Expression and purification of swine RAG2 in <i>E. coli</i> for production of porcine RAG2 polyclonal antibodies

Jin, Yu-Bei; Yang, Wentao; Huang, Ke-Yan; Chen, Hongliang; Shonyela, Seria-Masole; Liu, Jing; Liu, Qiong; Feng, Bo; Zhou, You W.; Zhi, Shu-Li; Jiang, Yanlong; Wang, Jianzhong; Huang, Haibin; Shi, Chunwei; Yang, Guilian; Wang, Chunfeng

doi:10.1080/09168451.2017.1340086

Cited by 3 publications

(1 citation statement)

References 28 publications

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“…The primary immunoreagents used in this research were obtained from mouse sources. A rabbit anti-porcine RAG2 Ab that was previously prepared by our laboratory (24), which was labeled with Alexa Fluor 488 according to the manufacturer's instructions (Thermo Fisher Scientific, USA), was also used. The mouse anti-pig Abs were as follows: anti-IgM (K521C3, IgG1), anti-CD21 (BB6-11C9.6, IgG1), anti-CD2 (MSA4, IgG2a), anti-CD45RC (MIL15, IgM), anti-MHC-II (MSA3, IgG2a), and anti-CD172a (74-22-15, IgG1) (1).…”

Section: Immunoreagents and Bacteriummentioning

confidence: 99%

Lactobacillus rhamnosus GG Promotes Early B Lineage Development and IgA Production in the Lamina Propria in Piglets

Jin

Cao

Shi

et al. 2021

The Journal of Immunology

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Y.-B.J. performed CDR3 bioinformatic sequence analysis, GST pull-down assay, LC-MS/MS analysis, plasmid transfection, and Co-IP. B.F. performed the purification of p40 protein. Y.-B.J. and C.-W.S. performed the flow cytometry analysis. Y.-L.J. and H.-B.H. performed the analysis of immunofluorescence data. J.-Z.W. performed the ELISA. Y.-B.J. and W.-T.Y. designed the following experiments: Western blot, quantitative real-time PCR, flow cytometry, RNA sequencing analysis, CDR3 bioinformatic sequence analysis, GST pull-down assay, and LC-MS/MS analysis. G.-L.Y. and X.C. designed the plasmid transfection and Co-IP. Y.-B.J. wrote the final version of the paper. W.-T.Y. and X.C. modified the paper. C.-F.W. conceived and supervised the study.

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Section: Immunoreagents and Bacteriummentioning

confidence: 99%

Lactobacillus rhamnosus GG Promotes Early B Lineage Development and IgA Production in the Lamina Propria in Piglets

Jin

Cao

Shi

et al. 2021

The Journal of Immunology

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Designer Probiotics: The Next-Gen High Efficiency Biotherapeutics

Singh

Mal

Gautam

et al. 2019

Advances in Animal Biotechnology

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The probiotic engineering is a cutting edge technology for improving disease diagnosis, treating gastrointestinal disorders and infectious diseases, and improving nutrition and ecological health. Use of bioengineered microorganisms in animals has different targets and prospects owing to differences in their anatomy, physiology, and feeding habits.In ruminants, the bioengineered microorganism is primarily aimed to enhance nutrient utilization, detoxify toxic plant metabolites, and lessen the enteric methanogenesis, while in non-ruminants, the bioengineered microorganisms are aimed to enhance nutrient utilizations, confer protection against pathogens, and inhibit infectious agents. Highlights• The microorganisms can be engineered to enhance their metabolic efficiency • The bioengineered microorganisms could solve the burgeoning problem of drugresistant pathogens • The recombinant probiotics are promising therapeutic agents against infectious diseases.

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Immune responses induced by recombinant Lactobacillus plantarum expressing the spike protein derived from transmissible gastroenteritis virus in piglets

Jin

Yang

Shi

et al. 2018

Appl Microbiol Biotechnol

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Transmissible gastroenteritis coronavirus (TGEV) is one of the most severe threats to the swine industry. In this study, we constructed a suite of recombinant Lactobacillus plantarum with surface displaying the spike (S) protein coming from TGEV and fused with DC cells targeting peptides (DCpep) to develop an effective, safe, and convenient vaccine against transmissible gastroenteritis. Our research results found that the recombinant Lactobacillus plantarum (NC8-pSIP409-pgsA-S-DCpep) group expressing S fused with DCpep could not only significantly increase the percentages of MHC-IICD80 B cells and CD3CD4 T cells but also the number of IgA B cells and CD3CD4 T cells of ileum lamina propria, which elevated the specific secretory immunoglobulin A (SIgA) titers in feces and IgG titers in serum. Taken together, these results suggest that NC8-pSIP409-pgsA-S-DCpep expressing the S of TGEV fused with DCpep could effectively induce immune responses and provide a feasible original strategy and approach for the design of TGEV vaccines.

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Expression and purification of swine RAG2 in E. coli for production of porcine RAG2 polyclonal antibodies

Cited by 3 publications

References 28 publications

Lactobacillus rhamnosus GG Promotes Early B Lineage Development and IgA Production in the Lamina Propria in Piglets

Lactobacillus rhamnosus GG Promotes Early B Lineage Development and IgA Production in the Lamina Propria in Piglets

Designer Probiotics: The Next-Gen High Efficiency Biotherapeutics

Immune responses induced by recombinant Lactobacillus plantarum expressing the spike protein derived from transmissible gastroenteritis virus in piglets

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