Psychromonas aquatilis sp. nov., isolated from seawater samples obtained in the Chilean Antarctica

Kämpfer, Peter; Irgang, Rute; Poblete‐Morales, Matías; Glaeser, Stefanie P.; Martín, Marcelo Cortez San; Avendaño‐Herrera, Rubén

doi:10.1099/ijsem.0.001801

Cited by 12 publications

(4 citation statements)

References 25 publications

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“…Growth capability of strain KUDC0405 T and the reference strains (M. bovistercoris NEAU-LLE T and M. pseudoresistence CC-5209 T ) at different temperatures (4, 10, 25, 30, 37, and 42°C) and different pH values (pH 3-12, in 1 pH unit intervals). The pH values were adjusted as described by Kämpfer et al [36] in sterilized TSA. The growth capability in the different NaCl concentrations (0-10% w/v, in 0.5% increments) was also evaluated in tryptic soy broth (TSB).…”

Section: Growth Capabilities and Motilitymentioning

confidence: 99%

Microbacterium elymi sp. nov., Isolated from the Rhizospheric Soil of Elymus tsukushiensis, a Plant Native to the Dokdo Islands, Republic of Korea

Hwang

Lee

Son

et al. 2023

J. Microbiol. Biotechnol.

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The genus Microbacterium was classified by Orla-Jensen S [1] based on the d-lactic acid-producing bacteria in milk. At that time, the genus Microbacterium comprised 157 species, including Microbacterium aerolatum V-73 T [2], M. agarici CC-SBCK-209 T Microbacterium can be isolated from various sources, such as seawater, desert soil, maize rhizosphere, cow dung, and microfiltered milk. Members of this genus are Gramstain-positive, rod-shaped, and have an optimum growth temperature of 20-30°C. Here, we report a taxonomic analysis of the novel bacterial strain, KUDC0405 T , isolated from the rhizospheric soil of Elymus tsukushiensis, a plant native to the Dokdo Islands (37°14′24.2′′ N, 131°52′12.2′′ E). During microbial diversity monitoring in April 2014, rhizospheric soil samples were collected from native plants of the Ulleungdo and Dokdo Islands (Ulleunggun, Gyeongsangbuk-do). E. tsukushiensis var. trasiens (Hack.) Osada is native to the Dokdo Islands and is the dominant plant species on these islands, and its distribution is expanding [9]. The Dokdo Islands are a group of volcanic islands located in the middle of the East Sea, east of the Republic of Korea (37°14′24.2′′N, 131°52′12.2′′E; Uljin-gun, Gyeongsangbuk-do). The island group consists of two large islands (Dong-do and Seo-do) and 89 small islets, and high concentrations of Ba, K, and Rb have been detected in soil [10]. Despite the difficult survival Microbacterium elymi KUDC0405 T was isolated from the rhizosphere of Elymus tsukushiensis from the Dokdo Islands. The KUDC0405 T strain was Gram-stain-positive, non-spore forming, non-motile, and facultatively anaerobic bacteria. Strain KUDC0405 T was a rod-shaped bacterium with size dimensions of 0.3-0.4 × 0.7-0.8 μm. Based on 16S rRNA gene sequences, KUDC0405 T was most closely related to Microbacterium bovistercoris NEAU-LLE T (97.8%) and Microbacterium pseudoresistens CC-5209 T (97.6%). The dDDH (digital DNA-DNA hybridization) values between KUDC0405 T and M. bovistercoris NEAU-LLE T and M. pseudoresistens CC-5209 T were below 17.3% and 17.5%, respectively. The ANI (average nucleotide identity) values among strains KUDC0405 T , M. bovistercoris NEAU-LLE T , and M. pseudoresistens CC-5209 T were 86.6% and 80.7%, respectively. The AAI (average amino acid identity) values were 64.66% and 64.97%, respectively, between KUDC0405 T and its closest related type strains. The genome contained 3,596 CDCs, three rRNAs, 46 tRNAs, and three non-coding RNAs (ncRNAs). The genomic DNA GC content was 70.4%. The polar lipids included diphosphatydilglycerol, glycolipid, phosphatydilglycerol, and unknown phospholipid, and the major fatty acids were anteiso-C 17:0 and iso-C 16:0 . Strain KUDC0405 T contained MK-12 as the major menaquinone. Based on genotypic, phylogenetic, and phenotypic properties, strain KUDC0405 T should be considered a novel species within the genus Microbacterium, for which we propose the name M. elymi sp. nov., and the type strain as KUDC0405 T (=KCTC 49411 T , =CGMCC1.18472 T ).

