Arabidopsis phosphoinositide‐specific phospholipase C 4 negatively regulates seedling salt tolerance

Abstract

Previous physiological and pharmacological studies have suggested that the activity of phosphoinositide-specific phospholipase C (PI-PLC) plays an important role in regulating plant salt stress responses by altering the intracellular Ca concentration. However, the individual members of plant PLCs involved in this process need to be identified. Here, the function of AtPLC4 in the salt stress response of Arabidopsis seedlings was analysed. plc4 mutant seedlings showed hyposensitivity to salt stress compared with Col-0 wild-type seedlings, and the salt hyposensitive phenotype could be complemented by the expression of native promoter-controlled AtPLC4. Transgenic seedlings with AtPLC4 overexpression (AtPLC4 OE) exhibited a salt-hypersensitive phenotype, while transgenic seedlings with its inactive mutant expression (AtPLC4m OE) did not exhibit this phenotype. Using aequorin as a Ca indicator in plc4 mutant and AtPLC4 OE seedlings, AtPLC4 was shown to positively regulate the salt-induced Ca increase. The salt-hypersensitive phenotype of AtPLC4 OE seedlings was partially rescued by EGTA. An analysis of salt-responsive genes revealed that the transcription of RD29B, MYB15 and ZAT10 was inversely regulated in plc4 mutant and AtPLC4 OE seedlings. Our findings suggest that AtPLC4 negatively regulates the salt tolerance of Arabidopsis seedlings, and Ca may be involved in regulating this process.

Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana

“…In rice, PLC1-mediated Ca 2+ signaling is essential for controlling the accumulation of Na + that leads to salt tolerance ( Li et al , 2017 ). In contrast, AtPLC4 negatively regulates the salt tolerance of Arabidopsis seedlings through Ca 2+ regulatory processes ( Xia et al , 2017 ). PLC2 is involved in stress responses related to the endoplasmic reticulum in Arabidopsis ( Kanehara et al , 2015 ).…”

De novo transcriptome sequencing of two cultivated jute species under salinity stress

“…In addition, NHXs could compartmentalize Na+ in vacuoles to prevent toxic effects in the cytosol. In the study, numerous DEGs encoding HKT (1, up-regulated), CIPK (27, 17 up-regulated,), SOS1 (2, up-regulated) and NHX (3, up and down-regulated) were found[ 29 ]. The DEGs might be play crucial role in Na+ homeostasis.…”

Single cell‐type analysis of cellular lipid remodelling in response to salinity in the epidermal bladder cells of the model halophyte Mesembryanthemum crystallinum

“…Specifically, phosphoinositides [PIPs; phosphorylated phosphatidylinositol (PI) species] and phosphatidic acid (PA) have been shown to directly affect membrane curvature and charge as well as play a role in recruiting target proteins to particular membranes influencing their localization and/or activity (Bargmann & Munnik, ; Munnik & Vermeer, ; Testerink & Munnik, ). A universal role of these signalling lipids in plant osmotic stress response has been proposed, having been proven to be responsive to salt treatment (Bargmann et al, ; Darwish, Testerink, Khalil, El‐Shihy, & Munnik, ; Meijer, van Himbergen, Musgrave, & Munnik, ; Meringer et al, ; Xia et al, ; Zonia & Munnik, ). Nevertheless, the majority of these studies have been performed in salt‐sensitive photosynthetic organisms.…”