High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles

Cabrera, Mariana Paola; Assis, Caio Rodrigo Dias; Neri, David F.; Pereira, Claudete Fernandes; Soria, Fernando; Carvalho, Luiz Bezerra de

doi:10.1016/j.btre.2017.03.001

Cited by 23 publications

(21 citation statements)

References 52 publications

(51 reference statements)

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“…What was particularly interesting to our research team was the possibility of modifying the structure of DE leading to give them magnetic properties. This allows for effective removal of the immobilized enzyme from the bioreactor after its use with the use of magnetic biosorption technology [ 52 , 53 ]. In our opinion, in future studies, the magnetic DE nanoparticles technology gives an interesting opportunity for developing novel chemically modified DE carriers for enzymes immobilization.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis

et al. 2018

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The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products. Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied. Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification. The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal. Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions. The research was carried out using the Box-Behnken design. Additionally, the possibility of the re-use of both alkaline as well as enzymatic reagents was investigated. Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H2/g energy poplar (0.6 mol H2/mol TRS).

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Section: Introductionmentioning

confidence: 99%

Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis

et al. 2018

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“…Two approaches can be carried out: (1) coating them with polymers containing these chemical groups and (2) adding materials encompassing these chemical arms during or after the magnetic particle synthesis (composites). In this context, Cabrera et al [22] used the 3-aminopropyltriethoxysilane (APTES) as a silane agent to available amine groups on the surface of the magnetic diatomaceous earth (mDE). The treatment with APTES was carried out after the synthesis of the mDE particles.…”

Section: Synthesis Of Iron Oxidesmentioning

confidence: 99%

“…The immobilization of different biomolecules in magnetic material has been used for several biotechnological applications in biomedicine [5], environment [43], and food industry [22] (Figure 3). At present, magnetic bio-derivatives have found potential uses in biodiesel production.…”

Section: Biotechnological Applicationsmentioning

confidence: 99%

“…The purpose of this work was to produce magnetic diatomaceous earth nanoparticles for invertase immobilization (mDE-APTES-invertase) using an easy and low-cost method that could offer high sucrolytic activity [22]. To obtain the results of high residual specific activity (92.5%), an experimental design was made with the objective of achieving the best immobilization conditions.…”

Section: High Sucrolytic Activity By Invertase Immobilized Onto Magnementioning

confidence: 99%

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Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

Cabrera¹,

Neri²,

Soria³

et al. 2019

Applied Surface Science

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In this chapter, the authors will show different proposals of magnetic bio-derivatives and its applicability in biotechnology. The historical context of immobilized enzymes, as well as highlighting the main advantages and disadvantages of each, will be mentioned. Besides, iron oxides and composite materials will be presented as support for biomolecules immobilization. Composites are effortlessly prepared including many materials capable of providing advantages to the magnetic derivatives. Enzymes covalently linked to these magnetic particles combine their catalytic properties with reaction specificity, reusability, and possible reactor construction. In addition, proteins can also be purified by these magnetic composites containing specific ligands allowing reactors and reuses too. Some characterization techniques used to study the magnetic material and derivative immobilized will be described as well. Altogether, an engaging presentation about the interesting features of magnetic bio-derivatives will highlight their uses in biotechnology field as well as in others.

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“…Invertase immobilization by covalent bonding on magnetic particles has been performed using nanocomposites of magnetic polyvinyl alcohol microspheres [12], or chitosan-coated γ-Fe 2 O 3 [13], and in magnetic diatomaceous earth nanoparticles [14], Recently,with the aim of increase the enzyme adsorption Bayramoglu et al, [15] studied the covalent immobilization of invertase by modifying magnetic nanoparticles of Fe 3 O 4 @SiO 2 with glycidyl methacrylate (GMA). Uzun et al [16] immobilized invertase on polyamidoamine (PAMAM)magnetite nanoparticles functionalized with aminopropyltrimethoxysilane (APTMS), which were then used for successive step-by-step addition of methylacrylate and ethylenediamine to form the dendritic structure of PAMAM on the superparamagnetic nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Nanopartículas magnéticas funcionalizadas y modificadas con entrecruzamiento para mejorar la inmovilización de la invertasa

2021

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Se han desarrollado procedimientos de inmovilización con invertasa utilizando diferentes soportes. Sin embargo, las desventajas como el uso de partículas pequeñas para inmovilizaciones de invertasa en reactores de lecho compacto se están resolviendo utilizando partículas magnéticas. En este estudio, los compuestos que contienen Fe3O4 se prepararon mediante la incorporación de una capa de polisiloxano necesaria para la adsorción física de la invertasa. Además, la magnetita funcionalizada se activó con glutaraldehído y polietilenimina (PEI) con el objetivo de realizar una inmovilización covalente. Se analizó el efecto de diferentes condiciones como la relación enzima: soporte, pH y temperatura en la conservación de la invertasa. Los resultados demostraron que la relación enzima: soporte óptima es mayor para la unión covalente que para la adsorción física. El pH ideal para la enzima inmovilizada es 5,0 y la actividad enzimática se mantiene hasta 70 ° C. Los valores de km son similares en ambos métodos de inmovilización. El análisis del efecto del pH y la termoestabilidad mostró que la actividad catalítica de la invertasa no se ve afectada en comparación con la enzima libre. La inmovilización covalente muestra una mayor eficacia en el proceso de inmovilización (Fε), menos inhibición y el doble de estabilidad. Las enzimas inmovilizadas por métodos físicos y covalentes se pueden reutilizar hasta por cuatro ciclos y se pueden eliminar del medio de reacción aplicando un campo magnético externo.

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High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles

Abstract: Graphical abstract

Cited by 23 publications

References 52 publications

Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis

Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis

Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

Nanopartículas magnéticas funcionalizadas y modificadas con entrecruzamiento para mejorar la inmovilización de la invertasa

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