Inhibition of invasion by N -trans -feruloyloctopamine via AKT, p38MAPK and EMT related signals in hepatocellular carcinoma cells

Bai, Zhongtian; Wu, Zhengrong; Xi, Lili; Li, Xun; Chen, Peng; Wang, Fuqiang; Meng, Wenbo; Zhou, Wence; Wu, Xinan; Yao, Xiaojun; Zhang, Min

doi:10.1016/j.bmcl.2016.12.073

Cited by 25 publications

(21 citation statements)

References 21 publications

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“…However, little is known about the regulatory role of Trop2 in TNBC. E-cadherin is a type of adhesion molecule that mainly exists in human and animal epithelium, and its main function is to maintain normal epithelial cell morphology and structural integrity (13)(14)(15). E-cadherin can regulate the close adhesion of epithelial cells and its downregulation is a key factor in WEI ZHAO 1* , XINGWANG KUAI 2* , XUEYI ZHOU 3 , LIZHOU JIA 2 , JINSONG WANG 1 , XIAOBING YANG 1 , ZHIDAN TIAN 1 , XIAOLEI WANG 1 , QIAN LV 1 , BIN WANG 1 , YOUCAI ZHAO 1 and WENBIN HUANG 1 epithelial neoplasm development (16).…”

Section: Introductionmentioning

confidence: 99%

Trop2 is a potential biomarker for the promotion of EMT in human breast cancer

et al. 2018

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Trop2 is considered to have an important function in tumor metastasis and the promotion of epithelial‑mesenchymal transition (EMT). E‑cadherin is a crucial factor in intercellular adhesion and EMT transformation. In the present study, we detected the expression of Trop2 and E‑cadherin in breast cancer (BC) to better define their prognostic value. The mRNA expression levels of these two genes in 20 cases of fresh BC tissues were detected by quantitative real‑time polymerase chain reaction (qRT‑PCR). We also detected the expression levels of these two genes by immunohistochemistry (IHC) in 312 BC tissues, and the correlations between the expression of these two genes and the clinicopathological characteristics in BC patients were analyzed. The mRNA and protein expression levels of the two genes in BC cell lines were studied by qRT‑PCR and western blotting. The results indicated that Trop2+/E‑cadherin‑ was expressed in BC tissues more than that in the matched adjacent tissues. The protein expression results obtained via IHC were similar to the mRNA expression results. Trop2+/E‑cadherin‑ that was expressed in BC was associated with lymph node status, metastasis, tumor‑node‑metastasis (TNM) stage, and ER‑/PR‑/HER2‑ expression. BC patients that expressed Trop2+/E‑cadherin‑ had poor overall survival rates. The results of Trop2 and E‑cadherin expression levels obtained in the BC cell lines were the same as those obtained in the BC tissues. Overall, Trop2 has a potential role in the promotion of EMT in BC and it could be considered as a therapeutic target in the future.

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Section: Introductionmentioning

confidence: 99%

Trop2 is a potential biomarker for the promotion of EMT in human breast cancer

et al. 2018

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“…In the meantime, the reduction of p38MAPK, a member of the MAPK family [16], can elicit the tumor-suppressing effect in cervical cancer [17]. Moreover, it has been also observed that cell invasion might be inhibited through EMT-related signals (E-cadherin) directly or p38 MAPK signaling pathways indirectly [18]. Nevertheless, whether ZEB1-AS1 can regulate p38 MAPK signaling pathway to affect the formation of EMT in cervical cancer is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Long Non-Coding RNA ZEB1-Antisense 1 Affects Cell Migration and Invasion of Cervical Cancer by Regulating Epithelial-Mesenchymal Transition via the p38MAPK Signaling Pathway

