that this information will inform research aimed at developing biocompatible nanoparticles for a range of uses.

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“…The developing zebrafish is an established model allowing the identification of toxicity mechanisms, and thus the assessment in vivo GNP toxicity . Zebrafish embryos were exposed to 1.3 nm GNP with nonrenewal doses from 0.008 to 5 μg.…”

Section: Toxicity and Distribution Of Gold Nanoparticles In The Eyementioning

confidence: 99%

Gold nanoparticles in ophthalmology

Masse

Ouellette

Lamoureux

et al. 2018

Medicinal Research Reviews

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Many research projects are underway to improve the diagnosis and therapy in ophthalmology. Indeed, visual acuity deficits affect 285 million people worldwide and different strategies are being developed to strengthen patient care. One of these strategies is the use of gold nanoparticles (GNP) for their multiple properties and their ability to be used as both diagnosis and therapy tools. This review exhaustively details research developing GNPs for use in ophthalmology. The toxicity of GNPs and their distribution in the eye are described through in vitro and in vivo studies. All publications addressing the pharmacokinetics of GNPs administered in the eye are extensively reviewed. In addition, their use as biosensors or for imaging with optical coherence tomography is illustrated. The future of GNPs for ophthalmic therapy is also discussed. GNPs can be used to deliver genes or drugs through different administration routes. Their antiangiogenic and anti-inflammatory properties are of great interest for different ocular pathologies. Finally, GNPs can be used to improve stereotactic radiosurgery, brachytherapy, and photothermal therapy because of their many properties.

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Mapping the Glyco-Gold Nanoparticles of Different Shapes Toxicity, Biodistribution and Sequestration in Adult Zebrafish

Cited by 46 publications

References 39 publications

The biodistribution and immuno-responses of differently shaped non-modified gold particles in zebrafish embryos

The biodistribution and immuno-responses of differently shaped non-modified gold particles in zebrafish embryos

Zebrafish as a Model to Evaluate Nanoparticle Toxicity

Gold nanoparticles in ophthalmology

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