Bufadienolides from Kalanchoe daigremontiana as thrombin inhibitors— In vitro and in silico study

Kołodziejczyk-Czepas, Joanna; Sieradzka, Malgorzata; Moniuszko-Szajwaj, Barbara; Pecio, Łukasz; Ponczek, Michał B.; Nowak, Paweł; Stochmal, Anna

doi:10.1016/j.ijbiomac.2017.02.051

Cited by 21 publications

(11 citation statements)

References 31 publications

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“…Thus, the influence of Kalanchoe -derived bufadienolides on enzymatic properties of serine proteinases still is very poorly evidenced. Inhibitory action of bufadienolide-rich fraction from K. daigremontiana on enzymatic activity of thrombin has been recently described by Kolodziejczyk-Czepas et al ( 2017 ). Native (untreated with the examined fraction) thrombin was used as a control sample.…”

Section: Biological Activity Of Kalanchoe Species-mentioning

confidence: 90%

Bufadienolides of Kalanchoe species: an overview of chemical structure, biological activity and prospects for pharmacological use

Kołodziejczyk-Czepas

Stochmal

2017

Phytochem Rev

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Toad venom is regarded as the main source of bufadienolides; however, synthesis of these substances takes also place in a variety of other animal and plant organisms, including ethnomedicinal plants of the Kalanchoe genus. Chemically, bufadienolides are a group of polyhydroxy C-24 steroids and their glycosides, containing a six-membered lactone (α-pyrone) ring at the C-17β position. From the pharmacological point of view, bufadienolides might be a promising group of steroid hormones with cardioactive properties and anticancer activity. Most of the literature concerns bufadienolides of animal origin; however, the medicinal use of these compounds remains limited by their narrow therapeutic index and the risk of development of cardiotoxic effects. On the other hand, plants such as Kalanchoe are also a source of bufadienolides. Kalanchoe pinnata (life plant, air plant, cathedral bells), Kalanchoe daigremontiana (mother of thousands) and other Kalanchoe species are valuable herbs in traditional medicine of Asia and Africa. The present review focuses on the available data on chemical structures of 31 compounds, biological properties and prospects for therapeutic use of bufadienolides from Kalanchoe species. Furthermore, it presents some new investigational trends in research on curative uses of these substances.

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Section: Biological Activity Of Kalanchoe Species-mentioning

confidence: 90%

Bufadienolides of Kalanchoe species: an overview of chemical structure, biological activity and prospects for pharmacological use

Kołodziejczyk-Czepas

Stochmal

2017

Phytochem Rev

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“…For instance, the aqueous extracts of B. pinnatum have been shown to exhibit in vivo antihypertensive activity on high salt-loaded rats models [69]. Furthermore, isolates from B. daigremontianum root extracts developed an in vitro anti-thrombotic activity [70].…”

Section: Species Ethnobotanical Uses Plant Organ Locationsmentioning

confidence: 99%

From Ethnomedicine to Plant Biotechnology and Machine Learning: The Valorization of the Medicinal Plant Bryophyllum sp.

et al. 2020

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The subgenus Bryophyllum includes about 25 plant species native to Madagascar, and is widely used in traditional medicine worldwide. Different formulations from Bryophyllum have been employed for the treatment of several ailments, including infections, gynecological disorders, and chronic diseases, such as diabetes, neurological and neoplastic diseases. Two major families of secondary metabolites have been reported as responsible for these bioactivities: phenolic compounds and bufadienolides. These compounds are found in limited amounts in plants because they are biosynthesized in response to different biotic and abiotic stresses. Therefore, novel approaches should be undertaken with the aim of achieving the phytochemical valorization of Bryophyllum sp., allowing a sustainable production that prevents from a massive exploitation of wild plant resources. This review focuses on the study of phytoconstituents reported on Bryophyllum sp.; the application of plant tissue culture methodology as a reliable tool for the valorization of bioactive compounds; and the application of machine learning technology to model and optimize the full phytochemical potential of Bryophyllum sp. As a result, Bryophyllum species can be considered as a promising source of plant bioactive compounds, with enormous antioxidant and anticancer potential, which could be used for their large-scale biotechnological exploitation in cosmetic, food, and pharmaceutical industries.

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“…The members of the Kalanchoë genus include also several species with a broad range of ethnomedicinal uses. Therapeutic action of Kalanchoë plants is attributed to bufadienolides, a group of poly-hydroxy steroid hormones, displaying pharmacological activities such as anticancer, anti-inflammatory and cardioactive effects [37].…”

Section: Introductionmentioning

confidence: 99%

Phylogenomic Analysis of the PEBP Gene Family from Kalanchoë

et al. 2019

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The PEBP family comprises proteins that function as key regulators of flowering time throughout the plant kingdom and they also regulate growth and plant architecture. Within the PEBP protein family, three subfamilies can be distinguished in angiosperms: MOTHER OF FT AND TFL1-like (MFT), FLOWERING LOCUS T-like (FT-like), and TERMINAL FLOWER1-like (TFL1-like). Taking advantage of the genome sequences available from K. fedtschenkoi and K. laxiflora, we performed computational analysis to identify the members of the PEBP gene family in these species. The analyses revealed the existence of 11 PEBP genes in K. fedtschenkoi and 18 in K. laxiflora, which are clustered in two clades: FT-like and TFL1-like. The PEBP genes had conserved gene structure and the proteins had highly conserved amino acid sequences in the positions crucial for the protein functions. The analysis of Ka/Ks ratio revealed that most recently duplicated genes are under positive selection. Despite being an economically important genus, the genetics underlying the regulation of flowering in Kalanchoë is poorly understood. The results of this study may provide a new insight into the molecular control of flowering that will allow further studies on flowering control in Kalanchoë.

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Bufadienolides from Kalanchoe daigremontiana as thrombin inhibitors— In vitro and in silico study

Cited by 21 publications

References 31 publications

Bufadienolides of Kalanchoe species: an overview of chemical structure, biological activity and prospects for pharmacological use

Bufadienolides of Kalanchoe species: an overview of chemical structure, biological activity and prospects for pharmacological use

From Ethnomedicine to Plant Biotechnology and Machine Learning: The Valorization of the Medicinal Plant Bryophyllum sp.

Phylogenomic Analysis of the PEBP Gene Family from Kalanchoë

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