Designer Probiotics: The Next-Gen High Efficiency Biotherapeutics

Abstract

The probiotic engineering is a cutting edge technology for improving disease diagnosis, treating gastrointestinal disorders and infectious diseases, and improving nutrition and ecological health. Use of bioengineered microorganisms in animals has different targets and prospects owing to differences in their anatomy, physiology, and feeding habits.In ruminants, the bioengineered microorganism is primarily aimed to enhance nutrient utilization, detoxify toxic plant metabolites, and lessen the enteric methanogenesis, while in non-ruminants, the bioengineered microorganisms are aimed to enhance nutrient utilizations, confer protection against pathogens, and inhibit infectious agents. Highlights• The microorganisms can be engineered to enhance their metabolic efficiency • The bioengineered microorganisms could solve the burgeoning problem of drugresistant pathogens • The recombinant probiotics are promising therapeutic agents against infectious diseases.

Recombinant Probiotic Preparations: Current State, Development and Application Prospects

“…The concept of creating recombinant probiotics appeared due to the need to find new anti-microbial agents, which, unlike antibiotics, will be safer, biodegradable, more effective against many microorganisms as well as resistant to certain groups of drugs [17,18]. Due to their primary beneficial properties (listed above), the ability to influence the microbiome and human health, probiotics are ideal objects for improving their properties or acquiring new ones via genetic engineering [11,17,77].…”

“…For example, the following medical areas of use are possible: treatment of GI disorders [79,94], as vaccines [82,92], as a means for immunomodulation [92], for disease diagnosis, as a "cell factory" to produce useful BACs, etc. [17,18,92].…”

“…As the first studies of probiotic microorganisms date back to the 19th century, the emergence of studies on the creation of recombinant probiotics appeared at the end of the 20th century. Therefore, the development of "designer" or recombinant probiotic microorganisms [18] is an indisputable breakthrough towards the creation of qualitatively new probiotic preparations with specified properties.…”