Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus

Abstract

It was possible to detect HBV DNA using all devices tested, but higher number of positive samples was observed when samples were collected using Salivette device, which shows high concordance to viral load observed in the paired serum samples.

Applicability of Oral Fluid and Dried Blood Spot for Hepatitis B Virus Diagnosis

“…In 2012, Portilho et al [65] evaluated two oral fluid collectors, Salivette and Chembio and three in-house qualitative PCR protocols for detecting HBV DNA and found that Salivette along to PCR for Core gene presented the most satisfactory results (sensitivity of 20 copies of HBV DNA/mL) showing that mechanical friction not always generate the best results. Furthermore, the same authors compared four collection methods (Salivette, spitting, DNA-Sal and FTA Cards) for HBV DNA detection using a qualitative in-house PCR for polymerase gene among samples from chronic carriers of HBV [24]. In this study, best results were obtained using Salivette device, in particular, among individuals presenting HBeAg and high HBV DNA viral load in serum.…”

COVID-19 salivary signature: diagnostic and research opportunities

“…Several protocols and approaches are available for DNA and RNA extraction and antibody detection, providing good performances regardless of sampling technique. 11 The diagnostic topic of saliva (called 'Salivaomics') includes the study of salivary proteins (proteomics), the study of salivary RNAs (transcriptomics), the study of salivary metabolites (metabolomics), the study of salivary microRNAs (microRNA) and the study of salivary microbiota (microbiome). 12 To date, saliva is used for the diagnosis of several diseases including hereditary diseases, autoimmune diseases, malignancies, infections, dental caries and periodontal disease.…”

USEFULNESS OF SALIVA SAMPLES FOR DETECTING SARS-CoV-2 RNA AMONG LIVER DISEASE PATIENTS

“…Saliva samples could be useful specimens since collection is less invasive, safer and allows the option of self-collection. Saliva samples have been evaluated for detecting viral hepatitis markers ( 2 , 3 ), however there is a lack of studies about usefulness of these samples for detecting SARS CoV-2 in hepatitis infected individuals and non-severe cases of COVID-19. The saliva collection can be safer than NPS samples, especially for those patients that presenting decompensated cirrhosis or other severe sequels, like hepatocarcinoma.…”

“…First, we evaluated extraction method and limit of detection of artificially spiked SARS-CoV-2 saliva samples (estimated viral load: 10 3 , 10 2 , 10 1 , 10 0 copies/mL). Saliva were collected using Salivette Device as previous described ( 3 ). These samples were tested in triplicate using two extraction methods (M1: PureLink RNA Mini Kit, Thermo Fisher Scientific, Waltham, USA and M2: QIAamp Viral RNA Mini Kit, QIAGEN, Germany) following manufacturer's recommendations with some modifications (low elution volume) along to real time PCR that amplifies N1 and N2 regions (2019-nCoV CDC EUA Kit, Integrated DNA Technologies, Coralville, USA) ( 4 ).…”