Methicillin-resistant Staphylococcus aureus from infections in horses in Germany are frequent colonizers of veterinarians but rare among MRSA from infections in humans

Cuny, Christiane; Abdelbary, Mohamed M. H.; Köck, Robin; Layer, Franziska; Scheidemann, Wolfgang; Werner, Guido; Witte, Wolfgang

doi:10.1016/j.onehlt.2015.11.004

Cited by 45 publications

(49 citation statements)

References 57 publications

(102 reference statements)

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“…In addition to the MRSA CC398 t011 isolates, three MSSA CCs harbored scn : CC1 t127 ( n = 2), CC5 t701 ( n = 1), and CC5/CC130 t1943 ( n = 1). The BURP analysis grouped the spa types into five clusters and provided presumptive ST/CC information of two spa types without previously reported multi-locus sequence type: t4735 (clustering with t2420) presumably belonging to CC133 (Gómez et al, 2015), and t15816 (clustering with t549 and t3043) presumably belonging to ST1660 (Sieber et al, 2011; Cuny et al, 2016; Table 3). None of the isolates carried PVL.…”

Section: Resultsmentioning

confidence: 99%

“…mecC -positive MRSA CC130 and CC49 belonging to other spa types have been isolated from horses in France and Germany (Haenni et al, 2015; Cuny et al, 2016). MRSA CC130 is known to have a broad host spectrum, mainly ruminants among domestic animals (Paterson et al, 2012; Loncaric et al, 2013; Monecke et al, 2013; Gomez et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The apparent lack of host-specificity of t127 and thereby potential zoonotic relevance requires confirmation by high-resolution genotyping methods such as the WGS approach used in this study for CC398 isolates. The second most common spa type, t549 (ST1660), is considered a horse-adapted S. aureus clone (Cuny et al, 2016). …”

Section: Discussionmentioning

confidence: 99%

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Horses in Denmark Are a Reservoir of Diverse Clones of Methicillin-Resistant and -Susceptible Staphylococcus aureus

et al. 2017

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Denmark is a country with high prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 in pigs. Even though pig farming is regarded as the main source of human infection or colonization with MRSA CC398, 10–15% of the human cases appear not to be linked to pigs. Following the recent reports of MRSA CC398 in horses in other European countries and the lack of knowledge on S. aureus carriage in this animal species, we carried out a study to investigate whether horses constitute a reservoir of MRSA CC398 in Denmark, and to gain knowledge on the frequency and genetic diversity of S. aureus in horses, including both methicillin-resistant and -susceptible S. aureus (MSSA). Nasal swabs were collected from 401 horses originating from 74 farms, either at their farms or prior to admission to veterinary clinics. Following culture on selective media, species identification by MALDI-TOF MS and MRSA confirmation by standard PCR-based methods, S. aureus and MRSA were detected in 54 (13%) and 17 (4%) horses originating from 30 (40%) and 7 (9%) farms, respectively. Based on spa typing, MSSA differed genetically from MRSA isolates. The spa type prevalent among MSSA isolates was t127 (CC1), which was detected in 12 horses from 11 farms and represents the most common S. aureus clone isolated from human bacteremia cases in Denmark. Among the 17 MRSA carriers, 10 horses from three farms carried CC398 t011 harboring the immune evasion cluster (IEC), four horses from two farms carried IEC-negative CC398 t034, and three horses from two farms carried a mecC-positive MRSA lineage previously associated with wildlife and domestic ruminants (CC130 t528). Based on whole-genome phylogenetic analysis of the 14 MRSA CC398, t011 isolates belonged to the recently identified horse-adapted clone in Europe and were closely related to human t011 isolates from three Danish equine veterinarians, whereas t034 isolates belonged to pig-adapted clones. Our study confirms that horses carry an equine-specific clone of MRSA CC398 that can be transmitted to veterinary personnel, and reveals that these animals are exposed to MRSA and MSSA clones that are likely to originate from livestock and humans, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Horses in Denmark Are a Reservoir of Diverse Clones of Methicillin-Resistant and -Susceptible Staphylococcus aureus

et al. 2017

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“…The higher prevalence of MRSA carriage in veterinary personnel has been proven by multiple studies all over the world. The rates in Europe vary from 0.7-19.2% [8,9]. Traditionally, high prevalence data come from countries with welldeveloped livestock production, such as Netherlands, Denmark or Germany [10][11][12].…”

Section: Discussionmentioning

confidence: 99%

Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic

et al. 2020

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Background: Cases of colonization or infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) are frequently reported in people who work with animals, including veterinary personnel. The aim of this study was to determine the prevalence of MRSA colonization among veterinary professionals. A total of 134 nasal swabs from healthy attendees of a veterinary conference held in the Czech Republic were tested for presence of MRSA. The stains were further genotypically and phenotypically characterized. Results: Nine isolated MRSA strains were characterized with sequence type (ST), spa type (t) and Staphylococcal Cassette Chromosome mec type. Five different genotypes were described, including ST398-t011-IV (n = 5), ST398-t2330-IV (n = 1), ST398-t034-V (n = 1), ST225-t003-II (n = 1) and ST4894-t011-IV (n = 1). The carriage of the animal MRSA strain was confirmed in 8 cases, characteristics of one strain corresponded to the possible nosocomial origin. Among animal strains were described three spa types (t011, t034, t2330) belonging into one dominating clonal complex spa-CC11. Conclusion: According to our results, the prevalence of nasal carriage of MRSA in veterinary personnel is 6.72%. Although we described an increase compared to the results of previous study (year 2008), the prevalence in the Czech Republic is still remaining lower than reported from neighboring countries. Our results also indicate that healthcare -associated MRSA strains are still not spread among animals.

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“…Similarly, 3 pupils out of the 462 pupils sampled were observed to be colonized by CC398 and all were observed to have had history of contact with pigs or living on pig farms. The health implication of this finding is that, CC398 can serve as a potential reservoir for MRSA infection to humans (Cuny et al, 2016). …”

mentioning

confidence: 95%

Dissemination of Resistance and Virulence Determinants in Methicillin-Resistant Staphylococcus aureus During Colonization and Disease: A Review

Bitrus¹,

Zunita²,

Goni³

et al. 2018

Adv. Anim. Vet. Sci.

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Methicillin-resistant Staphylococcus aureus from infections in horses in Germany are frequent colonizers of veterinarians but rare among MRSA from infections in humans

Cited by 45 publications

References 57 publications

Horses in Denmark Are a Reservoir of Diverse Clones of Methicillin-Resistant and -Susceptible Staphylococcus aureus

Horses in Denmark Are a Reservoir of Diverse Clones of Methicillin-Resistant and -Susceptible Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic

Dissemination of Resistance and Virulence Determinants in Methicillin-Resistant Staphylococcus aureus During Colonization and Disease: A Review

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