Transcriptome Sequencing of Dianthus spiculifolius and Analysis of the Genes Involved in Responses to Combined Cold and Drought Stress

Feng

et al. 2018

IJMS

Self Cite

Plant SWEETs (Sugars Will Eventually be Exported Transporters) affect the growth of plants by regulating the transport of sugar from source to sink and its intracellular transport between different organelles. In this study, DsSWEET17 from Dianthus spiculifolius was identified and characterized. Real-time quantitative PCR analysis revealed that the expression of DsSWEET17 was affected by exogenous application of fructose and glucose as well as under salt, osmotic, and oxidation stress. Colocalization experiments showed that the DsSWEET17-GFP (green fluorescent protein) fusion protein was localized to the FM4-64-labeled tonoplasts in Arabidopsis. Compared to the wild type, the transgenic Arabidopsis seedlings overexpressing DsSWEET17 had longer roots, greater fresh weight, and a faster root growth upon exogenous application of fructose. Furthermore, transgenic Arabidopsis seedlings had significantly higher fructose accumulation than was observed for the wild-type seedlings. The analysis of root length revealed that transgenic Arabidopsis had higher tolerance to salt, osmotic, and oxidative stresses. Taken together, our results suggest that DsSWEET17 may be a tonoplast sugar transporter, and its overexpression affects sugar metabolism and confers multiple stress tolerance in Arabidopsis.

Section: Methodsmentioning

confidence: 99%

“…Dianthus spiculifolius Schur, a perennial herbaceous flowering plant in the Caryophyllaceae family, exhibits strong resistance to cold and drought stress [ 18 ]. Moreover, D. spiculifolius exhibits a number of important application characteristics, such as a strong resistance to trampling, and a high ornamental value.…”

Section: Introductionmentioning

confidence: 99%

DsSWEET17, a Tonoplast-Localized Sugar Transporter from Dianthus spiculifolius, Affects Sugar Metabolism and Confers Multiple Stress Tolerance in Arabidopsis

Feng

et al. 2018

IJMS

Self Cite

“…Dianthus spiculifolius Schur, a perennial herbaceous plant and a member of the Caryophyllaceae, shows strong resistance to cold and drought stress [ 20 ]. In this study, we identified the DsSWEET12 gene from transcriptome data of mannitol-treated D. spiculifolius .…”

Section: Introductionmentioning

confidence: 99%

A Novel Sugar Transporter from Dianthus spiculifolius, DsSWEET12, Affects Sugar Metabolism and Confers Osmotic and Oxidative Stress Tolerance in Arabidopsis

Feng

et al. 2018

IJMS

Self Cite

Plant SWEETs (sugars will eventually be exported transporters) play a role in plant growth and plant response to biotic and abiotic stresses. In the present study, DsSWEET12 from Dianthus spiculifolius was identified and characterized. Real-time quantitative PCR analysis revealed that DsSWEET12 expression was induced by sucrose starvation, mannitol, and hydrogen peroxide. Colocalization experiment showed that the DsSWEET12-GFP fusion protein was localized to the plasma membrane, which was labeled with FM4-64 dye, in Arabidopsis and suspension cells of D. spiculifolius. Compared to wild type plants, transgenic Arabidopsis seedlings overexpressing DsSWEET12 have longer roots and have a greater fresh weight, which depends on sucrose content. Furthermore, a relative root length analysis showed that transgenic Arabidopsis showed higher tolerance to osmotic and oxidative stresses. Finally, a sugar content analysis showed that the sucrose content in transgenic Arabidopsis was less than that in the wild type, while fructose and glucose contents were higher than those in the wild type. Taken together, our results suggest that DsSWEET12 plays an important role in seedling growth and plant response to osmotic and oxidative stress in Arabidopsis by influencing sugar metabolism.

“…Dianthus spiculifolius Schur, a perennial herbaceous flowering plant in the Caryophyllaceae family, exhibits strong resistance to drought stress 19 . Moreover, D. spiculifolius exhibits a number of important application characteristics, such as adaptability to a wide range of environments, strong resistance to trampling, and high ornamental value.…”

Section: Introductionmentioning

confidence: 99%

Characterization of increased cuticular wax mutant and analysis of genes involved in wax biosynthesis in Dianthus spiculifolius

Liu

et al. 2018

Hortic Res

Self Cite

Cuticular wax formation on the surface of plant leaves is associated with drought-stress tolerance. The identification of wax biosynthesis-related genes will contribute to the genetic improvement of drought resistance in plants. In this study, we characterize a novel Dianthus spiculifolius mutant with increased cuticular wax. The mutant exhibited stronger drought resistance as indicated by less leaf wilting and death, higher leaf relative water content and water retention capacity, and slower water loss and chlorophyll extraction than did the wild type during drought treatment. In the mutant leaves, 2 730 upregulated and 2 151 downregulated differentially expressed genes (DEGs) were identified by transcriptome sequencing. A wax biosynthesis pathway of the identified DEGs was significantly enriched. Finally, three key genes (DsCER1, DsMAH1, and DsWSD1) involved in wax biosynthesis were identified and verified by qPCR. These results suggest that differential expression of DEGs involved in wax biosynthesis may be associated with the increase in cuticular wax in the mutant. Taken together, our results help elucidate wax formation patterns in D. spiculifolius. Furthermore, the DEGs involved in wax biosynthesis identified here may be valuable genetic resources for improving plant stress tolerance through increased accumulation of cuticular wax.