Enhancing internalization of silica particles in myocardial cells through surface modification

Ornelas‐Soto, Nancy; Rubio, Rodrigo; Guerrero-Beltrán, Carlos Enrique; Vázquez‐Garza, Eduardo; Bernal‐Ramírez, Judith; García‐García, Alejandra; Oropeza-Almazán, Yuriana; García‐Rivas, Gerardo; Contreras‐Torres, Flavio F.

doi:10.1016/j.msec.2017.05.092

Cited by 15 publications

(7 citation statements)

References 54 publications

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“…The surface area, pore volume, and N2 adsorption-desorption isotherms of HMSNAP and drug-loaded HMSNAP were analysed, and are shown in Figure 4 , whereas the mesoporous structure was explained based on a hysteresis H1-type loop [ 42 ]. The relative pressure (P/P 0 ) ranged from 0.2–1.0, confirming the monolayer absorption of HMSNAP and drug-loaded HMSNAP [ 43 ]. The respective relative pressure P/P 0 of HMSNAP, Gu-HMSNAP, C-HMSNAP, and GuC-HMSNAP were 0.8, 0.3, 0.8 and 0.2.…”

Section: Resultsmentioning

confidence: 85%

Guanidine–Curcumin Complex-Loaded Amine-Functionalised Hollow Mesoporous Silica Nanoparticles for Breast Cancer Therapy

Viswanathan

Chitradevi

Zochedh

et al. 2022

Cancers

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The current study focuses on developing a tumour-targeted functionalised nanocarrier that wraps hollow mesoporous silica nanoparticles. The guanidine carbonate and curcumin are immobilised on the surface of 3-aminopropyl-triethoxy silane (APTES)-decorated hollow mesoporous silica nanoparticles (HMSNP), as confirmed through XPS and NMR analysis. XPS analysis demonstrates that the shape of the hysteresis loops is modified and that pore volume and pore diameter are consequently decreased compared to control. Guanidine (85%) and guanidine–curcumin complex (90%) were successfully encapsulated in HMSNAP and showed a 90% effective and sustained release at pH 7.4 for up to 72 h. Acridine orange/ethidium bromide dual staining determined that GuC-HMNSAP induced more late apoptosis and necrosis at 48 and 72 h compared with Gu-HMNSAP-treated cells. Molecular investigation of guanidine-mediated apoptosis was analysed using western blotting. It was found that cleaved caspases, c-PARP, and GSK-3β (Ser9) had increased activity in MCF-7 cells. GuC-HMSNAP increased the activity of phosphorylation of oncogenic proteins such as Akt (Ser473), c-Raf (Ser249), PDK1 (Ser241), PTEN (Ser380), and GSK-3β (Ser9), thus inducing cell death in MCF-7 cells. Altogether, our findings confirm that GuC-HMNSAP induces cell death by precisely associating with tumour-suppressing proteins, which may lead to new therapeutic approaches for breast cancer therapy.

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Section: Resultsmentioning

confidence: 85%

Guanidine–Curcumin Complex-Loaded Amine-Functionalised Hollow Mesoporous Silica Nanoparticles for Breast Cancer Therapy

Viswanathan

Chitradevi

Zochedh

et al. 2022

Cancers

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“…HAP also has higher stability than liposomes and micelles, which tend to dissipate under specific concentrations [ 10 ]. Moreover, HAP is soluble and less toxic than SiO 2 , TiO 2 , quantum dots, carbon nanotubes, and other magnetic particles [ 10 , 11 , 12 , 13 ]. HAP has been the subject of study over the years in the field of biomaterials science due to its importance in clinical applications and tissue engineering [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite Nanoparticles in Drug Delivery: Physicochemistry and Applications

2021

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Hydroxyapatite (HAP) has been the gold standard in the biomedical field due to its composition and similarity to human bone. Properties such as shape, size, morphology, and ionic substitution can be tailored through the use of different synthesis techniques and compounds. Regardless of the ability to determine its physicochemical properties, a conclusion for the correlation with the biological response it is yet to be found. Hence, a special focus on the most desirable properties for an appropriate biological response needs to be addressed. This review provides an overview of the fundamental properties of hydroxyapatite nanoparticles and the characterization of physicochemical properties involved in their biological response and role as a drug delivery system. A summary of the main chemical properties and applications of hydroxyapatite, the advantages of using nanoparticles, and the influence of shape, size, functional group, morphology, and crystalline phase in the biological response is presented. A special emphasis was placed on the analysis of chemical and physical interactions of the nanoparticles and the cargo, which was explained through the use of spectroscopic and physical techniques such as FTIR, Raman, XRD, SEM, DLS, and BET. We discuss the properties tailored for hydroxyapatite nanoparticles for a specific biomolecule based on the compilation of studies performed on proteins, peptides, drugs, and genetic material.

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“…We examined cell death after 24 h of treatment using Alamar blue, and a cytotoxic effect was observed at initial concentrations of 100 μg/mL, indicating a reduced metabolic activity at those conditions. As different groups have shown, free ceramics exerts higher side effects, such as nano or microparticles, e.g., SiO2 [46,47] in different cells and tissues, even at low concentrations, as we tested with free Al2O3 (Figure 6B). Cell viability.…”

Section: Resultsmentioning

confidence: 99%

Reinforcement of Alginate-Gelatin Hydrogels with Bioceramics for Biomedical Applications: A Comparative Study

Ávila-Ramírez

Catzim-Ríos

Guerrero-Beltrán

et al. 2021

Gels

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This study states the preparation of novel ink with potential use for bone and cartilage tissue restoration. 3Dprint manufacturing allows customizing prostheses and complex morphologies of any traumatism. The quest for bioinks that increase the restoration rate based on printable polymers is a need. This study is focused on main steps, the synthesis of two bioceramic materials as WO3 and Na2Ti6O13, its integration into a biopolymeric-base matrix of Alginate and Gelatin to support the particles in a complete scaffold to trigger the potential nucleation of crystals of calcium phosphates, and its comparative study with independent systems of formulations with bioceramic particles as Al2O3, TiO2, and ZrO2. FT-IR and SEM studies result in hydroxyapatite’s potential nucleation, which can generate bone or cartilage tissue regeneration systems with low or null cytotoxicity. These composites were tested by cell culture techniques to assess their biocompatibility. Moreover, the reinforcement was compared individually by mechanical tests with higher results on synthesized materials Na2Ti6O13 with 35 kPa and WO3 with 63 kPa. Finally, the integration of these composite materials formulated by Alginate/Gelatin and bioceramic has been characterized as functional for further manufacturing with the aid of novel biofabrication techniques such as 3D printing.

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Enhancing internalization of silica particles in myocardial cells through surface modification

Cited by 15 publications

References 54 publications

Guanidine–Curcumin Complex-Loaded Amine-Functionalised Hollow Mesoporous Silica Nanoparticles for Breast Cancer Therapy

Guanidine–Curcumin Complex-Loaded Amine-Functionalised Hollow Mesoporous Silica Nanoparticles for Breast Cancer Therapy

Hydroxyapatite Nanoparticles in Drug Delivery: Physicochemistry and Applications

Reinforcement of Alginate-Gelatin Hydrogels with Bioceramics for Biomedical Applications: A Comparative Study

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