Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats

Ahn, Mi Young; Kim, Ban Ji; Kim, Ha Jeong; Hwang, Jae Sam; Park, Kun-Koo

doi:10.1186/s40824-017-0091-9

Cited by 8 publications

(8 citation statements)

References 47 publications

(51 reference statements)

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“…These insect N-glycans were identified polymer with hexose and N-acetylhexose in this study. Monosaccharide compositions of CaG [8] and IQG [9] have been reported. N-glycan of CaG was identified as Hex 3 (Hex = arabinose or rhamnose) HexNAc 3 dHex 1 by MS/MS spectrometry.…”

Section: Resultsmentioning

confidence: 99%

“…Total RNA was isolated from melanoma tissue of melanoma mice treated with sample GAG. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA to according to previously reported procedures [8]. For detection of target gene transcripts, we designed specific forward and reverse oligonucleotide primers using Beacon Designer software (PREMIER Biosoft, Palo Alto, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…It has been reported that dung beetle ( Catharsius molossus , Ca) GAG possesses marked anti-aging activity by reducing oxidative damage and hepato-cellular biomarker levels in aged rats [8]. As an apicultural product, bumblebee ( Bombus ignitus ) queen (IQG) glycosaminoglycan has potential efficacy for treating obesity in high-fat diet rats [9] because it can significantly decrease cellular oxidative damage by decreasing lipid malondialdehyde and protein carbonyl contents.…”

Section: Introductionmentioning

confidence: 99%

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Anti-cancer effect of dung beetle glycosaminoglycans on melanoma

Ahn

Kim

et al. 2019

BMC Cancer

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BackgroundDung beetle glycosaminoglycan is known to possess anti-aging activities. However, its anti-cancer mechanisms are not fully elucidated yet. The objective of this study was to evaluate the anti-cancer effect of insect-derived polymer dung beetle glycosaminoglycan (GAG) after intraperitoneally injecting it to melanoma mice induced by B16F10 cells.MethodsTo determine molecular mechanism involved in the anti-cancer effect of dung beetle GAG, its origin N-glycan under 3KD Dalton was assayed for melanoma cell cytotoxicity. Quantitative comparisons of adhesive molecule on extracellular matrix and activities of tissue inhibitor of metalloprotease 2 (TIMP-2) were also investigated. In vivo anti-cancer effect of dung beetle GAG on solid tumor size, survival time and gene-expression profiles was also assayed using B10F10 melanoma mice model. Mice with induced melanoma were then treated with Catharsius molossus (dung beetle) GAG (CaG) at 5 mg/kg for 8 weeks to investigate its anti-cancer effects compared to bumblebee (Bombus ignitus) queen glycosaminoglycan (IQG) and Huechys sanguinea glycosaminoglycan (HEG).ResultsThese N-glycans derived from these GAG were composed of many linear heparinoid polysaccharides, polymers with hexose and N-acetylhexose. Adminstration with these GAGs increased survival time and decreased melanoma sizes in mice, in accordance with their inhibitory effects on cell growth ratio of melanoma B16F10. In addition, treatment with N-glycans derived from theses glycosaminoglycan increased activities of TIMP-2 in HMVEC cells pretreated with TNF-alpha and in melanoma cells, suggesting that they had anti-inflammatory and anticancer activities. In DNA microarray results, compared to control, CaG treated mouse group showed upregulation of 192 genes including collagen,typeI,alpha1 (Col1a1), consistent with the highly increased in vitro extracellular matrix (ECM) adhesion on collagen 1 and up-regulation of heparanase (Hpse). After treatment with CaG, a total of 152 genes were down-regulated, including nuclear RNA export factor (Nxf3) and hyaluronan proteoglycan link protein1 (Hapln1).ConclusionsGlycosaminoglycan, CaG can strengthen ECM by increasing activity of TIMP-2 and adhesion activity on collagen known to inhibit changes of ECM, leading to tumor cell invasion and progression.Electronic supplementary materialThe online version of this article (10.1186/s12885-018-5202-z) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-cancer effect of dung beetle glycosaminoglycans on melanoma

Ahn

Kim

et al. 2019

BMC Cancer

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“…Therefore, we designed this study to determine whether insect glycosaminoglycans might have a role in treating diabetes or increasing antioxidant enzyme activity under diabetes condition using, Homo diabetic (db) mice. An insect glycosaminoglycan was been prepared and purified from black and scarlet cicada ( Huechys sanguinea ) ( 6 ). Another insect glycosaminoglycan isolated from dung beetle ( Catharsius molossus, Ca) also possesses anti-aging activities.…”

