Expression and prognostic significance of MYL9 in esophageal squamous cell carcinoma

Wang, Jianhua; Zhang, Lan; Huang, Shu; Xu, Jing; Zhou, Yun; Yu, Xinmiao; Luo, Rong; Wang, Zhe; Jia, Wei Hua; Zheng, Min

doi:10.1371/journal.pone.0175280

Cited by 27 publications

(31 citation statements)

References 29 publications

(39 reference statements)

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“…CSRP1 encodes a member of the cysteine-rich protein (CSRP) family, which may serve as an important biomarker of malignancy. CSRP1 is inactivated in hepatocellular carcinoma [36] , whereas MYL9 , which encodes myosin light chain 9, shows prognostic significance in esophageal squamous cell carcinoma [37] . The third gene GUCA2B encodes guanylate cyclase activator 2B (also known as uroguanylin).…”

Section: Resultsmentioning

confidence: 99%

Hybrid Method Based on Information Gain and Support Vector Machine for Gene Selection in Cancer Classification

Gao

et al. 2017

Genomics, Proteomics &Amp; Bioinformatics

106

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It remains a great challenge to achieve sufficient cancer classification accuracy with the entire set of genes, due to the high dimensions, small sample size, and big noise of gene expression data. We thus proposed a hybrid gene selection method, Information Gain-Support Vector Machine (IG-SVM) in this study. IG was initially employed to filter irrelevant and redundant genes. Then, further removal of redundant genes was performed using SVM to eliminate the noise in the datasets more effectively. Finally, the informative genes selected by IG-SVM served as the input for the LIBSVM classifier. Compared to other related algorithms, IG-SVM showed the highest classification accuracy and superior performance as evaluated using five cancer gene expression datasets based on a few selected genes. As an example, IG-SVM achieved a classification accuracy of 90.32% for colon cancer, which is difficult to be accurately classified, only based on three genes including CSRP1, MYL9, and GUCA2B.

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Section: Resultsmentioning

confidence: 99%

Hybrid Method Based on Information Gain and Support Vector Machine for Gene Selection in Cancer Classification

Gao

et al. 2017

Genomics, Proteomics &Amp; Bioinformatics

106

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“…Over-expression of some galectins such as gal1 as an anti-inflammatory mediator was previously reported in gastric ulcer [46]. There were also alterations in Myl9 levels which is mainly a protein, enhancing tumor progression [47]. Increased Myl9 and Lgals7 levels might demonstrate a tendency in the ulcer areas to progress to cancerous lesions.…”

Section: Discussionmentioning

confidence: 77%

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

Koushki

Yekta

Amiri-Dashatan

et al. 2019

BMC Complement Altern Med

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Background: Gastric ulcer is one of the most prevalent diseases worldwide. In Iranian folk medicine, Achillea wilhelmsii (AW) is used as a treatment for gastric ulcer. Previous reports also mentioned Antiulcerogenic properties for this herbal plant. This study investigated the therapeutic effects of Achillea wilhelmsii C. Koch extract on indomethacin-induced gastric lesion in rats, from both proteomic and metabolomic perspectives. Methods: The rats were divided into 4 groups. Gastric ulceration was induced by a single dose of indomethacin (45 mg/kg) by oral gavage. An amount of 800 mg/kg of AW extract was administered orally. Serum and tissue samples were collected for further investigations. The metabolomic study was performed by 1 H-NMR CPMG spectrometry. Proteomic analysis was also executed by using two dimensional gel electrophoresis (2DE) followed by liquid chromatography coupled to tandem mass spectrometry (LC-ESI/MS/MS). Real time PCR was used to confirm some of the genes. Results: The macroscopic and microscopic investigations confirmed the effectiveness of the AW extract. There was a panel of metabolites which showed alteration during gastric lesion development. The levels of some of these metabolite reversed nearly to their control values after the administration of AW extract. There were also changes in the levels of some proteins including Alb, Fabp5, Hspb1, Tagln, Lgals7, Csta and Myl9 which were reversed after AW administration. Conclusions: Our findings suggested that Achillea wilhelmsii C. Koch extract could be a potential therapy to be used for indomethacin-induced gastric lesion treatment in the future. However, further investigations are needed to confirm the results.

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“…MYLK was implicated in mediation of transcellular intravasation of breast cancer cells 41 , influencing global gene regulation in cancer 42 . High level of MYL9 expression was observed in injury, aging 43 and esophageal squamous cell carcinoma 44 . TPM1 overexpression was found in NSCLC and neuroblastoma 45 and implicated in modulation of focal adhesion and cell migration behavior 46,47 .…”

Section: Discussionmentioning

confidence: 98%

TGFβ-induced cytoskeletal remodeling mediates elevation of cell stiffness and invasiveness in NSCLC

Gladilin

Ohse

Boerries

et al. 2019

Sci Rep

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Importance of growth factor (GF) signaling in cancer progression is widely acknowledged. Transforming growth factor beta (TGFβ) is known to play a key role in epithelial-to-mesenchymal transition (EMT) and metastatic cell transformation that are characterized by alterations in cell mechanical architecture and behavior towards a more robust and motile single cell phenotype. However, mechanisms mediating cancer type specific enhancement of cell mechanical phenotype in response to TGFβ remain poorly understood. Here, we combine high-throughput mechanical cell phenotyping, microarray analysis and gene-silencing to dissect cytoskeletal mediators of TGFβ-induced changes in mechanical properties of on-small-cell lung carcinoma (NSCLC) cells. Our experimental results show that elevation of rigidity and invasiveness of TGFβ-stimulated NSCLC cells correlates with upregulation of several cytoskeletal and motor proteins including vimentin, a canonical marker of EMT, and less-known unconventional myosins. Selective probing of gene-silenced cells lead to identification of unconventional myosin MYH15 as a novel mediator of elevated cell rigidity and invasiveness in TGFβ-stimulated NSCLC cells. Our experimental results provide insights into TGFβ-induced cytoskeletal remodeling of NSCLC cells and suggest that mediators of elevated cell stiffness and migratory activity such as unconventional cytoskeletal and motor proteins may represent promising pharmaceutical targets for restraining invasive spread of lung cancer.

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Expression and prognostic significance of MYL9 in esophageal squamous cell carcinoma

Cited by 27 publications

References 29 publications

Hybrid Method Based on Information Gain and Support Vector Machine for Gene Selection in Cancer Classification

Hybrid Method Based on Information Gain and Support Vector Machine for Gene Selection in Cancer Classification

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

TGFβ-induced cytoskeletal remodeling mediates elevation of cell stiffness and invasiveness in NSCLC

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