Co-cultivation of Chlamydomonas reinhardtii with Azotobacter chroococcum improved H2 production

Xu, Lili; Cheng, Xianglong; Wu, Shuangxiu; Wang, Quanxi

doi:10.1007/s10529-017-2301-x

Cited by 27 publications

(7 citation statements)

References 16 publications

(16 reference statements)

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“…6 ). A similar effect on improvement of H 2 production in green alga has been observed in several previously reported studies [ 38 , 39 ], which used the co-cultivation of a fermentative bacterium to reduce the O 2 content and enhance the H 2 production in these cells. This improvement in H 2 productivity of our transgenic algae was comparable with reported methods under sulfur-replete conditions [ 40 , 41 ]; however, some factors might still account for the less significant yield of photobio-H 2 production by this transgenic alga when compared with sulfur-deprived cells [ 11 , 24 – 26 ] or D1 mutants [ 34 ]: (i) although a very effective knock-down effect of amiRNA-D1 on its target gene (Fig.…”

Section: Discussionsupporting

confidence: 82%

Improved photobio-H2 production regulated by artificial miRNA targeting psbA in green microalga Chlamydomonas reinhardtii

Liu

Wang

et al. 2018

Biotechnol Biofuels

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BackgroundSulfur-deprived cultivation of Chlamydomonas reinhardtii, referred as “two-stage culture” transferring the cells from regular algal medium to sulfur-deplete one, has been extensively studied to improve photobio-H2 production in this green microalga. During sulfur-deprivation treatment, the synthesis of a key component of photosystem II complex, D1 protein, was inhibited and improved photobio-H2 production could be established in C. reinhardtii. However, separation of algal cells from a regular liquid culture medium to a sulfur-deprived one is not only a discontinuous process, but also a cost- and time-consuming operation. More applicable and economic alternatives for sustained H2 production by C. reinhardtii are still highly required.ResultsIn the present study, a significant improvement in photobio-H2 production was observed in the transgenic green microalga C. reinhardtii, which employed a newly designed strategy based on a heat-inducible artificial miRNA (amiRNA) expression system targeting D1-encoded gene, psbA. A transgenic algal strain referred as “amiRNA-D1” has been successfully obtained by transforming the expression vector containing a heat-inducible promoter. After heat shock conducted in the same algal cultures, the expression of amiRNA-D1 was detected increased 15-fold accompanied with a 73% decrease of target gene psbA. More interestingly, this transgenic alga accumulated about 60% more H2 content than the wild-type strain CC-849 at the end of 7-day cultivation.ConclusionsThe photobio-H2 production in the engineered transgenic alga was significantly improved. Without imposing any nutrient-deprived stress, this novel strategy provided a convenient and efficient way for regulation of photobio-H2 production in green microalga by simply “turn on” the expression of a designed amiRNA.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1030-2) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 82%

Improved photobio-H2 production regulated by artificial miRNA targeting psbA in green microalga Chlamydomonas reinhardtii

Liu

Wang

et al. 2018

Biotechnol Biofuels

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“…The strains in the mixed bacterial system are thus interdependent, which provides the co-culture system some advantages compared with pure culture: the robustness of the culture system is enhanced [ 77 ]; the utilization efficiency of substrates in production processes is improved [ 78 ]; the production capacity of high value-added biochemical products are increased; the costs of carbon sources are saved [ 79 ]. The co-culture system plays important roles in environmental governance and microbial cell factories and has a broad range of applications, e.g., sewage treatment [ 80 ], soil remediation [ 81 ], biodegradation [ 82 , 83 ], fatty acids production [ 84 ], sugar production [ 85 ], fuel production [ 86 , 87 ], etc.…”

Section: Enabling Technologies For Light-driven Synthetic Biologymentioning

confidence: 99%

Light-Driven Synthetic Biology: Progress in Research and Industrialization of Cyanobacterial Cell Factory

Zheng

et al. 2022

Life

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Light-driven synthetic biology refers to an autotrophic microorganisms-based research platform that remodels microbial metabolism through synthetic biology and directly converts light energy into bio-based chemicals. This technology can help achieve the goal of carbon neutrality while promoting green production. Cyanobacteria are photosynthetic microorganisms that use light and CO2 for growth and production. They thus possess unique advantages as “autotrophic cell factories”. Various fuels and chemicals have been synthesized by cyanobacteria, indicating their important roles in research and industrial application. This review summarized the progresses and remaining challenges in light-driven cyanobacterial cell factory. The choice of chassis cells, strategies used in metabolic engineering, and the methods for high-value CO2 utilization will be discussed.

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“…First, the co-culture of a growth-promoting bacterium can help increase the biomass content and respiratory rates in microalgae. [67] Second, the bacterial metabolism reduced oxygen concentration of the culture both in the headspace of the reactor and as dissolved oxygen in the culture. [68] Third, the in vitro hydrogenase activity of the microalgae in the co-culture is comparatively higher due to the sustained anaerobic conditions.…”

Section: Laboratory/research and Development Stagementioning

confidence: 99%

“…[69] Fourth, bacterial co-culture enhances the intracellular starch content of microalgae providing increased substrate for respiration and increased supply of reductant. [67,69] Another way to eliminate photosynthetic oxygen is to add chemical agents that can remove oxygen. The catch is that the chemical should not be inhibitory or toxic towards microalgal growth, since they could produce huge amounts of ROS rendering it non-viable for long term applications.…”

Section: Laboratory/research and Development Stagementioning

confidence: 99%

Biohydrogen production from microalgae—Major bottlenecks and future research perspectives

Nagarajan

Dong

Chen

et al. 2021

Biotechnology Journal

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The imprudent use of fossil fuels has resulted in high greenhouse gas (GHG) emissions, leading to climate change and global warming. Reduction in GHG emissions and energy insecurity imposed by the depleting fossil fuel reserves led to the search for alternative sustainable fuels. Hydrogen is a potential alternative energy carrier and is of particular interest because hydrogen combustion releases only water. Hydrogen is also an important industrial feedstock. As an alternative energy carrier, hydrogen can be used in fuel cells for power generation. Current hydrogen production mainly relies on fossil fuels and is usually energy and CO 2 -emission intensive, thus the use of fossil fuel-derived hydrogen as a carbon-free fuel source is fallacious. Biohydrogen production can be achieved via microbial methods, and the use of microalgae for hydrogen production is outstanding due to the carbon mitigating effects and the utilization of solar energy as an energy source by microalgae. This review provides comprehensive information on the mechanisms of hydrogen production by microalgae and the enzymes involved. The major challenges in the commercialization of microalgae-based photobiological hydrogen production are critically analyzed and future research perspectives are discussed. Life cycle analysis and economic assessment of hydrogen production by microalgae are also presented.

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Co-cultivation of Chlamydomonas reinhardtii with Azotobacter chroococcum improved H2 production

Abstract: Azotobacter chroococcum improved the H production of the co-cultures by decreasing the O content and increasing the growth and starch content of the algae and the hydrogenase activity of the co-cultures relative to those of pure algal cultures.

Cited by 27 publications

References 16 publications

Improved photobio-H2 production regulated by artificial miRNA targeting psbA in green microalga Chlamydomonas reinhardtii

Improved photobio-H2 production regulated by artificial miRNA targeting psbA in green microalga Chlamydomonas reinhardtii

Light-Driven Synthetic Biology: Progress in Research and Industrialization of Cyanobacterial Cell Factory

Biohydrogen production from microalgae—Major bottlenecks and future research perspectives

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