<i>N</i>-Methyl Mesoporphyrin IX as an Effective Probe for Monitoring Alzheimer’s Disease β-Amyloid Aggregation in Living Cells

Li, Meng; Zhao, Andong; Ren, Jinsong; Qu, Xiaogang

doi:10.1021/acschemneuro.6b00436

Cited by 24 publications

(20 citation statements)

References 41 publications

(60 reference statements)

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“…NMM was also applied in a live-cell imaging study. When added to the cells, a large Stokes shift and a red-shift emission were observed, both of which were higher than the emissions seen with a different class of ligand, thioflavin T (ThT) [83]. This could be due to the green fluorescence emission of ThT, which can easily coincide with the intrinsic fluorescence of the cell’s other components [83].…”

Section: Fluorescent Turn-on G-quadruplex Ligandmentioning

confidence: 99%

G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications

Umar

Chan

et al. 2019

Molecules

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Guanine (G)-quadruplexes (G4s) are unique nucleic acid structures that are formed by stacked G-tetrads in G-rich DNA or RNA sequences. G4s have been reported to play significant roles in various cellular events in both macro- and micro-organisms. The identification and characterization of G4s can help to understand their different biological roles and potential applications in diagnosis and therapy. In addition to biophysical and biochemical methods to interrogate G4 formation, G4 fluorescent turn-on ligands can be used to target and visualize G4 formation both in vitro and in cells. Here, we review several representative classes of G4 fluorescent turn-on ligands in terms of their interaction mechanism and application perspectives. Interestingly, G4 structures are commonly identified in DNA and RNA aptamers against targets that include proteins and small molecules, which can be utilized as G4 tools for diverse applications. We therefore also summarize the recent development of G4-containing aptamers and highlight their applications in biosensing, bioimaging, and therapy. Moreover, we discuss the current challenges and future perspectives of G4 fluorescent turn-on ligands and G4-containing aptamers.

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Section: Fluorescent Turn-on G-quadruplex Ligandmentioning

confidence: 99%

G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications

Umar

Chan

et al. 2019

Molecules

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“…[73] Probe 109 (N-methyl mesoporphyrin IX, NMM, Figure 13A-C), as an easily available anionic porphyrin, was widely used to selectively detect G-quadruplexes DNA with a fluorescent enhancement, while little was known on its detailed interaction with amyloid fibrils. [74] Li et al discovered its applications on Aβ plaques for the first time. [74] On the one hand, probe 109 could be used in monitoring Aβ fibril formation in vitro and detecting intracellular Aβ plaques efficiently, attributing to a distinct Stokes shift of 210 nm.…”

Section: Figure 12 A)mentioning

confidence: 99%

“…[ 74 ] Li et al discovered its applications on Aβ plaques for the first time. [ 74 ] On the one hand, probe 109 could be used in monitoring Aβ fibril formation in vitro and detecting intracellular Aβ plaques efficiently, attributing to a distinct Stokes shift of 210 nm. Aβ fibrils could raise the red fluorescence emission, while Aβ monomers showed no obvious response under the same conditions.…”

Section: Alzheimer's Diseasementioning

confidence: 99%

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Fluorescent Diagnostic Probes in Neurodegenerative Diseases

