Mild hypothermia ameliorates muscle wasting in septic rats associated with hypothalamic AMPK-induced autophagy and neuropeptides

Cao, Chun; Gao, Tao; Xi, Fengchan; Zhao, Chenyan; Yu, Wenkui

doi:10.1016/j.bbrc.2017.06.135

Cited by 18 publications

(14 citation statements)

References 29 publications

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“…In our previous studies, we found hypercatabolism was caused by the elevated POMC expression during endotoxemia. 20,22 POMC could be cleaved into α-MSH and ACTH to activate the HPA axis, which is a major determinant of muscle wasting during endotoxemia. 21 And the increased cortisol is an important inducer of muscle atrophy during endotoxemia.…”

Section: Discussionmentioning

confidence: 99%

“…injecting 5‐Aminoimidazole‐4‐carboxamide1‐β‐ d ‐ribofuranoside (AICAR) or mild hypothermia to suppress the expression of POMC or knockdown POMC by i.c.v. injection of short hairpin RNA (shRNA) could significantly ameliorate hypercatabolism …”

Section: Introductionmentioning

confidence: 99%

“…injection of short hairpin RNA (shRNA) could significantly ameliorate hypercatabolism. 20,22 Therefore, the aim of this study was to compare the effect of GFs and JFs on hypercatabolism in endotoxemic rats and test whether GF activated hypothalamic AMPK-autophagy and suppressed POMC expression via gastrointestinal hormones.…”

Section: Introductionmentioning

confidence: 99%

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A Comparison of Gastric and Jejunal Feeding in Hypercatabolism Associated With Hypothalamic AMPK‐Autophagy‐POMC in Endotoxemic Rats

Cao

Li²,

Yang

et al. 2019

J Parenter Enteral Nutr

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Background Hypercatabolism is associated with increased infectious rates and mortality in critically ill patients. Enteral nutrition (EN) is usually used to counteract hypercatabolism. However, the impact of different routes of EN on hypercatabolism remains unknown. Here, we compared the impact of gastric feeding (GF) and jejunal feeding (JF) on gastrointestinal hormones and hypercatabolism, which is associated with hypothalamic adenosine 5′‐monophosphate‐activated protein kinase (AMPK)‐autophagy‐proopiomelanocortin (POMC). Methods Sixty adult male Sprague‐Dawley rats were divided into 5 groups: Sham and lipopolysaccharide (LPS) groups fed a standard chow diet, a pair‐fed group that was a subset of saline‐treated rats pair‐fed with the LPS group, and LPS + JF and LPS + GF groups (received EN via jejunal and gastric tube, respectively, for 3 days [100 kcal/kg/d]). Hypercatabolism was measured by insulin resistance, muscle protein synthesis, and atrophy. Serum gastrointestinal hormones, hypothalamic ghrelin, growth hormone secretagogue receptor‐1α (GHS‐R1α), and AMPK‐autophagy‐POMC markers were also detected. Results GF increased serum total, acylated, desacylated, and hypothalamic ghrelin and decreased glucagon‐like peptide‐1 (GLP‐1). But no effect on pancreatic polypeptide (PYY) and hypothalamic GHS‐R1α was observed. JF showed no effect on hypothalamic ghrelin, GHS‐R1α, and serum total, acylated, and desacylated ghrelin and even further aggravated GLP‐1 and PYY. GF could effectively augment hypothalamic AMPK‐autophagy‐POMC and hypercatabolism. However, JF showed no effect on hypothalamic AMPK‐autophagy‐POMC and hypercatabolism. Conclusions GF could activate hypothalamic AMPK‐autophagy and suppress POMC expression via gastrointestinal hormones to ameliorate hypercatabolism compared with JF, which suggested that GF may be the preferred route of EN in endotoxemic rats.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Comparison of Gastric and Jejunal Feeding in Hypercatabolism Associated With Hypothalamic AMPK‐Autophagy‐POMC in Endotoxemic Rats

Cao

Li²,

Yang

et al. 2019

J Parenter Enteral Nutr

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“…The ROS production was evaluated using 2,7-dichloro-uorescein diacetate (DCFDA) uorescence dye as described. 30 The cells were normally maintained, when appropriate, cells were treated with 1 mM DCFDA and incubated at 37 C for 30 minutes in dark. Aer staining, the cells were passed through a 40 mm cell strainer to get rid of cell clumps.…”

Section: Flow Cytometry Analysis Of Ros Productionmentioning

confidence: 99%

Attenuated glutamate induced ROS production by antioxidative compounds in neural cell lines

Xin

Cui

et al. 2019

RSC Adv.

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“…There are four common dimerized compounds, named theaavins (TF1), thea-avins-3-o-monogallate (TF2a), theaavins-3 0 -o-monogallate (TF2b) and theaavins-3,3 0 -o,o-digallate (TF3), all of which harbor more hydroxy groups in a single molecular. 24 Due to the ability of hydroxyl to donate electrons and hydrogen atoms, we assume both families of catechins and theaavins compounds potentially function as antioxidants. Although the biological effect of catechins were widely investigated, its dimerized compounds, theaavins were rarely studied.…”

Section: Introductionmentioning

confidence: 99%

Anti-oxidative effects of catechins and theaflavins on glutamate-induced HT22 cell damage

Yang

et al. 2019

RSC Adv.

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Mild hypothermia ameliorates muscle wasting in septic rats associated with hypothalamic AMPK-induced autophagy and neuropeptides

Cited by 18 publications

References 29 publications

A Comparison of Gastric and Jejunal Feeding in Hypercatabolism Associated With Hypothalamic AMPK‐Autophagy‐POMC in Endotoxemic Rats

A Comparison of Gastric and Jejunal Feeding in Hypercatabolism Associated With Hypothalamic AMPK‐Autophagy‐POMC in Endotoxemic Rats

Attenuated glutamate induced ROS production by antioxidative compounds in neural cell lines

Anti-oxidative effects of catechins and theaflavins on glutamate-induced HT22 cell damage

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