DsSWEET17, a Tonoplast-Localized Sugar Transporter from Dianthus spiculifolius, Affects Sugar Metabolism and Confers Multiple Stress Tolerance in Arabidopsis

Zhou, Aimin; Ma, Hongping; Feng, Shuang; Gong, Shusheng; Wang, Jingang

doi:10.3390/ijms19061564

Cited by 38 publications

(26 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bioinformatics analysis of the CmSWEET17 protein revealed that it contains six TM domains ( Figure 3B), while the equivalent SWEET17 protein in other species contains seven [31,33]. Moreover, studies in other species have found that SWEET17 is localized mainly on the vacuole membrane [31,32], whereas the results of this study revealed that, in C. morifolium, CmSWEET17 is localized on the cell membrane ( Figure 4). Whether this difference in localization is related to the absence of a TM domain in the structure of CmSWEET17 remains unknown.…”

Section: Discussionmentioning

confidence: 54%

“…There are 17 SWEET members in A. thaliana [25,30]. AtSWEET17, which belongs to the fourth subfamily [31], participates in the regulation of fructose accumulation in leaves, as demonstrated by the decrease in fructose content of leaves in sweet17-1 and sweet17-2 mutants [32]. SWEET17 expression can be induced by fructose and darkness; the fructose content, in the leaves of SWEET17 overexpression lines, has been shown to decrease sharply under cold stress [33].…”

Section: Introductionmentioning

confidence: 99%

“…SWEET17 expression can be induced by fructose and darkness; the fructose content, in the leaves of SWEET17 overexpression lines, has been shown to decrease sharply under cold stress [33]. The expression of DsSWEET17 in Dianthus spiculifolius is affected by exogenous fructose, glucose, salt stress, and osmotic stress and following its transformation into A. thaliana, root growth was promoted [31]. Transgenic lines of PtSWEET17a overexpression in poplar result in a growth promoting phenotype, which is manifested as a thicker stem diameter and increased plant height [34].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sugar Transporter, CmSWEET17, Promotes Bud Outgrowth in Chrysanthemum Morifolium

Liu

Peng

Song

et al. 2019

Genes

View full text Add to dashboard Cite

We previously demonstrated that 20 mM sucrose promotes the upper axillary bud outgrowth in two-node stems of Chrysanthemum morifolium. In this study, we aimed to screen for potential genes involved in this process. Quantitative reverse transcription (qRT)-PCR analysis of sugar-related genes in the upper axillary bud of plants treated with 20 mM sucrose revealed the specific expression of the gene CmSWEET17. Expression of this gene was increased in the bud, as well as the leaves of C. morifolium, following exogenous sucrose treatment. CmSWEET17 was isolated from C. morifolium and a subcellular localization assay confirmed that the protein product was localized in the cell membrane. Overexpression of CmSWEET17 promoted upper axillary bud growth in the two-node stems treatment as compared with the wild-type. In addition, the expression of auxin transporter genes CmAUX1, CmLAX2, CmPIN1, CmPIN2, and CmPIN4 was upregulated in the upper axillary bud of CmSWEET17 overexpression lines, while indole-3-acetic acid content decreased. The results suggest that CmSWEET17 could be involved in the process of sucrose-induced axillary bud outgrowth in C. morifolium, possibly via the auxin transport pathway.

show abstract

Section: Discussionmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sugar Transporter, CmSWEET17, Promotes Bud Outgrowth in Chrysanthemum Morifolium

Liu

Peng

Song

et al. 2019

Genes

View full text Add to dashboard Cite

show abstract

“…Further, the authors characterized the cold‐suppression CsSWEET16 gene, leading to sugars compartmentalization across the vacuole and improving cold tolerance in Arabidopsis (Wang et al, 2018). Dianthus spiculifolius DsSWEET12 and 17 affect sugars metabolism and confer osmotic and oxidative stress tolerance in Arabidopsis (Zhou et al, 2018a, 2018b). Sellami et al (2019) reported the effects of higher salt stress on Arabidopsis starch metabolism, its associated enzymes, and sugar transporters.…”

