Abstract:Circular RNAs (circRNAs) are a new class of noncoding RNAs. However, the expression profile and clinical significance of circRNAs in human gastric cancer is unclear. The global circRNA expression profile in human gastric cancer was measured by circRNA microarray. Hsa_circ_0014717, one of the most downregulated circRNAs in microarray, was selected as a targeted circRNA to explore its levels in gastric tissues and gastric juice. Freeze-thaw experiment and incubation experiment confirmed the stability of gastric … Show more
“…It was demonstrated that circRNAs are involved in the development of several types of diseases, such as atherosclerosis, myocardial Infarction and neuron injury [10][11][12]. Indeed, there has been a special outpouring of concern for immense amounts of research shown that circRNAs are associated with many human cancers, such as hepatocellular carcinoma [13], esophageal squamous cell carcinoma [14], human gastric cancer [15], colorectal cancer [16]. Our previous study showed that circRNA alterations are involved in resistance to avian leucosis virus subgroup-J-induced tumor formation in chickens [17], however, no reports showed that circRNAs play a key role in chicken cancer which was induced by avian leucosis virus subgroup-J (ALV-J).…”
ALV-J (subgroup J avian leucosis virus) is a kind of oncogenic exogenousretrovirus and diseases associated with ALV-J have caused severe reproduction problems in the poultry industry worldwide. However, the pathogenesis of ALV-Jinduced tumor formation is still unclear. In recent years, circRNAs are validated to be related to cancer, which prompts us to explore whether aberrant expression of circRNAs exists in ALV-J-infected tumor samples. In this study, a total of 2822 differentially expressed circRNAs were detected by RNA sequencing in the tumor livers of ALV-J-susceptible chickens (n = 3) and the normal liver samples of ALV-J-resistant chickens (n = 3), among which 2808 circRNAs in the tumor liver samples were detected as unregulated compared to those in the normal liver samples. As circRNAs are considered as miRNA sponge, we predicted top 5 miRNA targets for 24 selected upregulated circRNAs in the ALV-J-induced tumor livers. CircRNA-miRNA-mRNA regulatory axes were also constructed to provide clear interaction relationship among them. Furthermore, Gene Ontology and KEGG pathway analyses were performed for miRNA target genes, the result of which implied that metabolism plays a key role in circRNAs alterations in ALV-J-induced tumor livers. In summary, we identified that circRNAs are involved in ALV-J-induced tumor formation in chickens. However, the pathogenicity mechanism still needs to be further explored.
“…It was demonstrated that circRNAs are involved in the development of several types of diseases, such as atherosclerosis, myocardial Infarction and neuron injury [10][11][12]. Indeed, there has been a special outpouring of concern for immense amounts of research shown that circRNAs are associated with many human cancers, such as hepatocellular carcinoma [13], esophageal squamous cell carcinoma [14], human gastric cancer [15], colorectal cancer [16]. Our previous study showed that circRNA alterations are involved in resistance to avian leucosis virus subgroup-J-induced tumor formation in chickens [17], however, no reports showed that circRNAs play a key role in chicken cancer which was induced by avian leucosis virus subgroup-J (ALV-J).…”
ALV-J (subgroup J avian leucosis virus) is a kind of oncogenic exogenousretrovirus and diseases associated with ALV-J have caused severe reproduction problems in the poultry industry worldwide. However, the pathogenesis of ALV-Jinduced tumor formation is still unclear. In recent years, circRNAs are validated to be related to cancer, which prompts us to explore whether aberrant expression of circRNAs exists in ALV-J-infected tumor samples. In this study, a total of 2822 differentially expressed circRNAs were detected by RNA sequencing in the tumor livers of ALV-J-susceptible chickens (n = 3) and the normal liver samples of ALV-J-resistant chickens (n = 3), among which 2808 circRNAs in the tumor liver samples were detected as unregulated compared to those in the normal liver samples. As circRNAs are considered as miRNA sponge, we predicted top 5 miRNA targets for 24 selected upregulated circRNAs in the ALV-J-induced tumor livers. CircRNA-miRNA-mRNA regulatory axes were also constructed to provide clear interaction relationship among them. Furthermore, Gene Ontology and KEGG pathway analyses were performed for miRNA target genes, the result of which implied that metabolism plays a key role in circRNAs alterations in ALV-J-induced tumor livers. In summary, we identified that circRNAs are involved in ALV-J-induced tumor formation in chickens. However, the pathogenicity mechanism still needs to be further explored.
“…CircRNAs are widely detected in blood, saliva and body fluid, and their abundance is higher compared with linear RNAs 53 54. Besides, it was reported that circRNA was extremely stable and frequent freeze–thaw does not affect its biological property 55. Recently, studies have indicated that the superiority of circRNAs as prognosis biomarker could reflect the stage characteristics of tumourigenesis 39 48.…”
These findings together imply that could be considered as a potential HCC prognosis biomarker. Future in-depth research is required to further illustrate the involvement of in HCC tumourigenesis and metastasis.
“…Compared with paired adjacent non-tumorous tissues, hsa_circ_0003159 expression was significantly down-regulated in gastric cancer tissues and significantly negatively associated with gender, distal metastasis, and tumor-node-metastasis stage [34]. Similarly, hsa_circ_0014717 was significantly down regulated as well in gastric cancer tissues and related to tumor stage, distal metastasis, tissue carcinoembryonic antigen and carbohydrate antigen 19-9 expression [35]. However, some circRNAs are upregulated in GC tissues and play a vital role in promoting the development of gastric cancer.…”
Section: Correlation Of Circrnas To Gastric Cancermentioning
It has been confirmed that noncoding RNAs (ncRNAs) play an important role in cellular development, differentiation, proliferation and apoptosis of various carcinomas. As a member of the ncRNAs family, circular RNAs (circRNAs) are getting new research hotspots because of their newly discovered and numerous potential functions such as acting as microRNA (miRNA) sponges and binding to RNA-binding proteins (RBPs) to modulate the gene transcription. Herein, we review the current understanding of the roles of circRNAs in the clinical application of digestive system neoplasm and provide a new insight into the potential implications in the diagnosis and targeted therapy of digestive system carcinomas.
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