Effect of Aster tataricus on production of inflammatory mediators in LPS stimulated rat astrocytoma cell line (C6) and THP-1 cells

Abstract

Neuroinflammation is the commonest cause of neurodegenerative diseases such as Alzheimer's disease. Present investigation evaluates the inhibitory effect of ethanolic root extract of (AS) on inflammatory mediators production in lipopolysaccharide (LPS) stimulated C6 cells. C6 cells were treated with AS (20 and 40 mg/kg) and nimesulide (NSL, 1.5 μg/ml) for 1 day. Thereafter various parameters such as production of ROS, release of nitrite, MDA, glutathione level and NF-κB translocation were estimated in C6 cell lines. Effect of AS was estimated on the expressions of tumor necrosis factor α (TNF-α) of human monocytic leukemia cell line (THP-1). It was observed that AS (20 and 40 mg/ml) treated group shows significant ( < 0.01) decrease in production of ROS, Nitrite release and MDA level in LPS activated C6 cell lines compared to negative control group. Moreover, treatment with it decreases glutathione level and inhibits the translocation of NF-κB in LPS activated C6 cell lines compared to negative control group. There were significant ( < 0.01) decreases in expression of TNF-α in AS treated group compared to negative control group in THP-1 cell lines. Present investigation concludes the anti neuroinflammatory effect of ethanolic extract of AS root by decreasing oxidative stress and attenuates the cytokine.

Induction of apoptosis and G1 phase cell cycle arrest by Aster�incises in AGS gastric adenocarcinoma cells

“…Other plants belonging to the Asteraceae family have been used for traditional medicinal purposes and most recently, plants from the Aster genus were studied and reported for their biological activities. Aster tataricus can reduce the damage of neurons in epileptic rats (31), inhibit oxidative stress (32), regulate the expression of inflammatory cytokines in THP-1 cells (33) and inhibit the proliferation of SCC-9 human oral squamous carcinoma (34). Aster yomena can inhibit adipogenesis in 3T3-L1 preadipocytes (35) and inflammation in RAW 264.7 macrophages (36).…”

Effects of different components of Mao Dongqing’s total flavonoids and total saponins on transient ischemic attack (TIA) model of rats

“…The traditional Chinese medicine has a long history in the treatment of cerebrovascular diseases, and has the advantages of small side effect, low cost, and so on (Jin et al, 2015). At present, Chinese medicine treatment of the disease is still dominated by the Decoction (Bai et al, 2015, Zhang et al, 2017), limit its application. Although there are pills, Huoxue Capsule and other traditional Chinese medicine, but are not exclusively used for the treatment of TIA, and the efficacy of the treatment also needs to be further improved.…”

Aster tataricus Linn., Suppresses Hepatic Stellate Cell Activation and Protects Against Thioacetamide-induced Liver Fibrosis in Rats

“…Traditionally, A. tataricus is used for medicinal and several reports have indicated the presence of numerous bioactive constituents including dietary flavonoids, triterpenes, saponins and specific constituents such as epifriedelinol, caffeoylquinic acids, shionone and aster peptides shionone, epifriedelinol, quercetin, kaempferol, scopoletin, emodin, aurantiamide acetate and 1,7-dihydroxy-6-methyl-anthraquinone [49][50][51][52] . Some of these compounds, such as quercetin, saponins, kaempferol, epifriedelinol, caffeoylquinic acids possess strong antioxidant, antiinflammatory and hepatoprotective properties including antifibrotic effects [53][54][55][56] . The compounds present in the ATE might act in a synergistic way in promoting potent antihepatofibrosis effect.…”

“…More recently, potential inhibitors were isolated from Aster tataricuswhich exhibited antiinflammatory effects bypreventing the downstream activation of the mitogen-activated protein kinase signaling pathway [20,21] . A. tataricus was found to protect retinal damage in diabetic rats due to the antioxidant and antiinflammatory effects [22] and attenuated neuroinflammation by decreasing the oxidative stress and inhibiting the cytokine production in LPS-stimulated rat astrocytoma cell line (C6) and THP-1 cells [23] . Further, A. tataricus extract was also reported to possess antiinflammatory and anticancer activity in LPS-induced acute lung injury model and by effectively attenuating the growth of SCC-9 cancer cells by virtue of its cytotoxic and anticlonogenic activity [24,25] .…”