Section: The Interplay Between Sugars and Phytohormones Under Abioticmentioning

confidence: 99%

Plant sugars: Homeostasis and transport under abiotic stress in plants

Saddhe

Manuka

Suprasanna

2020

Physiologia Plantarum

197

105

View full text Add to dashboard Cite

The sessile nature of plants' life is endowed with a highly evolved defense system to adapt and survive under environmental extremes. To combat such stresses, plants have developed complex and well‐coordinated molecular and metabolic networks encompassing genes, metabolites, and acclimation responses. These modulate growth, photosynthesis, osmotic maintenance, and carbohydrate homeostasis. Under a given stress condition, sugars act as key players in stress perception, signaling, and are a regulatory hub for stress‐mediated gene expression ensuring responses of osmotic adjustment, scavenging of reactive oxygen species, and maintaining the cellular energy status through carbon partitioning. Several sugar transporters are known to regulate carbohydrate partitioning and key signal transduction steps involved in the perception of biotic and abiotic stresses. Sugar transporters such as SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTER (SWEETs), SUCROSE TRANSPORTERS (SUTs), and MONOSACCHARIDE TRANSPORTERS (MSTs) are involved in sugar loading and unloading as well as long‐distance transport (source to sink) besides orchestrating oxidative and osmotic stress tolerance. It is thus necessary to understand the structure–function relationship of these sugar transporters to fine‐tune the abiotic stress‐modulated responses. Advances in genomics have unraveled many sugars signaling components playing a key role in cross‐talk in abiotic stress pathways. An integrated omics approach may aid in the identification and characterization of sugar transporters that could become targets for developing stress tolerance plants to mitigate climate change effects and improve crop yield. In this review, we have presented an up‐to‐date analysis of the sugar homeostasis under abiotic stresses as well as describe the structure and functions of sugar transporters under abiotic stresses.

show abstract

“…The over-expression of AtSWEET16 enhances freezing tolerance, likely through its positive effect on the accumulation of sugars in the vacuole [39]. Meanwhile, the heterologous expression in A. thaliana of either of the Dianthus spiculifolius genes DsSWEET12 or DsSWEET17 affects not just the plant's sugar metabolism but also its tolerance of multiple stressors [40,41]. Here, similarly, the abundance of SWEET11 and SWEET14 transcript in the leaf and leaf sheath of the stressed KO line plants proved to be considerably lower than that in the tissues of stressed WT plants ( Figures 5C,D and 6B,C).…”

Section: Discussionmentioning

confidence: 99%

The Tolerance of Salinity in Rice Requires the Presence of a Functional Copy of FLN2

Chen

Dong

et al. 2019

Biomolecules

View full text Add to dashboard Cite

A panel of ethane-methyl-sulfonate-mutagenized japonica rice lines was grown in the presence of salinity in order to identify genes required for the expression of salinity tolerance. A highly nontolerant selection proved to harbor a mutation in FLN2, a gene which encodes fructokinase-like protein2. Exposure of wild-type rice to salinity up-regulated FLN2, while a CRISPR/Cas9-generated FLN2 knockout line was hypersensitive to the stress. Both ribulose 1,5-bisphosphate carboxylase/oxygenase activity and the abundance of the transcript generated by a number of genes encoding components of sucrose synthesis were lower in the knockout line than in wild-type plants' leaves, while the sucrose contents of the leaf and root were, respectively, markedly increased and decreased. That sugar partitioning to the roots was impaired in FLN2 knockout plants was confirmed by the observation that several genes involved in carbon transport were down-regulated in both the leaf and in the leaf sheath. The levels of sucrose synthase, acid invertase, and neutral invertase activity were distinctly lower in the knockout plants' roots than in those of wild-type plants, particularly when the plants were exposed to salinity stress. The compromised salinity tolerance exhibited by the FLN2 knockout plants was likely a consequence of an inadequate supply of the assimilate required to support growth, a problem which was rectifiable by providing an exogenous supply of sucrose. The conclusion was that FLN2, on account of its influence over sugar metabolism, is important in the context of seedling growth and the rice plant's response to salinity stress.

show abstract

DsSWEET17, a Tonoplast-Localized Sugar Transporter from Dianthus spiculifolius, Affects Sugar Metabolism and Confers Multiple Stress Tolerance in Arabidopsis

Cited by 38 publications

References 24 publications

Sugar Transporter, CmSWEET17, Promotes Bud Outgrowth in Chrysanthemum Morifolium

Sugar Transporter, CmSWEET17, Promotes Bud Outgrowth in Chrysanthemum Morifolium

Plant sugars: Homeostasis and transport under abiotic stress in plants

The Tolerance of Salinity in Rice Requires the Presence of a Functional Copy of FLN2

Contact Info

Product

Resources

About