Antimicrobial activity of PVP coated silver nanoparticles synthesized by Lysinibacillus varians

Bhatia, Divya; Mittal, Ashwani; Kumar, Deepak

doi:10.1007/s13205-016-0514-7

Cited by 42 publications

(36 citation statements)

References 46 publications

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“…In comparison to other studies, our results show superior antimicrobial activity. Bhati et al [20] determined the MIC values of PVP coated AgNPs with particle size 10-30 nm (prepared by biological method) against S. aureus and P. aeruginosa as 46 µg/mL and against C. albicans as 23 µg/mL. Dey et al [49] evaluated the MIC value of PVP-AgNPs with particle size 54 nm against S. aureus as 67.41 µg/mL.…”

Section: Inhibition Of Microbial Growthmentioning

confidence: 99%

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Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications

et al. 2017

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Polyvinylpyrrolidone (PVP) is employed in several potential applications, relying of its special chemical and physical properties in addition to its low toxicity and biocompatibility. The aim of this work is to prepare polyvinylpyrrolidone-silver (PVP-Ag) nanocomposite with high inhibiting effect on the microbial growth and low cytotoxicity. In situ prepared small stable spherical silver nanoparticles, with narrow range particle size distribution, were obtained by easy, economical and rapid chemical reduction method. Silver ions were reduced to silver nanoparticles using low amount of sodium borohydride (NaBH4) as a strong reducing agent. PVP-Ag nanocomposite was prepared using PVP as a stabilizing and capping agent. Formation of the spherical silver nanoparticles with mean particle size 5 nm was confirmed by ultraviolet-visible spectroscopy, high resolution transmission electron microscopy, and dynamic light scattering. The inhibiting growth effect of the nanocomposite toward Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Pseudomonas aeruginosa), and yeast fungus (Candida albicans) were studied. The cytotoxicity of the nanocomposite against BJ1 normal skin fibroblast cell line was tested. Results of this work presented perfect antimicrobial activity of the PVP-Ag nanocomposite towards bacteria and fungi with low cytotoxicity, which may lead to promising applications in skin wound healing.

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Section: Inhibition Of Microbial Growthmentioning

confidence: 99%

“…A variety of preparation methods, namely physical [16], chemical [17], biosynthetic [18] and biological [19,20] approaches have been studied to prepare AgNPs. The solution and solid state AgNPs could be produced by photochemical synthesis [21], laser ablation [22], microwave treatment [23] and γ-irradiation [24].…”

Section: Introductionmentioning

confidence: 99%

Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications

et al. 2017

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“…Both Gram-positive and Gram-negative bacterial strains are found to have adverse impacts on human as well as on animal lives; demand new alternative approach [1]. Nanotechnology is a great field to deal with diverse aspects of eco-system [2,3]. Nanoparticles (NPs) of metal, metal oxide, polymer and carbonaceous materials offer a wide range of properties followed by applications which allowed researchers to cross the boundaries of specialized area studies than known prevalent one [4,5].…”

Section: Introductionmentioning

confidence: 99%

Sol–gel auto-combustion mediated cobalt ferrite nanoparticles: a potential material for antimicrobial applications

et al. 2019

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In the era of nanotechnology, nanoparticles (NPs) of metals and metal oxides/chalcogenides are widely been used in medical applications where antibiotic-resistant microorganisms become a serious threat to the human health. Cobalt ferrite (CoFe 2 O 4) NPs, synthesized by a simple and cost-effective sol-gel auto-combustion method are envisaged for in vitro antimicrobial activities against Gram-positive bacteria (Bacillus subtilis; Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli; Pseudomonas aeruginosa). The structure, morphology, elemental analyses and surface area of CoFe 2 O 4 NPs are initially screened. The antimicrobial efficiency of CoFe 2 O 4 NPs is found to be optimum against the Gram-negative bacteria Escherichia coli (15 mm). In addition, membrane leakage assays performed to evaluate the intracellular cytoplasmic leakage with CoFe 2 O 4 NPs demonstrate the ability to destroy the bacterial membrane integrity, confirming their antimicrobial potential.

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“…Since antiquity, silver has been used to prevent gonorrhoeal eye infections, to repair vaginal tears after childbirth and to avoid infection of surgical wounds (Lemire et al 2013;Bhatia et al 2016). Due to the large surface-area-tovolume ratio, a nano form of silver is catalytically more active than the bulk material.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal potential of silver nanoparticles synthesized using cell-free extract of Comamonas acidovorans: in vitro and in silico approaches

Rudakiya

Pawar²

2017

3 Biotech

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The need to overcome human threats from pathogenic microbes, development of nanomaterials have been provoked for a new generation of antimicrobials. In the present study, biosynthesis of silver nanoparticles (AgNPs) was acquired using Comamonas acidovorans extract within 72 h under static condition. Electron microscopy studies revealed that the size of AgNPs was ranging from 6-53 nm and had spherical, oval and irregular shapes with smooth surfaces. Prepared AgNPs interacted with proteins, carbohydrates and other aromatic molecules. Biosynthesized AgNPs were bactericidal, which significantly inhibited pathogenic microbes, i.e., Streptococcus pyogenes, Staphylococcus aureus and Escherichia coli. Higher concentrations of AgNPs (20 lg ml -1 ) inhibited 92-98% growth of all tested bacteria within 24 h. AgNPs-protein network studies carried out to recognize the protein interactions with AgNPs and to understand probable bactericidal mechanisms. AgNPs may penetrate into cell through membrane proteins and damage them by modifying amino acids. Due to AgNPs-protein interactions, dysfunctions in enzymes obstruct certain metabolic processes, which cause the bacteria to die eventually. In certain pathogenic microbes, cue and cus systems detoxify Ag ? ions, transport through transporter proteins and expel them to the extracellular space, which are mainly responsible for Ag resistance.

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Antimicrobial activity of PVP coated silver nanoparticles synthesized by Lysinibacillus varians

Cited by 42 publications

References 46 publications

Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications

Assessment of in situ-Prepared Polyvinylpyrrolidone-Silver Nanocomposite for Antimicrobial Applications

Sol–gel auto-combustion mediated cobalt ferrite nanoparticles: a potential material for antimicrobial applications

Bactericidal potential of silver nanoparticles synthesized using cell-free extract of Comamonas acidovorans: in vitro and in silico approaches

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