Expression and regulation of interleukin 6 and its receptor at the maternal-conceptus interface during pregnancy in pigs

Yoo, Inkyu; Han, Jisoo; Kim, Minjeong; Jang, Hyejeong; Sa, Soo-Jin; Choi, Suna; Ka, Hakhyun

doi:10.1016/j.theriogenology.2017.04.007

Cited by 12 publications

(14 citation statements)

References 33 publications

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“…Among these, IL6 and TNFα contribute to local in ammatory reactions related to conceptus implantation [64,65]. Both the endometrium and myometrium of the pig uterus express IL6R and TNFR mRNA during pregnancy [27,66,67]. In the present study, concentrations of IL6R, TNFRSF1A, and TNFRSF1B transcripts in the endometrium increased after day 10 of gestation, which is partly consistent with previously published data showing a stimulatory effect of conceptus estrogens on the expression of TNFα receptors [66] but not on IL6R [67].…”

Section: Discussionsupporting

confidence: 92%

The Alterations of Endometrial and Myometrial Receptivity in Early Pregnant Gilts in Response to Estrus Induction With PMSG/hCG

Szymańska

Blitek

2021

Preprint

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Background: The hormonal control of ovulation has become a standard procedure in the swine industry. However, exogenous gonadotropins can be detrimental to reproductive function, affecting follicle development, corpus luteum formation, and embryo development and survival. Much less is known about uterine receptivity in gilts with induced estrus. Therefore, our objective was to determine the effect of estrus induction with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) on the expression of steroid, prostaglandin, cytokine, and oxytocin receptors, as well as nuclear factor kappa B subunit 1 (NFKB1), peroxisome proliferator activated receptor gamma (PPARG), and gap junction protein alpha 1 (GJA1), in the endometrium and myometrium of early pregnant gilts. Twenty prepubertal gilts received 750 IU PMSG and 500 IU hCG 72 h later, while eighteen prepubertal gilts in the control group were observed daily for estrus behavior. All gilts were inseminated in their first estrus and slaughtered on days 10, 12, and 15 of pregnancy to collect uterine tissues for mRNA expression analyses using real-time PCR.Results: Estrus induction did not affect progesterone receptor expression in either uterine tissue. In the endometrium, greater mRNA expression of estrogen receptors (ESR1 and ESR2), androgen receptor (AR), prostaglandin (PG) E2 receptors (PTGER2 and PTGER4), PGF2α receptor (PTGFR), interleukin 6 receptor (IL6R), tumor necrosis factor α receptors (TNFRSF1A and TNFRSF1B), and oxytocin receptor (OXTR) was detected in the control than in the PMSG/hCG-treated gilts (P < 0.05). In the myometrium, concentrations of AR, PTGER2, PTGFR, and NFKB1 transcripts were lower, while PGI2 receptor and PPARG transcripts were elevated in gilts with gonadotropin-induced estrus as compared with naturally ovulated gilts (P < 0.05). Furthermore, the administration of PMSG/hCG resulted in the greater expression of GJA1 mRNA in both the endometrium and myometrium of day 15 pregnant gilts (P < 0.05). Conclusions: Estrus induction with PMSG/hCG in prepubertal gilts may affect steroid, prostaglandin, cytokine, and oxytocin receptor expression in the endometrium and myometrium, thereby altering uterine receptivity to local or systemic factors. This may, in turn, contribute to disorders in embryo-maternal interactions and the process of implantation.

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Section: Discussionsupporting

confidence: 92%

The Alterations of Endometrial and Myometrial Receptivity in Early Pregnant Gilts in Response to Estrus Induction With PMSG/hCG

Szymańska

Blitek

2021

Preprint

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“…Because LIF increases cell attachment and viability of porcine trophoblast cells in vitro [14] and was most expressed during mid- to late pregnancy, it is likely that LIF plays an important role in placental development and cell adhesion between the trophoblast and endometrial epithelial cells during mid- to late stages of pregnancy. The endometrial expression of GP130 , which forms a heterodimeric LIF receptor with LIFR, also increases during mid-pregnancy with the greatest expression on day 60 of pregnancy in pigs [18]. Based on the expression pattern of endometrial LIF during pregnancy, it is likely that the function of LIF for conceptus implantation in pigs is not as critical as in humans and rodents.…”

Section: Discussionmentioning

confidence: 99%

“…In pigs, the expression of LIF and its receptor has been reported in the endometrium. LIF is expressed in the endometrium between days 10 and 18 of the estrous cycle and early pregnancy [13,14], and LIFR and GP130 are expressed in the endometrium during the estrous cycle and pregnancy and in the placenta [15–18]. However, the expression of LIF and LIFR in the endometrium throughout all stages of the estrous cycle and pregnancy has not been determined.…”

Section: Introductionmentioning

confidence: 99%

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Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy

Yoo

Chae

Han

et al. 2019

Asian-Australas J Anim Sci

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“…IL-6 participates in embryo-uterine interactions, and is helpful for successful implantation of conceptus during early pregnancy in pigs (Blitek et al, 2012). It has been reported that IL-6 and its receptor are upregulated dramatically in the endometrial tissues during mid-to late-pregnancy and decreased at term, but the expression of IL-6 mRNA is lower in the porcine uterine endometrium in pregnant group than that during the estrous cycle on days 12 and 15 postestrus (Yoo et al, 2017). In mice, the expression of IL-6 is the highest in the proestrus phase, and is influenced by administration of estrogen and P4 (De et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Differential expression of T helper cytokines in the liver during early pregnancy in sheep

Yang¹,

Bai²,

Zhao³

et al. 2019

Anim Reprod

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Liver plays important roles in the innate and adaptive immunity, and contributes to the maternal immune adjustments during pregnancy in mice and rats. T helper 1 (Th1) and Th2 cytokines are related to immune response. However, expression of Th1 and Th2 cytokines in maternal livers is unclear during early pregnancy in sheep. In this study, livers were collected on day 16 of the estrous cycle and on days 13, 16 and 25 of pregnancy (n = 6 for each group) in ewes, and qRT-PCR, western blot and immunohistochemistry were used to analyze the expression of Th1 and Th2 cytokines in the livers. Our results showed that interferon-gamma (IFN-γ), interleukin (IL)-2, IL-4, IL-6 and IL-10 were downregulated, and IL-5 was upregulated in the livers during early pregnancy. Furthermore, there was no effect for early pregnancy on expression of TNF-β in the livers, and the IFN-γ protein was limited to the endothelial cells of the proper hepatic arteries and portal veins. In conclusion, early pregnancy exerted its effect on the liver to regulate the Th cytokines expression, but there was no evident shift from Th1 to Th2 cytokines, which may be necessary for the maternal hepatic immune adjustments during early pregnancy in sheep.

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Expression and regulation of interleukin 6 and its receptor at the maternal-conceptus interface during pregnancy in pigs

Cited by 12 publications

References 33 publications

The Alterations of Endometrial and Myometrial Receptivity in Early Pregnant Gilts in Response to Estrus Induction With PMSG/hCG

The Alterations of Endometrial and Myometrial Receptivity in Early Pregnant Gilts in Response to Estrus Induction With PMSG/hCG

Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy

Differential expression of T helper cytokines in the liver during early pregnancy in sheep

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