The over expression of long non-coding RNA ANRIL promotes epithelial-mesenchymal transition by activating the ATM-E2F1 signaling pathway in pancreatic cancer: An in vivo and in vitro study

Chen, Shi; Zhang, Jiaqiang; Chen, Jiang-Zhi; Chen, Huixing; Qiu, Funan; Yan, Mao-Lin; Chen, Yanling; Peng, Chenghong; Tian, Yuan; Wang, Yaodong

doi:10.1016/j.ijbiomac.2017.03.123

Cited by 47 publications

(26 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lncRNA ANRIL has been studied in many diseases, including several types of cancer. Chen et al demonstrated that ANRIL overexpression accelerated epithelial-mesenchymal transition of pancreatic cancer cells by ATM-E2F1 signalling [19]. Wei et al showed that ANRIL improved osteosarcoma cell invasion and suppressed apoptosis under hypoxic condition [20].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Silence of lncRNA ANRIL represses cell growth and promotes apoptosis in retinoblastoma cells through regulating miR-99a and c-Myc

Wang

Zhang

Han

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Retinoblastoma is a rare cancer of the immature retina. This study designed to see the function of the lncRNA ANRIL in retinoblastoma Y79 cells. ANRIL, miR-99a and c-Myc expression in Y79 cells was altered by transfection and then trypan blue, transwell assay and flow cytometry were carried out to evaluate the changes of cell phenotype. The connection between ANRIL, miR-99a and c-Myc was measured by luciferase reporter assay and RNA immunoprecipitation analysis. As a result, ANRIL expression was highly expressed in human retinoblastoma tissue as relative to the adjacent noncancerous tissues. ANRIL suppression inhibited Y79 cells viability, migration, invasion, while promoted apoptosis. ANRIL negatively regulated miR-99a by binding to miR-99a. Silence of miR-99a reversed the ANRIL-knockdown effects on Y79 cells. miR-99a overexpression suppressed Y79 cell viability, migration, invasion, and enhanced apoptosis through downregulating c-Myc. Meanwhile, we found that miR-99a inhibited JAK/ STAT and PI3K/AKT pathways. To conclude, it seems that ANRIL suppression inhibits cell growth and metastasis in retinoblastoma Y79 cells by regulating miR-99a and c-Myc.

show abstract

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Silence of lncRNA ANRIL represses cell growth and promotes apoptosis in retinoblastoma cells through regulating miR-99a and c-Myc

Wang

Zhang

Han

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

show abstract

“…7,8 A number of lncRNAs have emerged as crucial regulators in the physiopathology of LSCC, specifically, as potential diagnostic biomarkers or therapeutic targets for this disease. 9,10 CDKN2B antisense RNA 1 (CDKN2B-AS1 also named ANRIL), a 3.8-kb lncRNA, was reported to be upregulated in tumor tissues and function as an oncogenic lncRNA in multiple cancers, such as hepatocellular carcinoma, 11 non-small cell lung cancer, 12 ovarian cancer, 13 cervical cancer, 14 pancreatic cancer, 15 and bladder cancer. 16 However, the biological function and underlying mechanism of CDKN2B-AS1 in LSCC remain unclear.…”

Section: Introductionmentioning

confidence: 99%

<p>Long Non-Coding RNA CDKN2B-AS1 Facilitates Laryngeal Squamous Cell Cancer Through Regulating miR-497/CDK6 Pathway</p>

