Characterization of Maillard-type lysozyme-galactomannan conjugate having immune-enhancing effects

Yang, Jae Eon; Chun, Su Hyun; Kim, Ha Hyung; Choi, Hee Don; Lee, Kwang Won

doi:10.1016/j.foodchem.2017.01.076

Cited by 11 publications

(4 citation statements)

References 41 publications

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“…baumannii and MRSA. In the present study, we confirmed that LYZOX was an MRP on the basis of increases in fluorescence intensity and molecular weight during SDS-PAGE, which were similar to previously reported results [16, 20]. Then, we assessed the antibacterial activity of LYZOX.…”

Section: Discussionsupporting

confidence: 92%

“…Maillard reaction products (MRPs) have fluorescence properties. Their fluorescence intensity increases during the Maillard reaction [20]. The fluorescence intensity of LYZOX (500 μg/mL) and a mixture of lysozyme (250 μg/mL) and COS (250 μg/mL) were measured in triplicate at an excitation wavelength of 370 nm and an emission wavelength of 440 nm using a fluorescence spectrometer (RF-6000, Shimadzu, Kyoto, Japan).…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial activity of lysozyme-chitosan oligosaccharide conjugates (LYZOX) against Pseudomonas aeruginosa, Acinetobacter baumannii and Methicillin-resistant Staphylococcus aureus

et al. 2019

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The recent emergence of antibiotic-resistant bacteria requires the development of new antibiotics or new agents capable of enhancing antibiotic activity. This study evaluated the antibacterial activity of lysozyme-chitosan oligosaccharide conjugates (LYZOX) against Pseudomonas aeruginosa , Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus (MRSA), which should resolve the problem of antibiotic-resistant bacteria. Bactericidal tests showed that LYZOX killed 50% more P . aeruginosa (NBRC 13275), A . baumannii and MRSA than the control treatment after 60 min. In addition, LYZOX was shown to inhibit the growth of P . aeruginosa (NBRC 13275 and PAO1), A . baumannii and MRSA better than its components. To elucidate the antibacterial mechanism of LYZOX, we performed cell membrane integrity assays, N-phenyl-1-naphthylamine assays, 2-nitrophenyl β-D-galactopyranoside assays and confocal laser scanning microscopy. These results showed that LYZOX affected bacterial cell walls and increased the permeability of the outer membrane and the plasma membrane. Furthermore, each type of bacteria treated with LYZOX was observed by electron microscopy. Electron micrographs revealed that these bacteria had the morphological features of both lysozyme-treated and chitosan oligosaccharide-treated bacteria and that LYZOX destroyed bacterial cell walls, which caused the release of intracellular contents from cells. An acquired drug resistance test revealed that these bacteria were not able to acquire resistance to LYZOX. The hemolytic toxicity test demonstrated the low hemolytic activity of LYZOX. In conclusion, LYZOX exhibited antibacterial activity and low drug resistance in the presence of P . aeruginosa , A . baumannii and MRSA and showed low hemolytic toxicity. LYZOX affected bacterial membranes, leading to membrane disruption and the release of intracellular contents and consequent bacterial cell death. LYZOX may serve as a novel candidate drug that could be used for the control of refractory infections.

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Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Antibacterial activity of lysozyme-chitosan oligosaccharide conjugates (LYZOX) against Pseudomonas aeruginosa, Acinetobacter baumannii and Methicillin-resistant Staphylococcus aureus

et al. 2019

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“…The previous studies have also confirmed that Maillard-type protein-polysaccharide conjugates, such as lysozyme-galactomannan conjugates, can increase the expressions of cytokines produced by murine macrophages. 67,68 In addition, inflammation is the response of immune system to harmful stimulation. 69 Maillard-type glycine-glucose conjugates and Maillard-type whey protein isolate-glucose conjugates can modulate inflammatory responses by regulating the NF-κB signaling pathway and JAK/ STAT signaling pathway in human macrophages.…”

Section: Food and Function Papermentioning

confidence: 99%

Oat protein isolate-Pleurotus ostreatus β-glucan conjugate nanoparticles bound to β-carotene effectively alleviate immunosuppression by regulating gut microbiota

Zhong,

Hu,

Zhan

et al. 2024

Food Funct.

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“…In addition to dextran, antioxidant activity of ovalbumin [32,33], antioxidant and antimicrobial activity of lysozyme [33,34], and emulsifying and antioxidant properties of phosvitin [25,35] were all significantly enhanced through conjugation reactions of the proteins with galactomannans, a group of polysaccharides derived from the cell walls of leguminous plants and Aspergillus spp. fungi [36].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Properties of Casein Phosphopeptides (CPP) and Maillard-Type Conjugated Products

2020

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A casein phosphopeptide (CPP) fraction derived from tryptic hydrolysis of bovine casein was evaluated for antioxidant activity. Conjugations or mixtures of CPP with polysaccharide, galactomannan (Gal), or xyloglucan (Xyl) were prepared to evaluate potential enhancement of CPP antioxidant activity. The effect of calcium was also investigated. The CPP preparation alone was effective at scavenging hydroxyl radicals and sequestering Fe2+ to protect against Fenton reaction-induced deoxyribose oxidation in non-site-specific (up 63.3% inhibition) and site-specific (up 32.1% inhibition) binding assays, respectively. CPP also effectively quenched 2,2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radicals (ABTS•+) to an extent of 67.6% scavenging in an aqueous system. In a soybean lecithin liposome system, CPP exhibited effective protection against peroxyl radical-induced liposomal peroxidation (38.3% of control in terms of rate of propagation). Conjugating CPP with Gal or Xyl polysaccharides using Maillard reaction conditions significantly reduced activity in the Fenton reaction-deoxyribose assays, while exhibiting no effect on the antioxidant activity of native CPP in both the ABTS and liposome assays, respectively. These results represent comparative antioxidant capacity of the native CPP and associated conjugates in phases that varied in relative hydrophilic and hydrophobic character. We conclude that CPP has the potential to act as both a primary and secondary antioxidant by displaying transition metal ion sequestering activity and free radical quenching activity. Improvements in antioxidant activity of CPP by Maillard-type conjugation with Xyl or Gal were relatively small and model-specific.

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Characterization of Maillard-type lysozyme-galactomannan conjugate having immune-enhancing effects

Cited by 11 publications

References 41 publications

Antibacterial activity of lysozyme-chitosan oligosaccharide conjugates (LYZOX) against Pseudomonas aeruginosa, Acinetobacter baumannii and Methicillin-resistant Staphylococcus aureus

Antibacterial activity of lysozyme-chitosan oligosaccharide conjugates (LYZOX) against Pseudomonas aeruginosa, Acinetobacter baumannii and Methicillin-resistant Staphylococcus aureus

Oat protein isolate-Pleurotus ostreatus β-glucan conjugate nanoparticles bound to β-carotene effectively alleviate immunosuppression by regulating gut microbiota

Antioxidant Properties of Casein Phosphopeptides (CPP) and Maillard-Type Conjugated Products

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