Evaluation of Five Different Desensitizers: A Comparative Dentin Permeability and SEM Investigation In Vitro

Yilmaz, Nasibe Aycan; Ertaş, Ertan; Oruçoğlu, Hasan

doi:10.2174/1874210601711010015

Cited by 14 publications

(10 citation statements)

References 40 publications

(52 reference statements)

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“…The results from the optical assessment were qualitatively supported by the findings from the spectrometric assessment: While methylene blue penetrated the untreated dentine discs and stained the water in the measure glasses in higher concentrations, staining was low for the test groups and especially for treatment with DentinoCer. Principally, the fact that the application of desensitizing agents allows for residual dentine permeability is in accordance with the respective literature [ 23 , 24 ]. The reason for the fact that the penetration maximum is at day 2–4 for the untreated discs and the discs of group B before concentrations decline to zero after day 8–12, is unknown.…”

Section: Discussionsupporting

confidence: 88%

Performance of a bioglass-based dentine desensitizer under lactid acid exposition: an in-vitro study

et al. 2018

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BackgroundDentine hypersensitivity is especially frequent in patients with pronounced periodontal attachment loss. Aim of the treatment is an obstruction of the dentine tubules in order to inhibit liquid or osmotic motion, which is considered as trigger for pain sensations. Novel approaches aim for obstruction by calcium phosphate compounds in order to rely on biocompatible compounds. It was the aim of the study to optically investigate the morphology and to assess the fluid permeability of treated dentine surfaces.MethodsDentine discs were pretreated in an ultrasonic bath with 17% EDTA to clean the lumina of the dentine tubules. Samples of group A remained untreated while Seal&Protect® as a conventional desensitizer was applied for group B and DentinoCer in group C. Discs were mounted into a pulp fluid simulator (PFS) with a methylene blue solution in order to create a flow pressure of 0.5 bar. Over 12 d, discs were exposed three times per day to 0.1 M nonsaturated lactic acid. At baseline and after 2, 8 and 12 d samples were removed from PFS and prepared for SEM analysis. Tubule obstruction was assessed quantitatively using Olley scores and by qualitative description of the surface. Absorption spectrometry was used to assess the concentration of leaked methylene blue outside the samples in order to estimate dentine permeability.ResultsUntreated discs showed clean lumina of all tubules at all time points and magnifications. From day 2 onwards dentine showed exposed collagene fibers due to acid exposition. Seal&Protect® initially showed homogenous dentine surface coverage that got a more granulomatous aspect in the course of treatment time. Few samples showed sporadic tubules with open lumen at day 8 and 12. Group C showed samples with a homogeneous, even surface. Narrow slits in the superficial layer are visible from day 4 on, but the dentine surface remained invisible and dentine tubules were closed till the end of the investigation period.ConclusionOver 12 d of lactid acid exposure, samples showed complete coverage of the dentine tubules in the chosen in-vitro-model when treated with Seal&Protect® or DentinoCer.

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Section: Discussionsupporting

confidence: 88%

Performance of a bioglass-based dentine desensitizer under lactid acid exposition: an in-vitro study

et al. 2018

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“…For both superficial and deep dentin discs after 1 week, Forsterite nanoparticles added to an adhesive system had the highest mean value in comparison to Forsterite nanoparticles and Forsterite nanoparticles incorporated in glass ionomer. This could be explained by the blockage of the tubules instantly with the application of the adhesive material that has the ability to plug the open dentinal tubules as application of Self Etch adhesives topically effectively reduced dentin permeability by sealing the dentin surface with an acid-resistant hybrid layer and by occluding the tubule orifices with resin tags as reported by some studies (48,49) . This finding was in accordance with several previous studies (50,13,48) and in contradiction with studies that used different methodology for surface treatment of the dentin discs by creating a smear layer with a 600 grit silicon carbide paper and applied a drop of 10% NaOCl for 60 seconds before application of treatment materials which might have affected the patency of the tubules (51) .…”