Cui¹,

Yu²,

Shang³

et al. 2019

OTT

View full text Add to dashboard Cite

Background: CDKN2B antisense RNA 1 (CDKN2B-AS1), a long noncoding RNA, was reported to play crucial roles in the progression of multiple cancers. However, the functional roles and regulatory mechanism of CDKN2B-AS1 in human laryngeal squamous cell cancer (LSCC) remain unclear. The goals of this study were to investigate biological roles and underlying mechanisms of CDKN2B-AS1 in LSCC. Methods: The expressions of CDKN2B-AS1, miR-497 and cyclin-dependent kinase 6 (CDK6) were detected in LSCC tissues and cell lines by real-time quantitative PCR (qRT-PCR). The effects of CDKN2B-AS1 on LSCC cell proliferation, apoptosis, migration and invasion were examined by corresponding experiments. Bioinformatics analysis and luciferase activity assay were applied to analyze the interaction between CDKN2B-AS1 and miR-497. Results: The expression of CDKN2B-AS1 was significantly higher in LSCC tissues than in adjacent normal tissues. Higher CDKN2B-AS1 was closely associated with lymph node metastasis and advanced clinical stage. Moreover, CDKN2B-AS1 knockdown by siRNA significantly inhibited the proliferation, induced cell apoptosis, and suppressed migration and invasion in LSCC cells. Mechanically, CDKN2B functions as an oncogenic lncRNA in LSCC via regulating miR-497/CDK6 axis. Conclusion: The observations in this study identify CDKN2B-AS1 an oncogenic role in the tumorigenesis of LSCC by regulating miR-497/CDK6 axis and indicate that it may serve as a potential target for LSCC treatment.

show abstract

“…a recently published study reported that the expression of anril in the pancreatic cancer cells was higher than that in normal pancreatic duct epithelial cells and after anril is silenced, the migratory and invasive abilities of the cells were decreased (22). in the present study, Transwell and scratch-wound assays were used to examine the effects of anril on cell migration.…”

Section: Discussionmentioning

confidence: 81%

Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells

Wang

Zhang

2019

Mol Med Report

View full text Add to dashboard Cite

Pulmonary arterial hypertension (PaH) is a progressive syndrome. When PaH occurs, the circulatory resistance of the pulmonary vasculature will gradually increase, which may lead to right heart failure and death. Pathological features of PaH include abnormal proliferation of pulmonary vascular smooth muscle cells and pulmonary vascular remodeling. Hypoxia is the main cause of PaH, which directly induces the contraction and proliferation of pulmonary artery smooth muscle cells (PaSMcs), and eventually leads to pulmonary vascular remodeling. recent studies have shown that long non-coding rnas (lncrnas) play key roles in numerous biological processes, including cell proliferation and the occurrence and development of cardiovascular diseases. Studies have also shown that lncrna antisense noncoding rna in the inK4 locus (anril) can promote the proliferation of vascular smooth muscle cells. Therefore, the hypothesis of the present study was that anril may be expressed in PaSMcs and play a regulatory role. in this study, the expression of anril was analyzed by quantitative Pcr. The effects of anril on human pulmonary artery smooth muscle cells (HPaSMcs) were assessed by MTT assay, flow cytometry, bromodeoxyuridine incorporation assay, Transwell assay, scratch-wound assay, immunofluorescence assay and western blotting. These experiments revealed that the expression of ANRIL was significantly downregulated in HPaSMcs induced by hypoxia. The downregulation of anril affected the cell cycle, making more HPaSMcs move from the G0/G1 phase to the G2/M+S phase and strengthening the cell proliferation. Moreover, downregulated anril increased the migration of HPaSMcs under hypoxia. This study identified ANRIL as a critical regulator in HPASMCs induced by hypoxia and demonstrated the potential of gene therapy and drug development for treating PaH.

show abstract

The over expression of long non-coding RNA ANRIL promotes epithelial-mesenchymal transition by activating the ATM-E2F1 signaling pathway in pancreatic cancer: An in vivo and in vitro study

Cited by 47 publications

References 29 publications

RETRACTED ARTICLE: Silence of lncRNA ANRIL represses cell growth and promotes apoptosis in retinoblastoma cells through regulating miR-99a and c-Myc

RETRACTED ARTICLE: Silence of lncRNA ANRIL represses cell growth and promotes apoptosis in retinoblastoma cells through regulating miR-99a and c-Myc

<p>Long Non-Coding RNA CDKN2B-AS1 Facilitates Laryngeal Squamous Cell Cancer Through Regulating miR-497/CDK6 Pathway</p>

Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells

Contact Info

Product

Resources

About