Hepatoprotective effect of Maytenus robusta Reiss extract on CCl4-induced hepatotoxicity in mice and HepG2 cells

Thiesen, Liliani Carolini; Silva, Luísa Mota da; Santin, José Roberto; Bresolin, Tania Mari Bellé; Andrade, Sérgio Faloni de; Amorim, Clarissa de Medeiros; Merlin, L.; Freitas, Rilton Alves de; Niero, Rivaldo; Netz, Daisy J. A.

doi:10.1016/j.yrtph.2017.02.023

Cited by 11 publications

(4 citation statements)

References 25 publications

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“…Extract reduced the hepatic histological damages and normalize hepatic biomarkerss. The antioxidant effect of M. robusta in liver tissue promoted the reduction in lipoperoxides levels increased the reduced glutathione content and increased the activity of superoxide dismutase, catalase, and glutathione-S-transferase Moreover, the extract reduced hepatic inflammation by diminishing myeloperoxidase activity, TNF and interleukin-6 levels by its antioxidant effects [14]. Similarly, Maytenus emarginata ethanol extract possess potent hepatoprotective effect.…”

Section: Introductionmentioning

confidence: 95%

Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice

et al. 2020

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Background: Myrin®-p Forte is an anti-tuberclosis agent that can cause hepatic injuries in clinical settings. Maytenus royleanus (Celastraceae) is a medicinal plant, possesses antioxidant and anticancer activities. The hepatoprotective effect of the methanol extract of Maytenus royleanus leaves (MEM) against Myrin®-p Forte induced hepatotoxicity in mice was investigated.Methods: Mice were randomly parted into six groups (n = 6). Fixed-dose combination of Myrin®-p Forte (13.5 mg/ kg Rifampicin, 6.75 mg/kg Isoniazid, 36.0 mg/kg Pyrazinamide and 24.8 mg/kg Ethambutol; RIPE] was administered for 15 days to induce liver injury. In treatment groups MEM (200 mg/kg and 400 mg/kg doses) and Vitamin B6 (180mg/kg) were administered prior to RIPE. Control group received 2% DMSO. Serum liver function tests, DNA damage, tissue antioxidant enzymes and histopathological alterations were studied. HPLC analysis was performed to determine the chemical composition using standard compounds.(Continued on next page) Results: The quercitin, gallic acid, luteolin, viteixin, apigenin, kaempherol, hyperoside and myricetin contents of all samples were determined by reverse-phase HPLC. Quercetin (0.217 mg/g dry weight) and luteolin (0.141 mg/g dry weight) were the major flavonoids identified in MEM. Myrin®-p Forte markedly (p < 0.05) deteriorated lipid profile and upregulated the concentration of LDH, AST, ALP, ALT and γ-GT in serum along with DNA fragmentation (37.13 ± 0.47%) and histopathological injuries in hepatic tissues of mice compared with the control group. Myrin®-p Forte increased (p < 0.001) lipid peroxidation and H 2 O 2 while decreased (p < 0.001) the activity level of CAT, SOD, POD, GPx, GST, GSR, γ-GT and GSH. Co-administration of MEM (200 mg/kg; 400 mg/kg) or the vitamin B6 (180 mg/ kg) to Myrin®-p Forte administered mice significantly ameliorated LDL, cholesterol, HDL and triglyceride content. Furthermore, MEM dose dependently corrected serum liver function tests, decrease % DNA fragmentation (17.82 ± 0.35 and 7.21 ± 0.32 respectively), DNA damage. MEM treated protect RIPE induced oxidative damage by enhancing antioxidants to oxidants balance. Histological examination comprehends biochemical findings. Conclusion: The antioxidant effects of MEM exerted the hepatoprotective potential against the Myrin®-p Forte induced hepatotoxicity in mice.

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Section: Introductionmentioning

confidence: 95%

Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice

et al. 2020

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“…Medicinal plants have been reported to possess hepatoprotective effects against liver toxicity. For example, Thiesen et al (2017) evaluated the hepatoprotective effect of the methanolic extract (300 μg/mL) from Maytenus robusta Reissek leaves in carbon tetrachloride (CCl 4 )-induced hepatotoxicity in HepG2 cells. The extract was observed to restore cell viability from 29.56% to 65.27%.…”

Section: Discussionmentioning

confidence: 99%

Flavonoids of Chromolaena odorata (L.) R.M.King & H.Rob. as potential leads for treatment against tuberculosis

Omokhua-Uyi

Madikizela

Aro

et al. 2023

South African Journal of Botany

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“…Several extracts from natural plants such as Maytenus robusta Reiss [19], Phyllanthus amarus [20], Fagonia schweinfurthii Hadidi [21], and Herpetospermum caudigerum [22] were shown to protect the liver from oxidant damage induced with CCl4. In our study, we showed that administration of R. sativus significantly decreased the activities of the oxidants, MDA, and GSH, while increasing the activities of antioxidant enzymes, SOD and CAT in CCl4induced hepatotoxicity in rats.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical analysis and hepatoprotective activity of Raphanus sativus var. sativus in Sprague-Dawley rats

Rahman¹,

Bayz²,

Hussein³

et al. 2020

Trop. J. Pharm Res

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Purpose: To determine the phenolic and flavonoid contents of R. sativus rhizome ethanol extract and the hepatoprotective effect of the extract in rats. Methods: Folin–Ciocalteau and aluminum chloride colorimetric tests were used to determine the contents of phenols and flavonoids in the R. sativus extract. Male Sprague-Dawley rats induced with CCl4 to develop hepatotoxicity were treated orally with R. sativus extract for 4 weeks. The antioxidant and anti-inflammatory effects of the extract on the liver were determined by evaluating the concentration of oxidative analytes, serum liver enzymes and lipids, and hepatic histopathology and cytochrome P450 2E1 expression. Results: R. sativus extract significantly (p < 0.05) reduced the hepatotoxic effect of CCl4 via its antioxidant activities and protection of liver tissues from oxidative damage. Conclusion: The hepatoprotective effects of R. sativus rhizome ethanol extract are attributed to its highphenolic and flavonoid contents. Keywords: R. savitus rhizome, Phenols, Flavonoid contents, antioxidant, Hepatoprotective

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Hepatoprotective effect of Maytenus robusta Reiss extract on CCl4-induced hepatotoxicity in mice and HepG2 cells

Cited by 11 publications

References 25 publications

Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice

Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice

Flavonoids of Chromolaena odorata (L.) R.M.King & H.Rob. as potential leads for treatment against tuberculosis

Phytochemical analysis and hepatoprotective activity of Raphanus sativus var. sativus in Sprague-Dawley rats

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