Inhibitory mechanism of five natural flavonoids against murine norovirus

Seo, Dong Joo; Choi, Changsun

doi:10.1016/j.phymed.2017.04.011

Cited by 17 publications

(9 citation statements)

References 32 publications

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“…Fisetin is a commercially available nutraceutical with anti‐inflammatory, antioxidant, anti‐tumorigenic, anti‐invasive, anti‐angiogenic, anti‐diabetic, neuroprotective and cardioprotective effects (Pal, Pearlman, & Afaq, ). Interestingly, fisetin has an anti‐noroviral activity by inducing cytokines (Seo & Choi, ). Although some of flavonoids show an antiviral effect, a molecular mechanism of their antiviral activity was rarely known.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of flavonoids as potent MERS‐CoV 3C‐like protease inhibitors

et al. 2019

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Middle East respiratory syndrome‐coronavirus (MERS‐CoV) is a zoonotic virus transmitted between animals and human beings. It causes MERS with high mortality rate. However, no vaccine or specific treatment is currently available. Since antiviral activity of some flavonoids is known, we applied a flavonoid library to probe inhibitory compounds against MERS‐CoV 3C‐like protease (3CLpro). Herbacetin, isobavachalcone, quercetin 3‐β‐d‐glucoside and helichrysetin were found to block the enzymatic activity of MERS‐CoV 3CLpro. The binding of the four flavonoids was also confirmed independently using a tryptophan‐based fluorescence method. The systematic comparison of the binding affinity of flavonoids made it possible to infer their scaffolds and functional groups required to bind with MERS‐CoV 3CLpro. An induced‐fit docking analysis revealed that S1 and S2 sites play a role in interaction with flavonoids. The experimental and computational study showed that flavonol and chalcone are favourite scaffolds to bind with the catalytic site of MERS‐CoV 3CLpro. It was also deduced that some flavonoid derivatives with hydrophobic or carbohydrate attached to their core structures have a good inhibitory effect. Therefore, we suggest that flavonoids with these characteristics can be used as templates to develop potent MERS‐CoV 3CLpro inhibitors.

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Section: Discussionmentioning

confidence: 99%

Characteristics of flavonoids as potent MERS‐CoV 3C‐like protease inhibitors

et al. 2019

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“…However, the phenolic composition and amounts vary between the various fruit types, where cranberries mainly contain A‐type proanthocyanidins. Also, Seo and Choi () showed that pretreatment with fisetin (50 μmol l −1 ), fisetin (100 μmol l −1 ), EGCG (100 μmol l −1 ), quercetin (100 μmol l −1 ), daidzein (50 μmol l −1 ) and ECG (150 μmol l −1 ), five flavonoids, significantly reduced MNV titre by 50·00 ± 7·14 to 60·67 ± 9·26%. Authors attributed the antiviral effects of flavonoids to upregulation of expression of antiviral cytokines (IFN‐α, IFN‐λ and TNF‐α) and interferon‐stimulating genes (Mx and ZAP).…”

Section: Discussionmentioning

confidence: 98%

Radiosensitivity increase in FCV-F9 virus using combined treatments with natural antimicrobials and γ-irradiation

Gobeil¹,

Shankar²,

Lacroix³

2020

Journal of Applied Microbiology

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Aims The objective was to evaluate the possible synergistic effect of cranberry juice (CJ) and commercial citrus extract (BS) against FCV‐F9 viral titre in vitro in combination with γ‐irradiation and to determinate the D10 values and radiosensitivity increase. Methods and Results Virus samples were treated with a formulation containing a mixture of BS or CJ. Results showed a D10 of 0·05, 0·42% and 1·34 kGy for the virus treated with the BS, the CJ and the irradiation alone respectively. Concentrations needed to reduce 6 log TCID50 ml−1 of viral titre were BS‐0·3%, CJ‐2·52% and 8·04 kGy. Irradiation combined with BS‐0·01% and CJ‐0·1% against FCV‐F9 virus showed D10 values of 0·74 and 0·72 kGy, respectively, resulting in a viral radiosensitization of 1·28 and 1·50 for respective treatments. Conclusion The higher viral radiosensitization observed after combining γ‐irradiation with BS‐0·01% and CJ‐0·1% indicates that CJ and BS could be used as antiviral agents alone or in combination with γ‐irradiation to prevent NoV outbreaks. Significance and Impact of the Study Cranberry juice and BS could be used in hurdle approaches in combined treatment with γ‐irradiation to assure food safety without a detrimental effect on nutritional value and maintain low processing cost.

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“…In a study by Seo and Choi (43), mouse raw 264.7 macrophage cells were treated with five flavonoids including EGCG, quercetin, fisetin, daidzein and ECG. According to this, the release of antiviral agents was regulated up by all flavonoids and neuroviruses were significantly inhibited (43). Polyphenols in olive oil provide bacterial inhibition.…”

Section: Anti-microbial Effectmentioning

confidence: 88%

The Importance of Polyphenols as Functional Food in Health

Bayır¹,

Aksoy²,

Koçyiğit³

2019

Bezmialem Science

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Polyphenols which are produced by plants are very important functional foods in our nutrition. Because of their diverse chemical structures, they are subject to main classification among them. The main groups of polyphenols are; flavanoids, lignans, stilbenes and phenolic acids. At the present time, the health effects of them have been investigated in several in vivo and in vitro studies. In addition to their antioxidant effects, they are known to have a pro-oxidant character. Polyphenols have important effects in protecting the body against external factors and the cleansing of reactive oxygen species which occur as a consequence of some diseases. Furthermore, polyphenols have effects on protection from specific diseases and stop their progression with certain mechanisms. In this review, various effects of polyphenols including antioxidant, pro-oxidant, cytotoxic, anti-inflammatory, antihypertensive, anti-diabetic are discussed which obtained from in vitro, in vivo experimental animal and clinical investigations.

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Inhibitory mechanism of five natural flavonoids against murine norovirus

Cited by 17 publications

References 32 publications

Characteristics of flavonoids as potent MERS‐CoV 3C‐like protease inhibitors

Characteristics of flavonoids as potent MERS‐CoV 3C‐like protease inhibitors

Radiosensitivity increase in FCV-F9 virus using combined treatments with natural antimicrobials and γ-irradiation

The Importance of Polyphenols as Functional Food in Health

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