et al. 2020

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Both chronic and acute neurodegenerative diseases have been associated with high morbidity and mortality, along with the death of neurons in different areas of the brain, and there are few or no effective curative therapy options for treatment of patients. [3,4] These disorders are a major cause of concern for the health and quality of life in the aging society, since age is the greatest risk factor for neurodegenerative disease. [5] The World Health Organization has predicted that in next 20 years neurodegenerative disease will become the second most common cause of mortality after cardiovascular disease. [6] Neurodegenerative processes begin long before their clinical symptoms are evident, and evolve for years slowly and irreversibly. Hence, there is an urgent need to diagnose neurodegenerative disease as early as possible and to distinguish between different neurodegenerative disorders with shared and unique symptoms to facilitate decision making regarding choice of treatment. Timely diagnosis is important for the adoption of therapeutic modalities in clinical trials prior to moderate dementia exhibition, to appraise and apply antidotes to maximally preserve the cognitive functions or to slow down the course of these disorders. The ability to analyze and identify risk factors for cognitive deficit would represent an extraordinary advancement in the field of dementia. [5] The main modern diagnostic technologies for the early examination of neurodegenerative disease pathology, neuroimaging techniques, include radioligandsbased positron emission tomography (PET), 3D single-photon emission-computed tomography (SPECT), and structural magnetic resonance imaging (MRI). Although PET and SPECT are the most used imaging techniques for neurodegenerative diseases, their application is limited by high cost, involvement of hazardous radiolabeled compounds, need for sophisticated instruments, and application of complex data acquisition and analysis protocols. The MRI technique has relatively low spatial resolution as well as intrinsically poor sensitivity to distinguish morphological differences between disease biomarkers and the surrounding tissue. Recently, fluorescence diagnosis technology has become an attractive and potential alternative to diagnose and probe the progression of neurodegenerative diseases, because of being rapid, noninvasive, sensitive, simple, real-time, low-cost, and high-resolution in nature. [7] There are several uses for Neurodegenerative diseases are debilitating disorders that feature progressive and selective loss of function or structure of anatomically or physiologically associated neuronal systems. Both chronic and acute neurodegenerative diseases are associated with high morbidity and mortality along with the death of neurons in different areas of the brain; moreover, there are few or no effective curative therapy options for treating these disorders. There is an urgent need to diagnose neurodegenerative disease as early as possible, and to distinguish between different disorders with overlap...

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“…Aβ is considered as the most toxic substance in the brain of AD patients (6). Thioflavin staining is the most suitable and standardized method for screening Aβ (7). Thioflavin-S is used for detecting Aβ plaques that can bind to distinctive β-pleated sheet of amyloid fibrils (8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Aβ Deposits in the Hippocampus of a Rat Model of Alzheimer’s Disease After Intravenous Injection of Human Adipose Derived Stem Cells by Immuno- and Thioflavin S-Costaining

Doshmanziari

Sarveazad

Moradi

et al. 2019

Thrita

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Background: Alzheimer's disease (AD) is a progressive neuropsychiatric disorder that gradually impairs memory and behavioral functions. Amyloid beta (Aβ) is considered as the most toxic substance in the brain of AD patients. Objectives: The present study was designed to evaluate Aβ deposits by Immuno-and Thioflavin S-costaining in the hippocampus of a rat model of AD after intravenous injection of human adipose-derived stem cells (hADSCs). Methods: Thirty-two male rats were included in the four groups of control, sham, AD and hADSCs. The hADSCs characterization was confirmed by the flow cytometry technique. Immuno-and Thioflavin S-costaining was utilized for detecting Aβ plaques in the hippocampus of a rat model of AD following injection of hADSCs. Results: Statistical analysis revealed that Aβ plaques increased significantly in the AD group compared to the control and sham groups. The administration of hADSCs significantly decreased immunoreactivity and Thio-S-positive plaques in the AD group. We also found that the plaques detected by anti-Aβ antibody (immunohistochemical staining) were significantly more than those distinguished by Thioflavin-S in all the groups. Conclusions: Results showed that hADSCs played an effective role in decreasing amyloids aggregation following migration to the hippocampus of the rat model of AD.

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N-Methyl Mesoporphyrin IX as an Effective Probe for Monitoring Alzheimer’s Disease β-Amyloid Aggregation in Living Cells

Cited by 24 publications

References 41 publications

G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications

G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications

Fluorescent Diagnostic Probes in Neurodegenerative Diseases

Evaluation of Aβ Deposits in the Hippocampus of a Rat Model of Alzheimer’s Disease After Intravenous Injection of Human Adipose Derived Stem Cells by Immuno- and Thioflavin S-Costaining

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