Long non-coding RNA DANCR, a prognostic indicator, promotes cell growth and tumorigenicity in gastric cancer

Abstract

Gastric cancer remains the third leading cause of cancer-related mortality worldwide, and proliferation of gastric cancer represents the major reason for its poor prognosis. Recent evidence indicates that long non-coding RNAs play crucial roles in development and progression of gastric cancer. Long non-coding RNA differentiation antagonizing non-protein coding RNA is upregulated in hepatic cell carcinoma, but the role of lncRNA differentiation antagonizing non-protein coding RNA in gastric cancer has not been explored. In this article, we found that differentiation antagonizing non-protein coding RNA is also upregulated in gastric cancer. Experiments revealed that silencing differentiation antagonizing non-protein coding RNA significantly inhibited gastric cancer cell proliferation in vitro and in vivo. Overexpression of differentiation antagonizing non-protein coding RNA notably increases gastric cancer cell proliferation. From RNA-seq and gene ontology annotations, we found that differentiation antagonizing non-protein coding RNA influences the gene expression programs in cell metabolic and cycle process. Taken together, our findings suggest that the long non-coding RNA differentiation antagonizing non-protein coding RNA promotes the proliferation of gastric cancer and is a potential prognostic biomarker and therapeutic target in gastric cancer.

Long noncoding RNA DANCR confers cytarabine resistance in acute myeloid leukemia by activating autophagy via the miR‐874‐3P/ATG16L1 axis

“…The high expression of DANCR has been linked with tumor progression and poor prognosis in colorectal cancer (Liu et al, 2015), hepatocellular carcinoma (Ma et al, 2016), and gastric cancer (Hao et al, 2017).…”

Long non‐coding small nucleolar RNA host genes in digestive cancers

“…SNHG12 could sponge MIR‐199a/b‐5p and MIR320 to promote tumorigenesis . DANCR and SNHG14 were also upregulated in gastric cancer and could promote cancer cell proliferation, invasion and migration . Mao et al described that DANCR could also regulate another lncRNA.…”

“…73,74 DANCR and SNHG14 were also upregulated in gastric cancer and could promote cancer cell proliferation, invasion and migration. [75][76][77] Mao et al described that DANCR could also regulate another lncRNA. They found DANCR inhibited the expression of NPTN-IT1 by binding with EZH2 and HDAC3.…”

<p>Meta-analysis of the prognostic value of <em>lncRNA DANCR</em> for cancer patients in China</p>

“…In accordance with the exclusion and inclusion criteria, 14 studies involving 1,117 patients were enrolled into this meta-analysis. [22][23][24][25][26][27][28][29][30][31][32][33][34][35] Characteristics of the 14 studies included in this meta-analysis are summarized in Table 1. As could be observed, the sample size in the 14 studies ranged from 34 to 135, with an average of 79.57.…”

“…26,31 All clinical pathological parameters were dependent on the pathology. Moreover, it was found that the reference genes of lncRNA DANCR were different among these studies, which had included GAPDH, [23][24][25][26][27][29][30][31][32][33][34] b-actin, 22,35 and small nuclear RNA U6. 28 Moreover, the thresholds of high and low lncRNA DANCR expression levels, including the median and average lncRNA DANCR expression, were also different among these studies.…”