The Future of Forensic Biology

Rana, Ajay Kumar

doi:10.7150/jbm.22760

Cited by 6 publications

(2 citation statements)

References 59 publications

(65 reference statements)

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“…Semen can also be identified via Christmas tree staining or immunological based methods . However, the sensitivity and specificity of these tests can be affected by protein degradation and high dose hook effects .…”

Section: Introductionmentioning

confidence: 99%

Development of a body fluid identification multiplex via DNA methylation analysis

et al. 2019

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The goal of this study is to develop an epigenetic multiplex for body fluid identification based on tissue specific DNA methylation. A series of genetic loci capable of discerning the origin of DNA as coming from saliva, blood, vaginal epithelia, or semen were used for this application. The markers – BCAS4, CG06379435, VE_8, and ZC3H12D – were amplified together and then sequenced via pyrosequencing. Methylation values for cytosine guanine dinucleotide (CpG) sites at each locus were then measured across the four markers. In total, 124 samples were collected, and bisulfite modified to convert unmethylated DNA to uracil. This converted DNA was then amplified via multiplex PCR with reverse primers containing a biotin molecule. Biotinylated PCR products were then analyzed using pyrosequencing to generate a series of pyrograms containing 18 CpG sites. The percent methylation at each CpG site was determined, and then agglomerative hierarchical cluster analysis was used to create a model to indicate sample origin. Further analysis reduced the number of CpG sites required for optimal determination of body fluid type to five. This study demonstrates an efficient multiplexed body fluid identification process utilizing DNA methylation that can be easily implemented in forensic laboratories.

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Section: Introductionmentioning

confidence: 99%

Development of a body fluid identification multiplex via DNA methylation analysis

et al. 2019

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“…Kredibiliti DNA telah mengakibatkan DNA menjadi 'golden standard' untuk ahli forensik dan penting untuk penguatkuasaan undang-undang (Rana, 2018). Dalam konteks pembuktian, DNA yang diperolehi daripada barang-barang bukti mungkin berguna dalam meletakkan seseorang di tempat kejadian atau bersentuhan dengan objek yang berkaitan dengan perbuatan jenayah (Callaway, 2018).…”

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Analisis Awal Terhadap Isu-Isu Kebolehterimaan Asid Deoksiribonukleik DNA Sebagai Bahan Bukti di Mahkamah Syariah (Preliminary Analysis of Admissibility Issues of Deoxyribonucleic Acid DNA as Evidence in Syariah Court)

Ahmad

Razak

Saharudin

et al. 2019

UMRAN

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Deoxyribonucleic acid (DNA) benefits human life in various aspects. Islamic history has proven that the practice of proving a case through DNA has been carried out as early as the time of the Prophet Joseph (p.b.u.h.) and the time of the Prophet Muhammad (p.b.u.h.). A lot of efforts have been done to introduce evidence through DNA in the Syariah Court. However, there are some important issues that must be addressed by various parties in Malaysia before starting to apply the DNA evidence in the Syariah Court. This study was conducted to identify important issues that require attention in the realization of the use of DNA evidence in the Syariah Court. This qualitative study employed socio-legal and library-based research in collecting primary and secondary data. Then, the data have been analyzed by using content analysis method through inductive and deductive reasoning. This study found five issues that need to be addressed namely the knowledge and understanding of shariah officers in regard to the DNA, the need to establish Syariah Forensic Laboratory, the suspect's reluctance to provide his DNA sample for DNA analysis, DNA forensic expert qualification as an expert witness in Syariah Court, and the evidential value of DNA in Syariah Court. This study concludes that these issues require effective and practical solution for the effort to bring DNA evidence to be successfully carried out.

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