Microbial control of arthropod-borne disease

Saldaña, Miguel A.; Hegde, Shivanand; Hughes, Grant L.

doi:10.1590/0074-02760160373

Cited by 64 publications

(70 citation statements)

References 211 publications

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“…There is a complex interplay between the host innate immune system and microbiota which maintains microbiome homeostasis [16, 62, 63]. However, invading arboviral pathogens are also susceptible to these immune pathways [64, 65] thereby providing an indirect mechanism by which microbiota can interfere with pathogens.…”

Section: Resultsmentioning

confidence: 99%

“…While our understanding of genetic factors that influence host-microbe interactions and microbiome acquisition are expanding [10, 11], we still have a poor knowledge of these interactions at the cellular level. Given the importance of the microbiome on mosquito traits relevant for vectorial capacity and vector competence [12–15], understanding processes that influence microbiome homeostasis is critical for developing microbial-based control strategies [16, 17].…”

Section: Introductionmentioning

confidence: 99%

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Gut-associated bacteria invade the midgut epithelium of Aedes aegypti and stimulate innate immunity and suppress Zika virus infection in cells

Hegde

Voronin

Casas-Sánchez

et al. 2019

Preprint

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23Microbiota within mosquitoes influence nutrition, immunity, fecundity, and the capacity 24 to transmit pathogens. Despite their importance, we have a limited understanding of 25 host-microbiota interactions, especially at the cellular level. It is evident bacterial 26 symbionts that are localized within the midgut also infect other organs within the 27 mosquito; however, the route these symbionts take to colonize other tissues is 28 unknown. Here, utilizing the gentamicin protection assay, we showed that the bacterial 29 symbionts Cedecea and Serratia have the capacity to invade and reside intracellularly 30 within mosquito cells. Symbiotic bacteria were found within a vacuole and bacterial 31 replication was observed in mosquito cell by transmission electron microscopy, 32indicating bacteria were adapted to the intracellular milieu. Using gene silencing, we 33 determined that bacteria exploited host factors, including actin and integrin receptors, to 34 actively invade mosquito cells. As microbiota can affect pathogens within mosquitoes, 35we examined the influence of intracellular symbionts on Zika virus (ZIKV) infection. 36Mosquito cells harbouring intracellular bacteria had significantly less ZIKV compared to 37 uninfected cells or cells exposed to non-invasive bacteria. Intracellular bacteria were 38 observed to substantially upregulate the Toll and IMD innate immune pathways, 39 providing a possible mechanism mediating these anti-viral effects. Examining mono-40 axenically infected mosquitoes using transmission electron and fluorescent microscopy 41 revealed that bacteria occupied an intracellular niche in vivo. Our results provided 42 evidence that bacteria that associate with the midgut of mosquitoes have intracellular 43 lifestyles which likely have implications for mosquito biology and pathogen infection. 44 This study expands our understanding of host-microbiota interactions in mosquitoes, 45 3 which is important as symbiont microbes are being exploited for vector control 46 strategies. 47 48 4 Introduction 49

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gut-associated bacteria invade the midgut epithelium of Aedes aegypti and stimulate innate immunity and suppress Zika virus infection in cells

Hegde

Voronin

Casas-Sánchez

et al. 2019

Preprint

Self Cite

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“…Temperature variation in general could profoundly impact arbovirus infection and replication early in infection due to shifts in the balance and dynamics of the midgut environment, the first host environment encountered. This environment is fairly complex as arboviruses encounter microbiota (Xi et al, 2008; Carissimo et al, 2014; Hegde et al, 2015; Saraiva et al, 2016; Barletta et al, 2017; Saldaña et al, 2017b), oxidative and nitration stress associated with digestion of the blood meal (Luckhart et al, 1998; Graça-Souza et al, 2006; Xi et al, 2008), and key immune factors (Campbell et al, 2008; Xi et al, 2008; Cirimotich et al, 2009; Sánchez-Vargas et al, 2009; Souza-Neto et al, 2009). To better understand the effects of temperature on the ZIKV-mosquito interaction, we used RNA sequencing to describe the transcriptional response of Ae.…”

