Rapid and sensitive determination of multiple endocrine‐disrupting chemicals by ultrasound‐assisted <i>in situ</i> derivatization dispersive liquid–liquid microextraction coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Wei, Na; Zheng, Zhenjia; Wang, Yuhua; Tao, Yanduo; Shao, Yuchuan; Zhu, Shuyun; You, Jing

doi:10.1002/rcm.7865

Cited by 27 publications

(5 citation statements)

References 52 publications

(113 reference statements)

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“…The majority of the few existing multianalyte methods, measuring more than one class of estrogenic compounds in biological matrices, rely on derivatization methods to increase sensitivity. ,, We, however, aimed for a simple and quick sample preparation protocol without time intensive derivatization or sample dilution which would decrease sensitivity but nevertheless acceptable matrix effects and low LOD values. For exposome-scale studies involving the measurement of samples in a high-throughput manner, a time and cost-effective sample preparation is a prerequisite.…”

Section: Resultsmentioning

confidence: 99%

A Generic Liquid Chromatography−Tandem Mass Spectrometry Exposome Method for the Determination of Xenoestrogens in Biological Matrices

et al. 2019

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We are constantly exposed to a variety of environmental contaminants and hormones, including those mimicking endogenous estrogens. These highly heterogeneous molecules are collectively referred to as xenoestrogens and hold the potential to affect and alter the delicate hormonal balance of the human body. To monitor exposure and investigate potential health implications, comprehensive analytical methods covering all major xenoestrogen classes are needed but not available to date. Herein, we describe a liquid chromatography−tandem mass spectrometry (LC−MS/MS) method for the simultaneous determination of multiple classes of endogenous as well as exogenous estrogens in human urine, serum, and breast milk to enable proper exposure and risk assessment. In total, 75 analytes were included, whereof a majority was successfully in-house validated in the three matrices. Extraction recoveries of validated analytes ranged from 71% to 110% and limits of quantification from 0.015 to 5 μg/L, 0.03 to 14 μg/L, and 0.03 to 4.6 μg/L in urine, serum, and breast milk, respectively. The applicability of the novel method was demonstrated in proof-of-principle experiments by analyzing urine from Austrian individuals and breast milk from Austrian and Nigerian individuals. Thereby, we proved the methods' feasibility to identify and quantify different classes of xenoestrogens simultaneously. The results illustrate the general importance of multiclass exposure assessment in the context of the exposome paradigm. Specifically, they highlight the need for estimating total estrogenic burden rather than single analyte or chemical class measurements and its potential impact in endocrine disruption and hormone related diseases including cancers.

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Section: Resultsmentioning

confidence: 99%

A Generic Liquid Chromatography−Tandem Mass Spectrometry Exposome Method for the Determination of Xenoestrogens in Biological Matrices

et al. 2019

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“…At present, QuEChERS, 18,19 solid phase extraction (SPE), 20,21 disperse liquid-liquid microextraction (DLLME), 22,23 and liquid phase microextraction (LPME) 24,25 are commonly used for purication and enrichment of disinfectants in different matrices. Although these pretreatment methods have high recovery, the required sample volumes are generally large, and the operation is time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 11 antiseptic ingredients in surface water based on polypyrrole decorated magnetic nanoparticles

Zhang

Lian

et al. 2020

RSC Adv.

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“…Recently, ultrasound‐assistance is an effective way to speed up the derivative reaction. The ultrasound‐assisted reaction is characterized as economical, safe and time‐saving 13,14 . In these reactions, cavitation occurs by the passage of ultrasonic waves through a solvent, with the cavitation effect capable of promoting an interaction between a substrate and the derivatization reagent and ultimately enhancing chemical‐reaction rates.…”

Section: Introductionmentioning

confidence: 99%

Gas chromatography/mass spectrometry analysis of organic acid profiles in human serum: A protocol of direct ultrasound‐assisted derivatization

Zhang

Chou

Sun

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale Low‐molecular‐weight organic acids that generally contain one to three carboxyl groups are involved in many important biological processes; therefore, it is important to develop a quantitative method for analyzing organic acids in serum in order to allow an evaluation of metabolic changes. In this study, we evaluated a protocol for detecting 26 organic acids in serum based on ultrasound‐assisted derivatization by gas chromatography/mass spectrometry (GC/MS). Methods Serum samples were prepared using ultrasound‐assisted silane derivatization before GC/MS analysis to quantify concentrations of organic acids. Additionally, we investigated the variables affecting derivatization yields, including the extraction solvent, derivatization reagents, and derivatization conditions (reaction temperature, duration, and sonication parameters). The protocol was ultimately applied to detect organic acid profiles related to obesity. Results We used acetone as the extraction solvent and determined suitable derivatization conditions, as follows: BSTFA + 1% TMCS, 50°C, 10 min, and 100% ultrasound power. The protocol showed satisfactory linearity (r = 0.9958–0.9996), a low limit of detection (0.04–0.42 μmol/L), good reproducibility (coefficient of variation (CV) %: 0.32–13.76%), acceptable accuracy (recovery: 82.97–114.96%), and good stability within 5 days (CV%: 1.35–12.01% at room temperature, 1.24–14.09% at 4°C, and 1.01–11.67% at −20°C). Moreover, the protocol was successfully applied to obtain the organic acid profiles from obese and healthy control subjects. Conclusions We identified and validated a protocol for ultrasound‐assisted derivatization prior to GC/MS analysis for detecting 26 kinds of organic acids in serum. The results suggest the efficacy of this protocol for clinical applications to determine metabolic changes related to fluctuations in organic acid profiles.

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Rapid and sensitive determination of multiple endocrine‐disrupting chemicals by ultrasound‐assisted in situ derivatization dispersive liquid–liquid microextraction coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Cited by 27 publications

References 52 publications

A Generic Liquid Chromatography−Tandem Mass Spectrometry Exposome Method for the Determination of Xenoestrogens in Biological Matrices

A Generic Liquid Chromatography−Tandem Mass Spectrometry Exposome Method for the Determination of Xenoestrogens in Biological Matrices

Simultaneous determination of 11 antiseptic ingredients in surface water based on polypyrrole decorated magnetic nanoparticles

Gas chromatography/mass spectrometry analysis of organic acid profiles in human serum: A protocol of direct ultrasound‐assisted derivatization

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