Screening and investigation Lactobacillius spp. to improve Secale cereale silage quality

Abstract

Silage is a high-moisture-content forage that is used to feed livestock. Using silage as feed is economically feasible and suitable for cattle management. Secale cereale is considered as a high-quality forage plant. After the heading stage the biomass of S. cereale increases by up to 30%; however, lignification in the cell wall causes low fermentation efficiency and coefficient digestibility, and it may also be contaminated by mycotoxin-producing fungi and can impair the quality of the silage. Therefore, the aim of this study was to screen beneficial Lactobacillius spp. for S. cereale silage to reduce fungi contamination and to increase fibrinolytic ability in the silage. Up to 100 days fermentation period and total 180 lactic acid bacteria were isolated and only a single isolate, R4-26, showed strong acidification ability and antifungal activities against mycotoxin-producing fungus. According to quantitative RT-PCR results, the mycotoxin-producing fungal contamination was significantly reduced in the S. cereale silage containing the R4-26 isolate. In enzymatic assays, only a single isolate, R48-27 demonstrated cellulase, xylanase, chitinase and esterase activities. In conclusion, the beneficial Lactobacillius spp. strains have the ability to reduce mycotoxigenic fungi contamination and increase fibrinolytic activity to improve quality of the S. cereale silage.

Effects of inoculant application on fermentation quality and rumen digestibility of high moisture sorghum-sudangrass silage

“…In our previous study, Lactobacillus plantarum R48-27 and Lactobacillus buchneri R4-26 were isolated from rye silage and confirmed by plate assay to produce the fibrinolytic enzymes and antifungal substances, respectively (Kim et al 2017). However, the effects of these new inoculants on aerobic deterioration and nutrient digestibility of SS silage did not confirm yet.…”

Effects of wild or mutated inoculants on rye silage and its rumen fermentation indices

“…Application of lactic acid bacteria (LAB) in silage has been widely used to improve the fermentation quality [5] and digestibility of silage [6,7]. Several strains of LAB have abilities of producing antifungal [8,9] and fibrinolytic enzymes [9,10], which could increase the quality of rye silage, especially harvested at the dough stage. An antifungal substance is effec-tive to inhibit the growth of undesirable bacteria [8], whereas fibrinolytic enzymes increase the digestibility of silage by hydrolyzing structural carbohydrate (SC) [11].…”

“…In addition, esterase enzymes can break down the lignin linkage in plant cells, which is a problem in late harvested forage [11]. In previous studies, application of antifungal-producing inoculant inhibited mold contamination in silage [9], whereas application of esterase-producing inoculant increased fiber digestibility of silage [10,12].…”

Characterization of Antibacterial Strains against Kiwifruit Bacterial Canker Pathogen

“…Cellulolytic reaction media contained 4 g of KH 2 PO 4 , 2 g of CaCl 2 , 2 g of NH 4 Cl, 1 g of yeast extract, 1 g of carboxymethyl cellulose, 1 g of MgSO 4 , 0.05 g of MnSO 4 , 0.05 g of FeSO 4 , 18 g of agar per liter (final pH 7–7.4). The chitinolytic reaction media contained 5 g of chitin, 5 g of yeast extract, 0.7 g of KH 2 PO 4 , 0.5 g of K 2 HPO 4 , 0.3 g of MgSO 4 , 0.1 g of FeSO 4 , 0.1 g of NaCl, 18 g of agar per liter and adjusted final pH 6.5–7.0 (Kim et al, 2017). Proteolytic reaction media contained 10 g of skim milk powder, 18 g of agar per liter (Deepthi et al, 2012).…”

“…Bacterial spore stocks (20 μl) were inoculated with 8-mm diameter paper disks and incubated at 27°C for 5 days. Cellulolytic reaction plates were stained with 0.1% aqueous congo red solution, chitinolytic reaction plates were stained with 1% aqueous congo red solution for 15 min and both were washed completely by 1 M NaCl (Kim et al, 2017). Diameter of clear yellow (cellulolytic, chitinolytic) and transparent zones (proteolytic) were measured with three replicates.…”