Section: Introductionmentioning

confidence: 99%

Anti-Diabetic Effects of Dung Beetle Glycosaminoglycan on db Mice and Gene Expression Profiling

et al. 2018

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Anti-diabetes activity of Catharsius molossus (Ca, a type of dung beetle) glycosaminoglycan (G) was evaluated to reduce glucose, creatinine kinase, triglyceride and free fatty acid levels in db mice. Diabetic mice in six groups were administrated intraperitoneally: Db heterozygous (Normal), Db homozygous (CON), Heuchys sanguinea glycosaminoglycan (HEG, 5 mg/kg), dung beetle glycosaminoglycan (CaG, 5 mg/kg), bumblebee (Bombus ignitus) queen glycosaminoglycan (IQG, 5 mg/kg) and metformin (10 mg/kg), for 1 month. Biochemical analyses in the serum were evaluated to determine their anti-diabetic and anti-inflammatory actions in db mice after 1 month treatment with HEG, CaG or IQG treatments. Blood glucose level was decreased by treatment with CaG. CaG produced significant anti-diabetic actions by inhiting creatinine kinase and alkaline phosphatase levels. As diabetic parameters, serum glucose level, total cholesterol and triglyceride were significantly decreased in CaG5-treated group compared to the controls. Dung beetle glycosaminoglycan, compared to the control, could be a potential therapeutic agent with anti-diabetic activity in diabetic mice. CaG5-treated group, compared to the control, showed the up-regulation of 48 genes including mitochondrial yen coded tRNA lysine (mt-TK), cytochrome P450, family 8/2, subfamily b, polypeptide 1 (Cyp8b1), and down-regulation of 79 genes including S100 calcium binding protein A9 (S100a9) and immunoglobulin kappa chain complex (Igk), and 3-hydroxy-3-methylglutaryl-CoenzymeAsynthase1 (Hmgcs1). Moreover, mitochondrial thymidine kinase (mt-TK), was up-regulated, and calgranulin A (S100a9) were down-regulated by CaG5 treatment, indicating a potential therapeutic use for anti-diabetic agent.

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“…Protein carbonyl level and induction of antioxidant enzyme ratios following GAG treatment of db/db mice were also analyzed. Dung beetle (Catharsius molossus, Ca) GAG known to exhibit marked anti-ageing activity characterized by reduced oxidative damage in aged rats [16] was used as a positive control. In this work, we have also characterized purified N-glycans for deglycosylation of GbGAG followed by MS and MS/MS (MALDI TOF MS) analysis.…”

Section: Introductionmentioning

confidence: 99%

Anti-diabetic activity of field cricket glycosaminoglycan by ameliorating oxidative stress

Ahn

Kim

et al. 2020

BMC Complement Med Ther

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Background: Field cricket (Gryllus bimaculatus) is newly emerged as an edible insect in several countries. Antiinflammatory effect of glycosaminoglycan derived from this cricket on chronic disease animal model such as diabetic mouse has not been fully investigated yet. Thus, the objective of this study was to determine the antioxidative effect of such glycosaminoglycan on diabetic mouse. Methods: To discover potential therapeutic agents, field cricket glycosaminoglycan (GbG) was tested in the present study. Its anti-oxidative activities in diabetic mice were determined based on its abilities to reduce glucose, ALT, AST, ALP, LDL-cholesterol and BUN levels. Dung beetle (C. molossus) glycosaminoglycan (CaG) was used as a positive control. Db mice were intraperitoneally administered for 1 month according to their group assignments: 1) normal (DB-Hetero); 2) control (DB-Homo); 3) 5 mg/kg treatment of CaG (CaG5); 4) 5 mg/kg treatment of GbG (GbG5); and 5) 10 mg/kg treatment of metformin (Metformin 10). Results: Blood glucose level decreased after 1st week of treatment with GbG. LDL-cholesterol and alkaline phosphatase levels were also inhibited by GbG. Markers of oxidative damage, such as protein carbonyl content and levels of hepatocellular biomarkers, were reduced in db mice treated with GbG. Especially anti-oxidative activities of catalase, superoxide dismutase and glutathione peroxidase were significantly increased in GbG treated group compared to those in the control (Db Homo). GbG was composed of heparin disaccharides. Its main N-glycan was identified as Hex 9 GlcNAc 2 (m/z 1905.7) with neutral mono-sugar mainly comprising of hexose and L (+) rhamnose by mass spectroscopy. Conclusions: Sero-biochemical and hepatocellular anti-oxidant assay results in db mice suggest that cricket (G. bimaculatus) glycosaminoglycan might possess anti-oxidative effect in diabetic state.

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Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats

Cited by 8 publications

References 47 publications

Anti-cancer effect of dung beetle glycosaminoglycans on melanoma

Anti-cancer effect of dung beetle glycosaminoglycans on melanoma

Anti-Diabetic Effects of Dung Beetle Glycosaminoglycan on db Mice and Gene Expression Profiling

Anti-diabetic activity of field cricket glycosaminoglycan by ameliorating oxidative stress

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