The Optimization of Electrophoresis on a Glass Microfluidic Chip and its Application in Forensic Science

Han, Jun P.; Sun, Jing; Wang, Le; Liu, Peng; Zhuang, Bin; Zhao, Lei; Liu, Yao; Li, Cai X.

doi:10.1111/1556-4029.13408

Cited by 16 publications

(17 citation statements)

References 35 publications

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“…The use of handheld 3-D laser scanners [60,61]and Near-infrared (Near IR) light scanners [62] can also help scientist/police to identify a potential suspect during an investigation. The microfluidic chips on the other hand allow scientists to reduce contamination and build DNA profile at the scene of crime [5][6][7]63]. Portable forensics labs could also be equipped with devices capable of transmitting data obtained through the use of next generation facial recognition software and fingerprint scans to government databases for comparison with stored files of such information.…”

Section: Conclusive Remarks and Future Prospectivementioning

confidence: 99%

“…Following refinement and rigorous testing, many methods/technologies have been adopted by forensic laboratories, including polymerase chain reaction (PCR) [1,2], capillary electrophoretic instrumentation (Genetic Analyzers) [3,4], automated liquid handling (Microfluidic devices) [5][6][7], and expert software systems [8][9][10][11]. Introduction of robust validation processes has a long term impact to test evidentiary samples to be presented to the court of law.…”

Section: Introductionmentioning

confidence: 99%

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The Future of Forensic Biology

Rana¹

2018

J. Biomed

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The recent technological and scientific development in the field of forensic biology is certainly stamping a huge impact in capturing and convicting the criminals without any doubt. This review enlists forthcoming emerging technologies in forensic biology which would become inevitable and routine tasks in laboratories for solving extremely challenging and critical cases. These include touch DNA profiling, forensic phenotyping, 3D facial reconstruction and morphometrics, microbial forensics, virtual autopsy, DNA methylation and next-generation sequencing methods which would prove boon sooner or later for the crime investigators in finding missing persons, nabbing murderers and sexual assaulters and solving an array of cold cases.

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Section: Conclusive Remarks and Future Prospectivementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Future of Forensic Biology

Rana¹

2018

J. Biomed

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“…DNA biochips enable the miniaturization, integration, and automation of tests, and can perform thousands of biological reactions in a few seconds [113][114][115]. Integrated and mobile rapid-DNA devices, which are designed for several purposes, can produce quality STR-profiles suitable for reference or as database samples [80,116].…”

Section: Extraction Of Compound And/or Challenging Samplesmentioning

confidence: 99%

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The importance of biological traces and evidences related to a criminal matter has been recognized for a long time. The examination of the expression of genetic polymorphism has been an integral part of the multidisciplinary field of medico-legal autopsy for over a century. Since the initial application of blood group antigens for personalization of a putative perpetrator in a murder case, the discipline of forensic genetics has evolved as a standard of forensic sciences. The real breakthrough, the application of molecular tools and processes for the in-vitro replication of genetic substances, has increasingly allowed the exploitation of advances of molecular genetics for both forensic and criminal investigations. Although there are certainly many more applications and scientific fields in the medico-legal arena, the relatively fast progress of genetics, which has accelerated recently with state-of-art technologies, can provide ever more relevant information in relation to a corpse or the cause and manner that resulted in the corpse for autopsy. This topic concerns the currently accepted forensic DNA technology, and the last section reviews commonly used markers for nuclear and mitochondrial DNA analysis as well as ongoing research. This review also focuses on the increasingly important non-human sources of DNA, and shortly covers the main aspects of animal forensic DNA examination.

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“…With characteristic of low cost, a rapid reaction rate and high efficiency, microfluidic chip integrated highly into micro-units implemented the functions in aspects of DNA analysis, drug screening, cell manipulation and immunology determination [1][2][3][4]. Traditionally, microfluidic chips were fabricated with several materials including silicon, glass and polymer.…”

Section: Introductionmentioning

confidence: 99%

Parameters identification of hot embossing machine based on the finite element method

Wang¹,

Sun²,

You³

et al. 2020

Preprint

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A new type of hot embossing machine was developed, which comprised of frame structure and pressing head. To ensure fitting accuracy of the pressing heads, static simulation of columns as vertical moving guide was conducted, simulation results demonstrated that the column of the frame structure possessed enough stiffness via adopting the material of Q235 and the size of 30 mm, and the largest radial deformation of the column reached to 2.5nm. Thermoelectric refrigeration reactor (TRR) as the improved heating mode was utilized and the power was determined. Because the TRRs did not bear the whole pressing load, different types of pressing head are developed, which achieved the separation of pressing load and TRR’s preload. Distributions of deformation on the feed direction were analysed for different types of pressing head. And simulation results demonstrated that the pressing head of "王" exhibited the best homogeneous deformation through comparing with other types of pressing head, which the maximum variation of deformation distribution approximately reached to 30nm. Additionally, force-thermal coupling simulation of pressing head plate as a core component was conducted to reduce thermal stress, and effects of distributions of deformation and temperature were discussed. For the given time, the head plate with the thickness of 18mm exhibited good uniformity of temperature and moderate heating rate, which the mentioned parameters reached 0.03°C/um 2 and 0.35°C/s, respectively. Generally, the applied thermal load decreases the deformation uniformity to a certain extent.

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The Optimization of Electrophoresis on a Glass Microfluidic Chip and its Application in Forensic Science

Cited by 16 publications

References 35 publications

The Future of Forensic Biology

The Future of Forensic Biology

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Parameters identification of hot embossing machine based on the finite element method

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