Profiling of Campylobacter jejuni Proteome in Exponential and Stationary Phase of Growth

Turonova, Hana; Haddad, Nabila; Hernould, Michel; Chevret, Didier; Pazlarová, J.; Tresse, Odile

doi:10.3389/fmicb.2017.00913

Cited by 10 publications

(15 citation statements)

References 56 publications

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“…(2016) used proteomics to demonstrate that there were differences in the cadre of proteins expressed between microaerophilic and aerobic culture conditions. Along these lines, Turonova et al. (2017) uncovered differences in protein expression when C. jejuni was switched from the stationary to the exponential growth phase.…”

Section: Immune-based Assays For Campylobactermentioning

confidence: 95%

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

et al. 2019

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The accurate and rapid detection of Campylobacter spp. is critical for optimal surveillance throughout poultry processing in the United States. The further development of highly specific and sensitive assays to detect Campylobacter in poultry matrices has tremendous utility and potential for aiding the reduction of foodborne illness. The introduction and development of molecular methods such as polymerase chain reaction (PCR) have enhanced the diagnostic capabilities of the food industry to identify the presence of foodborne pathogens throughout poultry production. Further innovations in various methodologies, such as immune-based typing and detection as well as high throughput analyses, will provide important epidemiological data such as the identification of unique or region-specific Campylobacter. Comparable to traditional microbiology and enrichment techniques, molecular techniques/methods have the potential to have improved sensitivity and specificity, as well as speed of data acquisition. This review will focus on the development and application of rapid molecular methods for identifying and quantifying Campylobacter in U.S. poultry and the emergence of novel methods that are faster and more precise than traditional microbiological techniques.

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Section: Immune-based Assays For Campylobactermentioning

confidence: 95%

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

et al. 2019

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“…coli persister-cells were shown to experience antibiotic-induced damage, which was similar with that of non-persisters, and a post-antibiotic-exposure DNA repair activity was necessary for persisters in order to survive [36]; in an in vivo murine model, the majority of enrofloxacin-treated persisters were not the non-dividing but the moderately growing cells of Salmonella populations [37]. In C. jejuni, the molecular mechanisms behind the transition of cells to a non-growing stationary phase remain largely unknown [38]. C.…”

Section: Full-textmentioning

confidence: 99%

“…jejuni is its RpoS-independent stringent response [40]. Other regulators with a potential role in starvation stress include CosR [38] and CsrA [41], which are overexpressed in stationary phase and control important cell functions, including cell metabolism. The stationary phase cells of C.…”

Section: Full-textmentioning

confidence: 99%

Ciprofloxacin-induced persister-cells in Campylobacter jejuni

et al. 2020

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Campylobacter jejuni is a major bacterial foodborne-pathogen. Ciprofloxacin is an important antibiotic for the treatment of C. jejuni , albeit high rates of fluoroquinolone resistance have limited its usefulness. Persister-cells are transiently antibiotic-tolerant fractions of bacterial populations and their occurrence has been associated with recalcitrant and persistent bacterial infections. Here, time-kill assays with ciprofloxacin (200×MIC, 25 µg ml−1) were performed in C. jejuni strains 81–176 and RM1221 and persister-cells were found. The frequency of survivors after 8 h of ciprofloxacin exposure was approx. 10−3 for both strains, while after 22 h the frequency was between 10−5–10−7, depending on the strain and growth-phase. Interestingly, the stationary-phase cultures did not display more persister-cells compared to exponential-phase cultures, in contrast to what has been observed in other bacterial species. Persister-cells after ampicillin exposure (100×MIC, 200 µg ml−1) were not detected, implying that persister-cell formation in C. jejuni is antibiotic-specific. In attempts to identify the mechanism of ciprofloxacin persister-cell formation, stringent or SOS responses were not found to play major roles. Overall, this study reports ciprofloxacin persister-cells in C. jejuni and challenges the notion of persister-cells as plainly dormant non-growing cells.

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“…The sequence logo of CosR-binding box previously defined by Turonova et al (2017) was used to check the presence of cosRbinding box in the promoter regions of the operons encoding the efflux pumps cmeABC and macABputC among the complete genomes of C. jejuni and C. coli (Supplementary Data Sheet S1). For that, a sequence length of maximum 120 pb in the intergenic regions upstream to cmeA and macA was recovered.…”

Section: Identification Of Cosr Dna-binding Boxmentioning

confidence: 99%

Membrane Proteocomplexome of Campylobacter jejuni Using 2-D Blue Native/SDS-PAGE Combined to Bioinformatics Analysis

et al. 2020

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Campylobacter is the leading cause of the human bacterial foodborne infections in the developed countries. The perception cues from biotic or abiotic environments by the bacteria are often related to bacterial surface and membrane proteins that mediate the cellular response for the adaptation of Campylobacter jejuni to the environment. These proteins function rarely as a unique entity, they are often organized in functional complexes. In C. jejuni, these complexes are not fully identified and some of them remain unknown. To identify putative functional multi-subunit entities at the membrane subproteome level of C. jejuni, a holistic non a priori method was addressed using two-dimensional blue native/Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) in strain C. jejuni 81–176. Couples of acrylamide gradient/migration-time, membrane detergent concentration and hand-made strips were optimized to obtain reproducible extraction and separation of intact membrane protein complexes (MPCs). The MPCs were subsequently denatured using SDS-PAGE and each spot from each MPCs was identified by mass spectrometry. Altogether, 21 MPCs could be detected including multi homo-oligomeric and multi hetero-oligomeric complexes distributed in both inner and outer membranes. The function, the conservation and the regulation of the MPCs across C. jejuni strains were inspected by functional and genomic comparison analyses. In this study, relatedness between subunits of two efflux pumps, CmeABC and MacABputC was observed. In addition, a consensus sequence CosR-binding box in promoter regions of MacABputC was present in C. jejuni but not in Campylobacter coli. The MPCs identified in C. jejuni 81–176 membrane are involved in protein folding, molecule trafficking, oxidative phosphorylation, membrane structuration, peptidoglycan biosynthesis, motility and chemotaxis, stress signaling, efflux pumps and virulence.

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Profiling of Campylobacter jejuni Proteome in Exponential and Stationary Phase of Growth

Cited by 10 publications

References 56 publications

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

Ciprofloxacin-induced persister-cells in Campylobacter jejuni

Membrane Proteocomplexome of Campylobacter jejuni Using 2-D Blue Native/SDS-PAGE Combined to Bioinformatics Analysis

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