Heliangin inhibited lipopolysaccharide-induced inflammation through signaling NF-κB pathway on LPS-induced RAW 264.7 cells

Lu, Xianghua; Li, Min; Wei, Jionglin; Gou, Haixin; Bao, Zhijun; Wang, Jiao-feng; Wang, Zheng; Huang, Yizhi; An, Bing-Chen

doi:10.1016/j.biopha.2017.01.041

Cited by 26 publications

(19 citation statements)

References 37 publications

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“…Inflammation involves many complex interactions between cellular and inflammatory mediators. During the inflammatory process, significant amounts of NO and prostaglandin E2 are produced via the expression of iNOS and COX-2 (Lu et al 2017). The excessive production of proinflammatory mediators by macrophages can induce variety of inflammation-related disorders; thus, agents that suppress the production of these factors may have the potential to protect against inflammatory diseases (Jung et al 2007;Yun et al 2014;Song et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Berköz

2019

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Diosmin is an unsaturated flavonoid glycoside, presents in citrus fruits. The aim of this study is to investigate the molecular mechanism of diosmin with respect to the NF-κB and MAPKs signaling pathways. Firstly, 10, 20, 30, 40 and 50 µM diosmin were treated to lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The anti-inflammatory effects of diosmin was displayed via measuring prostaglandin E 2 (PGE 2), nitric oxide (NO), interleukines (IL-6, IL-12), tumor necrosis factor α (TNF-α) production, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), IL-6, IL-12, TNF-α mRNA levels, and phosphorylation levels of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκB-α) and mitogen-activated protein kinases (MAPKs); JNK, ERK, and p38 in LPS induced RAW264.7 macrophages. Our study showed that especially high concentrations of diosmin decreased NO, PGE 2 , IL-6, IL-12, TNF-α production and mRNA levels of these mediators (p < 0.05). The expression of phosphorylated-JNK was significantly suppressed by diosmin at 40 and 50 µM concentrations. Furthermore, diosmin significantly inhibited the expression of phosphorylated-ERK, p38, and p-IκB-α in a dose-dependent manner. Our results suggest that diosmin is a potent anti-inflammatory agent and has potential for development into a therapeutic agent for inflammation-associated disorders.

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Section: Discussionmentioning

confidence: 99%

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Berköz

2019

gpb

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“…Inflammation involves many complex interactions between cellular and inflammatory mediators. During the inflammatory process, significant amounts of NO and prostaglandin E2 are produced via the expression of iNOS and COX-2 [ 12 ]. The excessive production of proinflammatory mediators by macrophages can induce variety of inflammation-related disorders [ 3 , 5 ]; thus, agents that suppress the production of these factors may have the potential to protect against inflammatory diseases.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Anti‐Inflammatory Effects of Flavonoid‐Rich Common and Tartary Buckwheat Sprout Extracts in Lipopolysaccharide‐Stimulated RAW 264.7 and Peritoneal Macrophages

Nam

Lim

Lee

et al. 2017

Oxidative Medicine and Cellular Longevity

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Buckwheat sprouts have been widely consumed all around world due to their great abundance of bioactive compounds. In this study, the anti-inflammatory effects of flavonoid-rich common buckwheat sprout (CBS) and tartary buckwheat sprout (TBS) extracts were evaluated in lipopolysaccharide- (LPS-) stimulated RAW 264.7 murine macrophages and primary peritoneal macrophages from male BALB/c mice. Based on the reversed-phase HPLC analysis, the major flavonoids in CBS were determined to be C-glycosylflavones (orientin, isoorientin, vitexin, and isovitexin), quercetin-3-O-robinobioside, and rutin, whereas TBS contained only high amounts of rutin. The TBS extract exhibited higher inhibitory activity as assessed by the production of proinflammatory mediators such as nitric oxide and cytokines including tumor necrosis factor-α, interleukin- (IL-) 6, and IL-12 in LPS-stimulated RAW 264.7 macrophages than CBS extract. In addition, TBS extract suppressed nuclear factor-kappa B activation by preventing inhibitor kappa B-alpha degradation and mitogen-activated protein kinase phosphorylation in LPS-stimulated RAW 264.7 macrophages. Moreover, the TBS extract markedly reduced LPS-induced cytokine production in peritoneal macrophages. Taken together, these findings suggest that TBS extract can be a potential source of anti-inflammatory agents that may influence macrophage-mediated inflammatory disorders.