Section: Introductionmentioning

confidence: 99%

Temperature Dramatically Shapes Mosquito Gene Expression with Consequences for Mosquito-Zika Virus Interactions

Ferreira

Tesla

aacutecio

et al. 2019

Preprint

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5Vector-borne flaviviruses are emerging threats to human health. For successful 3 6 transmission, the virus needs to efficiently enter mosquito cells, replicate within, and 3 7 escape several tissue barriers while mosquitoes elicit major transcriptional responses to 3 8 flavivirus infection. This process will not only be affected by the specific mosquito-3 9 pathogen pairing, but also variation in key environmental variables such as temperature. 4 0 Thus far, few studies have examined the molecular responses triggered by temperature 4 1 and how these responses modify infection outcomes despite substantial evidence 4 2 showing strong relationships between temperature and transmission in a diversity of 4 3 systems. To define the host transcriptional changes associated with temperature 4 4 variation during the early infection process, we compared the transcriptome of mosquito 4 5midgut samples from mosquitoes exposed to Zika virus (ZIKV) and non-exposed 4 6 mosquitoes housed at three different temperatures (20, 28, and 36°C). While the high 4 7 temperature samples did not have significant changes from standard rearing conditions 4 8 These temperature constraints on infection are likely regulated through different 1 3 2 mechanisms. Temperature variation in general could profoundly impact arbovirus

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“…These genetically modified bacteria cause harmful effects in the host body, dysregulate its sexual cycle, decrease the host competence and interfere with the developmental processes of the vector species, thereby suppressing the vector population (Wilke and Marrelli, 2015). As reported in the study by Jeffery et al, the most effective bacterial agents used is Wolbachia (Jeffery et al, 2009; Saldaña et al, 2017), which is a reproductive parasite interfering with the cellular and reproductive mechanisms of the vector species (Araújo et al, 2015; Kamtchum-Tatuene et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Prevention and Control Strategies to Counter Dengue Virus Infection

Rather

Parray

Lone

et al. 2017

Front. Cell. Infect. Microbiol.

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Dengue is currently the highest and rapidly spreading vector-borne viral disease, which can lead to mortality in its severe form. The globally endemic dengue poses as a public health and economic challenge that has been attempted to suppress though application of various prevention and control techniques. Therefore, broad spectrum techniques, that are efficient, cost-effective, and environmentally sustainable, are proposed and practiced in dengue-endemic regions. The development of vaccines and immunotherapies have introduced a new dimension for effective dengue control and prevention. Thus, the present study focuses on the preventive and control strategies that are currently employed to counter dengue. While traditional control strategies bring temporary sustainability alone, implementation of novel biotechnological interventions, such as sterile insect technique, paratransgenesis, and production of genetically modified vectors, has improved the efficacy of the traditional strategies. Although a large-scale vector control strategy can be limited, innovative vaccine candidates have provided evidence for promising dengue prevention measures. The use of tetravalent dengue vaccine (CYD-TDV) has been the most effective so far in treating dengue infections. Nonetheless, challenges and limitation hinder the progress of developing integrated intervention methods and vaccines; while the improvement in the latest techniques and vaccine formulation continues, one can hope for a future without the threat of dengue virus.

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Microbial control of arthropod-borne disease

Cited by 64 publications

References 211 publications

Gut-associated bacteria invade the midgut epithelium of Aedes aegypti and stimulate innate immunity and suppress Zika virus infection in cells

Gut-associated bacteria invade the midgut epithelium of Aedes aegypti and stimulate innate immunity and suppress Zika virus infection in cells

Temperature Dramatically Shapes Mosquito Gene Expression with Consequences for Mosquito-Zika Virus Interactions

Prevention and Control Strategies to Counter Dengue Virus Infection

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