In vivo metabolomic interpretation of the anti‐obesity effects of hyacinth bean (<i>Dolichos lablab</i> L.) administration in high‐fat diet mice

Suh, Dong Ho; Lee, Hye Won; Jung, Eun Joo; Singh, Devendra; Kim, Seung-Hyung; Lee, Choong Hwan

doi:10.1002/mnfr.201600895

Cited by 22 publications

(18 citation statements)

References 31 publications

(41 reference statements)

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“…Discriminative variables were selected based on variable importance in the projection (VIP) value of the PLS-DA. The selected metabolites obtained from GC-TOF-MS and UHPLC-LTQ-Orbitrap-MS were tentatively identified based on various data comparing their retention time (min), mass spectrum (m/z), an MS n fragment pattern with those for standard compounds analyzed under identical conditions, and various, available databases including the Human Metabolome Database (HMDB, http://www.hmdb.ca/), the National Institute of Standards and Technology (NIST) database (Version 2.0, 2001, FairCom, Gaithersburg, MD, USA), Wiley 9, in house libraries, and published papers [51]. Significant differences were evaluated by analysis of variance (ANOVA) and Student' t-test coupled with the Pearson's correlation coefficient between metabolites and the corresponding phenotype using Predictive Analytics SoftWare (PASW) Statistics 18 software (SPSS Inc., Chicago, IL, USA).…”

Section: Discussionmentioning

confidence: 99%

Metabolite Profiling Revealed That a Gardening Activity Program Improves Cognitive Ability Correlated with BDNF Levels and Serotonin Metabolism in the Elderly

Park

Son

Lee

et al. 2020

IJERPH

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Metabolomics is useful for evaluating the fundamental mechanisms of improvements in the health functions of the elderly. Additionally, gardening intervention as a regular physical activity for the elderly maintained and improved physical, psychology, cognitive, and social health. This study was conducted to determine whether the cognitive ability of the elderly is affected by participating in a gardening activity program as a physical activity with a metabolomic potential biomarker. The gardening program was designed as a low to moderate intensity physical activity for the elderly. Serum metabolites resulting from gardening were subjected to metabolite profiling using gas chromatography time-of-flight mass spectrometry and ultra-high-performance liquid chromatography-linear trap quadruple-orbitrap-mass spectrometry followed by multivariate analyses. The partial least squares-discriminant analysis showed distinct clustering patterns among the control, non-gardening, and gardening groups. According to the pathway analysis, tryptophan metabolism including tryptophan, kynurenine, and serotonin showed significantly distinctive metabolites in the gardening group. Brain-derived neurotrophic factor levels (BDNF) in the gardening group were significantly increased after the gardening program. Correlation map analysis showed that the relative levels of tryptophan metabolites were positively correlated with BDNF. Our results show that tryptophan, kynurenine, and serotonin may be useful as metabolic biomarkers for improved cognitive ability by the gardening intervention.

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Section: Discussionmentioning

confidence: 99%

Metabolite Profiling Revealed That a Gardening Activity Program Improves Cognitive Ability Correlated with BDNF Levels and Serotonin Metabolism in the Elderly

Park

Son

Lee

et al. 2020

IJERPH

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“…It demonstrated that HEE could lower the risk of arteriosclerosis. Because lipids are valuable source and stores of metabolic energy, level changes of metabolites related to lipid in adipose have been regarded as the indicators of obesity‐related disorders (Green, Kwok & Durrington, ; Kim et al.,; Laaksonen et al., ; Suh et al., ).…”

Section: Resultsmentioning

confidence: 99%

Untargeted Metabolite Profiling of Adipose Tissue in Hyperlipidemia Rats Exposed to Hawthorn Ethanol Extracts