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“…When LPS acts on biological cells, its effects can be observed through TLR4 in the cell membrane [28]. The TLR family is related to the expression of in ammatory proteins, cytokines and plays an important role in natural immunity and in ammation [29]. An LPS-induced RAW264.7 cell in ammation model is usually used to demonstrate the physiological activities of plant extracts or active substances.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of Camellia japonica on cell inflammation and acute rat reflux esophagitis

Nam

Choo

2020

Preprint

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Background: Oxidative stress and inflammation are the causes of many diseases. Reflux esophagitis (RE) is a common gastroesophageal reflux disease with a continuous upward trend in incidence worldwide. Camellia japonica (C. japonica) has long been used as a traditional herbal hemostatic medicine in China and Korea. The purpose of this study was to explore the anti-oxidant and anti-inflammatory activities of C. japonica and its protective effect against RE.Materials and methods: Buds from C. japonica plants were collected in a mountainous area of Jeju, South Korea. Dried C. japonica buds were extracted with 75% ethanol. DPPH and ABTS radical scavenging assays were performed. The ROS production and anti-inflammatory effects of C. japonica bud ethanol extract (CJE) on LPS-induced RAW264.7 cell inflammation were evaluated. The protective effects of CJE on RE were investigated in a RE rat model. Results: CJE eliminated over 50% of DPPH and ABTS radicals at concentrations of 100 and 200 μg/mL, respectively. CJE alleviated changes in cell morphology, reduced production of ROS, NO and IL-1β and down-regulated expression levels of iNOS, TNF-α, phosphorylated NF-κB, IκBα, and JNK/p38/MAPK. CJE reduced the esophageal tissue damage ratio (40.3%) and attenuation of histological changes. In addition, CJE down-regulated expression levels of TNF-α, IL-1β, and COX-2 and phosphorylation levels of NF-κB and IκBα in esophageal tissue. Conclusions: CJE possesses good anti-oxidation and anti-inflammatory activity, and can reduce RE damage in rats caused by gastric acid reflux. Therefore, CJE is a natural material that may be a candidate phytomedicine for the treatment of RE.

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Heliangin inhibited lipopolysaccharide-induced inflammation through signaling NF-κB pathway on LPS-induced RAW 264.7 cells

Cited by 26 publications

References 37 publications

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Comparison of Anti‐Inflammatory Effects of Flavonoid‐Rich Common and Tartary Buckwheat Sprout Extracts in Lipopolysaccharide‐Stimulated RAW 264.7 and Peritoneal Macrophages

Inhibitory effects of Camellia japonica on cell inflammation and acute rat reflux esophagitis

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Heliangin inhibited lipopolysaccharide-induced inflammation through signaling NF-κB pathway on LPS-induced RAW 264.7 cells

Cited by 26 publications

References 37 publications

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Diosmin suppresses the proinflammatory mediators in lipopolysaccharide-induced RAW264.7 macrophages via NF-κB and MAPKs signal pathways

Comparison of Anti‐Inflammatory Effects of Flavonoid‐Rich Common and Tartary Buckwheat Sprout Extracts in Lipopolysaccharide‐Stimulated RAW 264.7 and Peritoneal Macrophages

Inhibitory effects of Camellia japonica&nbsp;on cell inflammation and acute rat reflux esophagitis

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Inhibitory effects of Camellia japonica on cell inflammation and acute rat reflux esophagitis