Characterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China

Abstract

BackgroundThe implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria.Methods Staphylococcus phage JD007 was characterized and its complete genome sequence analysed.Results Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5–11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest Staphylococcus phages of Myoviridae. No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was able to lyse 95% of S. aureus isolates, including the prevalent ST239-MRSA and ST59-MRSA strains isolated from different hospitals in Shanghai, China, and inhibition assays showed that JD007 could inhibit S. aureus growth at a multiplicity of infection of 0.1.ConclusionsThe results suggested that Staphylococcus phage JD007 can potentially be used in phage therapy or for the detection of S. aureus.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-017-0701-0) contains supplementary material, which is available to authorized users.

A Phage Lysin Fused to a Cell-Penetrating Peptide Kills Intracellular Methicillin-Resistant Staphylococcus aureus in Keratinocytes and Has Potential as a Treatment for Skin Infections in Mice

“…Characterization of staphylococcal lysin derived from phage JD007. A previous study demonstrated that phage JD007 exhibits strong antibacterial activity against various types of S. aureus strains (25). In this study, a lysin was identified from the whole-genome sequence of phage JD007 (JDlys).…”

“…Escherichia coli strains, used for gene cloning and producing recombinant proteins, were cultured aerobically in Luria-Bertani (LB) broth with shaking (180 rpm) at 37°C. The JD007 phage was kindly provided by ZeLin Cui (Shanghai General Hospital, Shanghai, China) (25). Purification and genomic DNA extraction were performed as described previously (13).…”

Metagenome analysis of Russian and Georgian Pyophage cocktails and a placebo‐controlled safety trial of single phage versus phage cocktail in healthy Staphylococcus aureus carriers

“…These variant Kayviruses represent thus interesting starting material to address different molecular steps of the phage-host interaction process. The maintenance of a Kayvirus in all commercial products from commercial Eastern phage cocktails is also explained by the exceptionally broad host range of this phage, which achieves, 60% to >95% coverage when tested against different collections of S. aureus clinical isolates (Hsieh et al, 2011;Alves et al, 2014;Cui et al, 2017). Phage K, the prototype of Kayviruses, binds to the highly-conserved wall-teichoic acids (WTA) on the surface of the S. aureus cell wall, which explains its broad host range (O'Flaherty et al, 2005;Xia et al, 2011).…”

Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Endotracheal Tubes

“…To evaluate thermal stability, 100 μl of the bacteriophage suspension (10 8 PFU/ml) was added to 900 μl of elution buffer and incubated at different temperatures (37, 42, 50, 60, 70, and 80°C) for 2 h (20). After the incubation period, the phage titer was determined through the double-layer agar method ( Cui et al, 2017 ). The assays were carried out at three independent times in triplicate.…”