Section: Discussionmentioning

confidence: 89%

“…It analyses the ability of potential desensitizing agents to reduce fluid flow due to tubule occlusion. Standardization of the uncovered dentin area for dentin permeability assessment was accomplished by using O-rings (48) . Thereby, the same area of the same specimen was evaluated each time to ensure reproducibility of the measurements.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Application of Forsterite Nanoparticles in Different Protocols on Permeability of Superficial and Deep Dentin

et al. 2019

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Purpose: This study evaluated and compared the effect of application of Forsterite nanoparticles in different protocols on permeability of superficial and deep dentin at different time intervals. Materials and methods: thirty human molars were used to prepare 60 dentin discs, divided into three main groups (n=20) (P); (P1): Forsterite nanoparticles alone, (P2): Forsterite nanoparticles added to an adhesive system and (P3): Forsterite nanoparticles incorporated in Glass Ionomer. The main groups were subdivided into two subgroups (n=10) according to the dentin depth (D); (D1): superficial dentin and (D2): deep dentin. Permeability was measured at three time intervals (T); (T1) baseline, (T2) after one week and (T3) after four weeks. Three wt% of forsterite nanoparticles were added to adhesive system and Glass ionomer. Teeth were sectioned to obtain superficial and deep dentin discs of; 1 mm (± 0.1mm) thickness. Modified Pashly's split chamber was used to measure dentin permeability at baseline and after application of different protocols. Representative samples were assessed with ESEM and EDAX. Results: all protocols showed permeability reduction. (P2) showed the least permeability at T2 for superficial and deep dentin discs, (P1) showed the least permeability at T3 for both superficial and deep dentin discs and (P3) showed the least permeability reduction. Conclusion: it was concluded that Forsterite nanoparticles applied in different protocols can reduce dentin permeability in superficial and deep dentin. Superior dentin permeability reduction could be achieved after one week of using Forsterite nanoparticles added to the adhesive system and four weeks after using Forsterite nanoparticles alone.

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“…Much in contrast a number of articles were published, where authors used much higher pulpal pressure that did not reflect the biologic reality. In one of the recently published articles the authors applied 1.8 bar (180 kPa) of pressure 25) . Already 20 years ago authors concluded from a sophisticated investigation that use of high pressure for determination of hydraulic conductance was not recommended because the tubular content appeared to be packed against intratubular obstacles 32) .…”

Section: Discussionmentioning

confidence: 99%

“…Several in vitro investigations 14,15,[20][21][22][23] and one doubleblinded randomized controlled 6 month clinical trial 24) have proven the efficacy of TD. However, the artificial saliva employed for the storage liquid has the ability of continuous attenuation of the dentin permeability 22,25,26) . These open questions concerning the effects of water or in artificial saliva storage of TD-or water-treated dentin specimens need to be elucidated in more detail.…”

Section: Effect Of Artificial Saliva On Permeability Of Dentin Treatementioning

confidence: 99%

Effect of artificial saliva on permeability of dentin treated with phosphate containing desensitizer measured by digital flow meter

et al. 2019

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The aim of this study was to investigate the effects of artificial saliva on permeability measured using a highly sensitive digital flow meter of dentin discs treated with a phosphate containing desensitizer compound (Teethmate desensitizer; TD). Four random groups (n=10) were treated either with TD or distilled water (DW), then stored in artificial saliva (AS) or DW for 1 day, 1 week and 1 month. Flow rates under 2 kPa pressure were calculated as percentage reduction (PR%) from the baseline. The PR% of TD/AS group was significantly lower after 1 day and 1 week, but the PR%s of 1 month groups among TD/AS, TD/DW and DW/AS were not significantly different. The SEM photograph of TD/AS group displayed that the dentin surface was densely covered with mineral deposits. Ca and phosphate ions from the artificial saliva could penetrate into the tubules and precipitate as hydroxyapatite, resulting in the reduction in permeability.

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Evaluation of Five Different Desensitizers: A Comparative Dentin Permeability and SEM Investigation In Vitro

Abstract: Abstract:Background/Objective:

Cited by 14 publications

References 40 publications

Performance of a bioglass-based dentine desensitizer under lactid acid exposition: an in-vitro study

Performance of a bioglass-based dentine desensitizer under lactid acid exposition: an in-vitro study

The Effect of Application of Forsterite Nanoparticles in Different Protocols on Permeability of Superficial and Deep Dentin

Effect of artificial saliva on permeability of dentin treated with phosphate containing desensitizer measured by digital flow meter

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