Functional characterization of the Arabidopsis universal stress protein AtUSP with an antifungal activity

Park, Seong-Cheol; Jung, Young Jun; Lee, Yong Jae; Kim, Il Ryong; Seol, Min-A; Kim, Young Ho; Jang, Mi-Kyeong; Lee, Jung Ro

doi:10.1016/j.bbrc.2017.03.126

Cited by 20 publications

(26 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AtUSP (AT3G53990) has been implicated in plant responses to drought (Isokpehi et al, 2011), oxidative stress (Jung et al, 2015), and heat and cold stress (Jung et al, 2015;Melencion et al, 2017). A role in plant defense against pathogens was recently documented, with AtUSP accumulating in plants exposed to Pseudomonas syringae and displaying antimicrobial activity against various fungi (Park et al, 2017). We confirmed the presence of AtUSP in both the cytoplasm and the nucleus (Melencion et al, 2017), consistent with a possible dual function for this protein.…”

Section: Discussionsupporting

confidence: 80%

The Meloidogyne incognita Nuclear Effector MiEFF1 Interacts With Arabidopsis Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases to Promote Parasitism

et al. 2021

View full text Add to dashboard Cite

Root-knot nematodes are obligate endoparasites that maintain a biotrophic relationship with their hosts over a period of several weeks. They induce the differentiation of root cells into specialized multinucleate hypertrophied feeding cells known as giant cells. Nematode effectors synthesized in the esophageal glands and injected into the plant tissue through the syringe-like stylet play a key role in giant cell ontogenesis. The Meloidogyne incognita MiEFF1 is one of the rare effectors of phytopathogenic nematodes to have been located in vivo in feeding cells. This effector specifically targets the giant cell nuclei. We investigated the Arabidopsis functions modulated by this effector, by using a yeast two-hybrid approach to identify its host targets. We characterized a universal stress protein (USP) and cytosolic glyceraldehyde-3-phosphate dehydrogenases (GAPCs) as the targets of MiEFF1. We validated the interaction of MiEFF1 with these host targets in the plant cell nucleus, by bimolecular fluorescence complementation (BiFC). A functional analysis with Arabidopsis GUS reporter lines and knockout mutant lines showed that GAPCs were induced in giant cells and that their non-metabolic functions were required for root-knot nematode infection. These susceptibility factors are potentially interesting targets for the development of new root-knot nematode control strategies.

show abstract

Section: Discussionsupporting

confidence: 80%

The Meloidogyne incognita Nuclear Effector MiEFF1 Interacts With Arabidopsis Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases to Promote Parasitism

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Another selected gene, the universal stress protein gene, encodes a protein that responds to diverse abiotic stresses, such as fever, hydrogen peroxide, salt, drought, and low temperature 22–26 . The universal stress protein isolated from Arabidopsis has an antifungal activity 27 . Here, the expression of the universal stress protein A-like protein was slightly increased (Fig.…”

Section: Discussionmentioning

confidence: 99%

Effects of trans-2-hexenal and cis-3-hexenal on post-harvest strawberry

Wakai

Kusama

Nakajima

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Green leaf volatiles are emitted by green plants and induce defence responses. Those with antifungal activities in plants may replace chemicals as natural post-harvest treatments. We investigated the postharvest treatment of strawberry with trans -2-hexenal and cis -3-hexenal and observed a decrease in the mould infection rate. To determine the volatiles’ functions, we conducted a component analysis of the volatiles released from trans -2-hexenal-treated strawberry and analysed gene expression. Several acetates, which were expected to be metabolites of trans -2-hexenal in fruit, were released from treated strawberry; however, these acetates did not inhibit fungal growth. The gene expression analysis suggested that postharvest strawberries were not protected by jasmonic acid-mediated signalling but by another stress-related protein. Harvested strawberries experience stress induced by harvest-related injuries and are unable to perform photosynthesis, which might result in different responses than in normal plants.

show abstract

“…Small blebs were observed on the surfaces of cells treated with (HKK) 1 or (HKK) 2 peptides (Figure 6d,e), and the surfaces of other HKK peptide-treated cells showed more roughening and blebbing (Figure 6f–h) than in cells treated with melittin. The antifungal mechanism of melittin has been reported to occur via formation of toroidal pores and membranolytic action in the fungal membrane [35,36,37]. The results indicated that the mode of action of HKK peptides differed from that of melittin.…”

Section: Resultsmentioning

confidence: 99%

Hydrophilic Linear Peptide with Histidine and Lysine Residues as a Key Factor Affecting Antifungal Activity

Park

Kim

et al. 2018

IJMS

Self Cite

View full text Add to dashboard Cite

Increases in the numbers of immunocompromised patients and the emergence of drug-resistance fungal pathogens have led to the need for new, safe, efficacious antifungal agents. In this study, we designed a histidine-lysine-lysine (HKK) motif and synthesized six HKK peptides with repetitions of the motif. These peptides showed length-dependent antifungal activity against drug-susceptible and drug-resistant fungal pathogens via membranolytic or non-membranolytic action. None of the peptides were cytotoxic to rat erythrocytes or NIH3T3 mouse embryonic fibroblasts. Short-length peptides were directly translocated in fungal cytosol and reacted with mitochondria, resulting in apoptosis. Membrane-permeabilizing activity occurred in the presence of long peptides, and peptides were able to transfer to the cytosol and induce reactive oxygen species. Our results suggest that peptides composed only of cationic amino acids may be good candidates as antifungal agents.

show abstract

Functional characterization of the Arabidopsis universal stress protein AtUSP with an antifungal activity

Cited by 20 publications

References 22 publications

The Meloidogyne incognita Nuclear Effector MiEFF1 Interacts With Arabidopsis Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases to Promote Parasitism

The Meloidogyne incognita Nuclear Effector MiEFF1 Interacts With Arabidopsis Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases to Promote Parasitism

Effects of trans-2-hexenal and cis-3-hexenal on post-harvest strawberry

Hydrophilic Linear Peptide with Histidine and Lysine Residues as a Key Factor Affecting Antifungal Activity

Contact Info

Product

Resources

About