Concept of experimental preparation for treating dentin hypersensitivity

Tanasiewicz, Marta; Gibas, Mirosław; Skucha-Nowak, Małgorzata; Twardawa, Henryk; Machorowska-Pieniążęk, Agnieszka

doi:10.1515/med-2016-0070

Cited by 3 publications

(3 citation statements)

References 10 publications

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“…Anhydrous acetone was used as the organic solvent in this preparation, constituting ≈31% of its complete volume. The comparative material was a marketable preparation Seal & Protect (Dentsply) [3].…”

Section: Methodsmentioning

confidence: 99%

“…Complex physical-chemical-biological characteristics are also an important condition for the application of such formulations. In this group of therapeutically relevant formulations, it may be important that the components have the ability to form nanoemulsions encapsulating polydimethylsiloxane oils or other neutralizing therapeutic ingredients [2,3].…”

Section: Introductionmentioning

confidence: 99%

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The Cytotoxicity Assessment of Novel Formulation Developed to Reduce Dentin Hypersensitivity Utilizing Dehydrogenase Assay

et al. 2021

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The problem of real treatment of teeth hypersensitivity is still important and unsolved. The main goal of the experiment was to calculate the possible toxic effects on the fibroblasts cells CCL-1™ (NCTC clone 929) caused by original preparation to reduce tooth surfaces’ hypersensitivity, compared to the marketable preparation Seal & Protect (Dentsply). The assessment was made through measuring lactate dehydrogenase (LDH assay). Lactate dehydrogenase releases from the cell’s cytoplasm to the culture medium as a result of cell membrane damage and lysis of the cells. The measurement is based on an assessment of the ability of LDH to oxidize lactic acid to pyruvic acid, which is dependent on the increase of the release level. The increase of LDH activity in the supernatants of cell cultures shows a relationship with the percentage of dead cells (increased cytotoxicity correlates with the increasing content of dead cells). In the LDH assay, both formulations evaluated after 24 h obtained results which were located below the control values. After seven days, the mean values obtained in cytotoxicity assay LDH are measurable and lower for the original formulation in comparison to the commercial one at the dilution of 1:5. At the dilution with 1:10 ratio, they are comparable and within the range of accepted values. At the maximal dilution of 1:15, the results are higher for the experimental formulation in comparison to the marketable formulation. The polymerization process is beneficial for the cytotoxicity test results in case of both tested preparations. Average values of cytotoxicity of both preparations attain an acceptable level of less than 22.6% ± 8.1, reliant on the degree of dilution and the remark time. Original formulation is characterized by a greater homogeneity of results. The marketable preparation has a larger diversity of effects, dependent on the time of observation and attenuation; however, the cytotoxicity values are lower when paralleled to the experimental formulation in the test conducted after seven days. This should not have a disastrous effect on the pulp, as the values of both as the values of both preparations are within expected ranges. The obtained results allows to assume that will be possible to introduce the original formulation to the stage of clinical trials in the future.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Cytotoxicity Assessment of Novel Formulation Developed to Reduce Dentin Hypersensitivity Utilizing Dehydrogenase Assay

et al. 2021

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“…The process of demineralisation of the cementum and root dentin is similar to caries within the tooth crown, but the process is twice as fast as in the enamel [6]. Another problem that occurs when the root surface is exposed, and a thin layer of cementum is lost is the hypersensitivity to thermal, chemical, and mechanical stimuli [7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Infiltrant Application in the Course of Root Cementum Caries with Different Methods of Surface Preparation—An In Vitro Study

et al. 2022

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The aim of this study is to evaluate the depth of penetration of an experimental preparation with the characteristics of a dental infiltrant into the decalcified root cementum tissue and observation of the root cementum tissue subjected to a single and repeated twice hydrochloric acid etching process. The study material consisted of 20 human teeth (the study group—12 demineralised teeth, the control group—8 teeth). A commercially available Icon preparation and an experimental preparation were used for the study with addition 2% of YF3 (yttrium trifluoride) added as an indicator to facilitate microscopic observation. Each tooth was divided into two zones, blue (Icon) and red (experimental preparation). The teeth were divided into two subgroups—in the first subgroup, the etching preparation was applied once, in the second subgroup twice, and at the end the teeth were infiltrated with the experimental preparation and the Icon preparation. The study of tooth longitudinal section morphology and chemical composition was performed with the use of a Hitachi S-3400N scanning electron microscope. Microscopic observations show that the applied YF3 tracer in most cases agglomerates and remains in the form of conglomerates on the surface of the root cementum. Single particles of YF3 are visible, penetrating through the cementum tissues into the root dentine structure. The degree of tissue infiltration with the resin (depth of penetration into decalcified tissues) is visible at a depth of approx. 80–120 μm. In the test group subjected to a single etching process, good penetration of both resins was noticeable, however, excessive erosion of the root surface was evident in several of the specimens—indicating that damage occurred as a result of the etching process. In the test group subjected to two etching processes, excessive erosion of the cementum is visible in each deposit.

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Yttrium Trifluoride as a Marker of Infiltration Rate of Decalcified Root Cementum: An In Vitro Study

et al. 2022

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Research related to the development of a dental infiltrant for minimally invasive treatment of initial caries of hard dental tissues is presented. The formulation of the developed infiltrant material includes typical methacrylate monomers used in dentistry, an author’s adhesion monomer containing metronidazole, a photoinitiating system and yttrium trifluoride (YF3). The main objective of the study was to evaluate penetration into decalcified root cementum using scanning electron microscope of an experimental preparation with the characteristics of a dental infiltrant compared to a commercial preparation with the addition of YF3 as a contrast agent. Microscopic observations showed that YF3 particles virtually did not penetrate deep into the root cementum—this was mainly due to the particle size of YF3. Observations of cementum and root dentin tissue infiltration: resin tissue infiltration was visible to a depth of about 80 to 120 μm without the use of a tracer, which, due to agglomeration and particle size, remained on the cementum surface or in the resin used for inlaying. There were no differences between the degree of penetration of an experimental preparation with the characteristics of a dental infiltrant, as compared to a commercial preparation.

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Concept of experimental preparation for treating dentin hypersensitivity

Cited by 3 publications

References 10 publications

The Cytotoxicity Assessment of Novel Formulation Developed to Reduce Dentin Hypersensitivity Utilizing Dehydrogenase Assay

The Cytotoxicity Assessment of Novel Formulation Developed to Reduce Dentin Hypersensitivity Utilizing Dehydrogenase Assay

Evaluation of Infiltrant Application in the Course of Root Cementum Caries with Different Methods of Surface Preparation—An In Vitro Study

Yttrium Trifluoride as a Marker of Infiltration Rate of Decalcified Root Cementum: An In Vitro Study

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