Zhang

Liu

et al. 2019

Journal of Food Science

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The study aimed to explore the metabolic changes of adipose tissue of hyperlipidemia rats with hawthorn ethanol extracts (HEE) consumption by a high‐throughput metabolomics approach. HEE were mainly composed of chlorogenic acid, hyperoside, isoquercitrin, rutin, vitexin, quercetin, and apigenin by HPLC analysis. HEE administration significantly lowered levels of the total cholesterols, triglyceride and low‐density lipoprotein cholesterol as compared to the high‐fat diet model. Gas chromatography‐mass spectrometry was used to identify adipose metabolite profiles. Numerous endogenous molecules were altered by high‐fat diet and restored following intervention of HEE. Metabolites elevated in adipose, including l‐threonine, aspartic acid, glutamine, mannose, inositol and oleic acid, were detected after HEE consumption. Fifteen metabolites were identified as potential biomarkers of hyperlipidemia. Pathway analysis showed that most of the discriminant metabolites were included in fatty acid biosynthesis, galactose metabolism, biosynthesis of unsaturated fatty acids, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, glycerolipid metabolism and steroid biosynthesis. These metabolites and metabolic networks we found offer new insights into exploring the molecular mechanisms of lipid‐lowering of hawthorn ethanol extracts on adipose tissue of rats. Practical Application There was a very high proportion of hyperlipidemia in China. Hawthorn is attracting increasing attention owing to their health benefits, low toxicity, effectiveness and might be suitable for long‐term use.

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“…After centrifugation (12,578 g , 4°C, 10 min), the supernatant was filtered using a 0.2-μm PTFE filter, and dried using a speed vacuum concentrator. The dried extracts were re-dissolved with 80% methanol (10mg/ml) for UPLC-Q-TOF-MS analysis under previously described analytical conditions [ 16 ]. For GC-TOF-MS analysis, dried samples were oximated with 50 μL of methoxyamine hydrochloride (20 mg/mL in pyridine) for 90 min at 30°C, and silylated with 50 μL of MSTFA for 30 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

The green tea modulates large intestinal microbiome and exo/endogenous metabolome altered through chronic UVB-exposure

et al. 2017

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The attenuating effects of green tea supplements (GTS) against the ultraviolet (UV) radiation induced skin damages are distinguished. However, the concomitant effects of GTS on the large intestinal microbiomes and associated metabolomes are largely unclear. Herein, we performed an integrated microbiome-metabolome analysis to uncover the esoteric links between gut microbiome and exo/endogenous metabolome maneuvered in the large intestine of UVB-exposed mice subjected to dietary GTS. In UVB-exposed mice groups (UVB), class Bacilli and order Bifidobacteriales were observed as discriminant taxa with decreased lysophospholipid levels compared to the unexposed mice groups subjected to normal diet (NOR). Conversely, in GTS fed UVB-exposed mice (U+GTS), the gut-microbiome diversity was greatly enhanced with enrichment in the classes, Clostridia and Erysipelotrichia, as well as genera, Allobaculum and Lachnoclostridium. Additionally, the gut endogenous metabolomes changed with an increase in amino acids, fatty acids, lipids, and bile acids contents coupled with a decrease in nucleobases and carbohydrate levels. The altered metabolomes exhibited high correlations with GTS enriched intestinal microflora. Intriguingly, the various conjugates of green tea catechins viz., sulfated, glucuronided, and methylated ones including their exogenous derivatives were detected from large intestinal contents and liver samples. Hence, we conjecture that the metabolic conversions for the molecular components in GTS strongly influenced the gut micro-environment in UVB-exposed mice groups, ergo modulate their gut-microbiome as well as exo/endogenous metabolomes.

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In vivo metabolomic interpretation of the anti‐obesity effects of hyacinth bean (Dolichos lablab L.) administration in high‐fat diet mice

Abstract: A lower dose of hyacinth bean resulted in similar anti-obesity effects as milk thistle, as confirmed by both clinical and metabolomics analyses. Equivocally, we conjecture that hyacinth bean could be used as a potent anti-obesity herbal functional food.

Cited by 22 publications

References 31 publications

Metabolite Profiling Revealed That a Gardening Activity Program Improves Cognitive Ability Correlated with BDNF Levels and Serotonin Metabolism in the Elderly

Metabolite Profiling Revealed That a Gardening Activity Program Improves Cognitive Ability Correlated with BDNF Levels and Serotonin Metabolism in the Elderly

Untargeted Metabolite Profiling of Adipose Tissue in Hyperlipidemia Rats Exposed to Hawthorn Ethanol Extracts

The green tea modulates large intestinal microbiome and exo/endogenous metabolome altered through chronic UVB-exposure

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