Gan¹,

Chen²,

Liu³

et al. 2018

Gynecol Obstet Invest

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Aim: To investigate whether long non-coding RNA (lncRNA) ZEB1 antisense 1 (ZEB1-AS1) affects cell migration and invasion of cervical cancer by regulating epithelial-mesenchymal transition (EMT) via the p38MAPK pathway. Methods: Human cervical cancer cell line Hela was classified into Control, NC siRNA, ZEB1-AS1 siRNA, SB203580 (p38MAPK pathway inhibitor) and ZEB1-AS1 siRNA + Anisomycin (p38MAPK pathway activator) groups. Quantitative real-time polymerase chain reaction was performed for ZEB1-AS1 expression, Western blotting to measure p38MAPK signaling pathway-/EMT-related proteins, and Wound-healing and Transwell assays to evaluate cell migration and invasion respectively. Results: ZEB1-AS1 was upregulated in cancer tissues and related to major clinicopathological features of cervical cancer. Besides, patients with lower-ZEB1-AS1-expression had a higher 5-year survival rate than those patients with higher-ZEB1-AS1-expression. High ZEB1-AS1 expression and advanced Federation of Gynecology and Obstetrics stage were independent risk factors for patients’ prognosis. Both ZEB1-AS1 siRNA and SB203580 effectively reduced p-p38 expression and the migration and invasion of Hela cells, with elevation of E-cadherin and reduction of Vimentin and N-cadherin. However, inhibitory effects of ZEB1-AS1 siRNA on EMT as well as cell migration and invasion of the Hela cell were reversed by Anisomycin. Conclusion: Inhibition of ZEB1-AS1 can block the p38MAPK signaling pathway, ultimately restricting the EMT and suppressing cell migration and invasion of cervical cancer cells.

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“…22 P38MAPK and extracellular regulated protein kinases (ERKs) are closely related to HCC invasion, migration, and apoptosis. [23][24][25] Our study found that DFS was significantly prolonged in the P38MAPK high expression group and that P38MAPK may play a role as a tumor suppressor gene (Figure 2E, P=0.0033).…”

Section: Discussionmentioning

confidence: 67%

<p>High Inflammatory Factor Grading Predicts Poor Disease-Free Survival in AJCC Stage I-II Hepatocellular Carcinoma Patients After R0 Resection</p>

Zhang

Chua

et al. 2019

CMAR

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Purpose: In this study, we established the inflammatory factor grade system (IFGs) based on the hepatocellular carcinoma (HCC) microenvironment to investigate the role of inflammatory factor grade (IFG) in predicting the prognosis of patients with American Joint Committee on Cancer (AJCC) stage I-II. Patients and methods: We enrolled 87 HCC patients with AJCC stage I-II who underwent R0 resection between 2000 and 2012 and had paraffin-embedded specimens. Immunohistochemistry (IHC) was performed to investigate the expression of 12 inflammatory factors and then to establish the IFGs (grade A or B) based on the IHC data. Subsequently, Kaplan-Meier and Cox univariate/ multivariate survival analyses were performed to examine the potential prognostic significance. Results: Higher IFG (IFG-B) is significantly associated with greater tumor size (P=0.037), and IFG-B predicts a worse disease-free survival (DFS, P<0.001). Moreover, a platelet count (PLT) ≤100×10 9 /L, tumor size ≥5 cm, poor tumor differentiation, and IFG-B are independent risk factors for DFS. Conclusion: Overall, by establishing a grading system for the level of inflammatory factors in the HCC microenvironment, IFG-B can effectively predict poor DFS in AJCC stage I-II HCC patients after R0 resection.

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Inhibition of invasion by N -trans -feruloyloctopamine via AKT, p38MAPK and EMT related signals in hepatocellular carcinoma cells

Cited by 25 publications

References 21 publications

Trop2 is a potential biomarker for the promotion of EMT in human breast cancer

Trop2 is a potential biomarker for the promotion of EMT in human breast cancer

Long Non-Coding RNA ZEB1-Antisense 1 Affects Cell Migration and Invasion of Cervical Cancer by Regulating Epithelial-Mesenchymal Transition via the p38MAPK Signaling Pathway

<p>High Inflammatory Factor Grading Predicts Poor Disease-Free Survival in AJCC Stage I-II Hepatocellular Carcinoma Patients After R0 Resection</p>

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