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Section: Growth Capabilities and Motilitymentioning

confidence: 99%

Microbacterium elymi sp. nov., Isolated from the Rhizospheric Soil of Elymus tsukushiensis, a Plant Native to the Dokdo Islands, Republic of Korea

Hwang

Lee

Son

et al. 2023

J. Microbiol. Biotechnol.

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“…Psychromonas aquatilis was isolated from untreated LO at the start of storage and from HPP-treated UL and LO at the start and end of storage. P. aquatilis was first isolated from an Antarctica water sample (Kämpfer et al, 2017). P. arctica, its closest relative, has been isolated from brown seaweeds U. pinnatifida (Lee et al, 2006) and Splachnidium rugosum (Albakosh et al, 2016).…”

Section: Main Bacterial Species Isolated From Seaweedsmentioning

confidence: 99%

Bacterial diversity in six species of fresh edible seaweeds submitted to high pressure processing and long-term refrigerated storage

Picón¹,

Olmo²,

Núñez³

2021

Food Microbiology

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“…The pH values were adjusted as described by Kampfer et al . [12]. The NaCl tolerance and growth in the absence of NaCl were tested at 0–20 % NaCl concentration (w/v; in 0.5 % increments) in TSB.…”

Section: Full-textmentioning

confidence: 99%

Sanguibacter suaedae sp. nov., isolated from the root of Suaeda aralocaspica in north-west PR China

Lei

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain, designated YZGR15T, was isolated from the root of an annual halophyte Suaeda aralocaspica, collected from the southern edge of the Gurbantunggut desert, north-west PR China. Cells of the isolate were Gram-stain-positive, facultatively anaerobic, irregular rods. Growth occurred at 4–42 °C (optimum, 30–37 °C), at pH 6.0–9.0 (optimum, pH 7.0–7.5) and in the presence of 0–9 % (w/v) NaCl (optimum, 2–5 %). Phylogenetic analysis using 16S rRNA gene sequences indicated that strain YZGR15T showed the highest sequence similarity to Sanguibacter keddieii (98.27 %), Sanguibacter antarcticus (98.20 %) and Sanguibacter inulinus (98.06 %). Results of genome analyses of strain YZGR15T indicated that the genome size was 3.16 Mb, with a genomic DNA G+C content of 71.9 mol%. Average nucleotide identity and digital DNA–DNA hybridization values between strain YZGR15Tand three type strains were in the range of 76.5–77.8 % and 20.0–22.2 %, respectively. Analysis of the cellular component of strain YZGR15T revealed that the primary fatty acids were anteiso-C15 : 0, C16 : 0, C14 : 0 and iso-C16 : 0 and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. The cell-wall characteristic amino acids were glutamic acid, alanine and an unknown amino acid. The whole-cell sugars for the strain were mannose, ribose, rhamnose, glucose and an unidentified sugar. The predominant respiratory quinone was MK-9(H4). Based on the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain YZGR15T represents a novel species of the genus Sanguibacter , for which the name Sanguibacter suaedae sp. nov. is proposed. The type strain is YZGR15T (=CGMCC 1.18691T=KCTC 49659T)

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Psychromonas aquatilis sp. nov., isolated from seawater samples obtained in the Chilean Antarctica

Cited by 12 publications

References 25 publications

Microbacterium elymi sp. nov., Isolated from the Rhizospheric Soil of Elymus tsukushiensis, a Plant Native to the Dokdo Islands, Republic of Korea

Microbacterium elymi sp. nov., Isolated from the Rhizospheric Soil of Elymus tsukushiensis, a Plant Native to the Dokdo Islands, Republic of Korea

Bacterial diversity in six species of fresh edible seaweeds submitted to high pressure processing and long-term refrigerated storage

Sanguibacter suaedae sp. nov., isolated from the root of Suaeda aralocaspica in north-west PR China

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