Extra and intracellular calcium signaling pathway(s) differentially regulate histamine-induced myometrial contractions during early and mid-pregnancy stages in buffaloes ( Bubalus bubalis )

Sharma, Abhıshek; Nakade, Udayraj P.; Choudhury, Soumen; Yadav, Rajkumar Singh; Garg, Satish K.

doi:10.1016/j.anireprosci.2017.01.011

Cited by 5 publications

(2 citation statements)

References 46 publications

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“…This moderate Ca 2+ release induces the opening of the calcium channels thus causing an increase in the calcium ow at the origin of the contractions. These results are similar to those of Sharma et al [37] who showed that histamine acts by rst mobilizing calcium reserves and extracellular calcium.…”

Section: Discussionsupporting

confidence: 91%

Uterotonic Effects of Aqueous and Methanolic Extracts of Lannea acida in Wistar Rats: An In Vitro Study

et al. 2021

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Background: Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (West-Cameroon), is a tree whose bark is used locally to solve di cult childbirth. This study aimed to evaluate the in vitro uterotonic effects of aqueous and methanolic extracts of L. acida in female Wistar rats. Uterine strips isolated from female rats pretreated (48h) with oestradiol (5μg) were mounted in a singleorgan bath containing a well aerated and thermostated De Jalon solution (37°C). The effects of L. acida extracts were recorded in a non-cumulative manner after application. The effect of the methanolic extract (the most active extract) was monitored in the presence of atosiban (a competitive antagonist of oxytocin receptors), atropine (a speci c type 3 muscarinic receptor antagonist), nifedipine (an L-type calcium channel antagonist) and 2-Aminoethoxydiphenyl borate (2-ADB, a speci c antagonist of inositol 1,4,5triphosphate receptors type 1), and in calcium-free medium containing EGTA.Results: L. acidainduced uterine contraction in a concentration-dependent manner with the methanolic extract (1.506 ± 0.032 gf) being the most effective. Administration of atosiban (2 μmol/l), atropine (1 μmol/l), nifedipine (5 μmol), 2-APB (100 μmol), and calcium free medium containing EGTA (2 mmol) reduced the contractile effect of L. acida. Complete inhibition was observed with nifedipine, 2-APB, and calcium free medium containing EGTA.Conclusions: These results suggest that L. acida possesses an uterotonic effect mediated through oxytocin receptors with mobilization of extracellular calcium.

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Section: Discussionsupporting

confidence: 91%

Uterotonic Effects of Aqueous and Methanolic Extracts of Lannea acida in Wistar Rats: An In Vitro Study

et al. 2021

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“…The arterial rings of 1.5–2 mm were then mounted between two fine stainless steel L-shaped hooks and kept under a resting tension of 1.5 g in a thermostatically controlled (37.0±0.5°C) 20 ml organ bath containing MKHS continuously aerated with Carbogen. Isometric contraction studies were performed as described previously with minor modifications (Anderson et al, 2014; Dash and Parija, 2013; Mohanty et al, 2016; Singh et al, 2016; Sharma et al, 2017). Briefly, the arterial rings were equilibrated for 90 min before recording of muscle tension, washed every 15 min (using MKHS freshly adjusted to pH 7.4 or 6.8) and the experiment repeated for both endothelium intact (ED+) and denuded (ED-) vessels wherever necessary.…”

Section: Methodsmentioning

confidence: 99%

Acidosis potentiates endothelium-dependent vasorelaxation and gap junction communication in the superior mesenteric artery

Mohanty

Parija

Suklabaidya

et al. 2018

European Journal of Pharmacology

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Extracellular pH is an important physiological determinant of vascular tone that is normally maintained within 7.35-7.45. Any change outside this range leads to severe pathological repercussions. We investigated the unknown effects of extracellular acidosis on relaxation in the superior mesenteric artery (SMA) of goat. SMA rings were employed to maintain isometric contractions at extracellular pH (pH) 7.4 and 6.8. We analyzed the effect of acidosis (pH 6.8) compared to physiological pH (pH 7.4) on three signaling mediators of endothelium-dependent hyperpolarization: nitric oxide (NO), prostaglandin I (PGI), and myoendothelial gap junctions (MEGJ). NO and cyclic guanosine monophosphate (cGMP) levels were compared between normal and acidic pH. Quantitative real-time PCR (qPCR) studies determined the change in expression of vascular connexin (Cx), Cx37, Cx40, and Cx43. Under acidosis, acetyl choline-induced relaxation was augmented in an endothelium-dependent manner via eNOS-NO-cGMP signaling. Conversely, at normal pH, acetyl choline-induced vasorelaxation was mediated primarily via COX-PGI pathway. The functional activity of MEGJ was increased under acidosis as evident from increased sensitivity of connexin blockers and upregulated gene and protein expression of connexins. In conclusion, acetyl choline-induced augmented vasorelaxation under acidosis is mediated by NOS-NO-cGMP, with a partial role of MEGJ as EDH mediators in the SMA. Present data suggest a novel role of connexin as therapeutic targets to attenuate the detrimental effect of acidosis on vascular tone.

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Calcium signaling cascades differentially regulate PGF2α-induced myometrial contractions in water buffaloes (Bubalus bubalis)

Sharma

Nakade

Raut

et al. 2021

Naunyn-Schmiedeberg's Arch Pharmacol

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Extra and intracellular calcium signaling pathway(s) differentially regulate histamine-induced myometrial contractions during early and mid-pregnancy stages in buffaloes ( Bubalus bubalis )

Cited by 5 publications

References 46 publications

Uterotonic Effects of Aqueous and Methanolic Extracts of Lannea acida in Wistar Rats: An In Vitro Study

Uterotonic Effects of Aqueous and Methanolic Extracts of Lannea acida in Wistar Rats: An In Vitro Study

Acidosis potentiates endothelium-dependent vasorelaxation and gap junction communication in the superior mesenteric artery

Calcium signaling cascades differentially regulate PGF2α-induced myometrial contractions in water buffaloes (Bubalus bubalis)

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