We describe an optical transduction mechanism to measure the flexural mode vibrations of vertically aligned nanowires on a flat substrate with high sensitivity, linearity, and ease of implementation. We demonstrate that the light reflected from the substrate when a laser beam strikes it parallel to the nanowires is modulated proportionally to their vibration, so that measuring such modulation provides a highly efficient resonance readout. This mechanism is applicable to single nanowires or arrays without specific requirements regarding their geometry or array pattern, and no fabrication process besides the nanowire generation is required. We show how to optimize the performance of this mechanism by characterizing the split flexural modes of vertical silicon nanowires in their full dynamic range and up to the fifth mode order. The presented transduction approach is relevant for any application of nanowire resonators, particularly for integrating nanomechanical sensing in functional substrates based on vertical nanowires for biological applications. N anomechanical sensing with resonant devices based on semiconductor nanowires (SCNWs) is currently consolidated as one of the most fruitful research lines involving this kind of nanostructure. SCNWs are highly useful as resonators for high-performance applications because of their unique structural and dynamic properties. The former imply an exceptional combination of very low mass, single-crystal quality, and controllable dimensions and geometry. 1 Regarding dynamic properties, the characteristic high aspect ratio of SCNWs results in flexural modes which are extremely sensitive to external perturbations of a different nature. 2 On one hand, this facilitates detecting their thermomechanical vibrations, as the effect of temperature alone results in relatively large vibration amplitudes; on the other hand, it also simplifies external driving schemes, as external forces of a different nature can be used to drive flexural vibrations even beyond the fundamental mode. These properties provide SCNW resonators with singular sensing capacities which are otherwise impossible to achieve with other structures. Some of the most significant ones are based on the splitting of each flexural mode in a doublet of nearly degenerate orthogonal oscillations, which occurs for both single-clamp 3,4 and double-clamp nanowires. 5 This effect has indeed already been explored for innovative applications such as simultaneous mass and elasticity sensing of adsorbates, 4,6 vectorial force sensing/imaging, 7−9 and the exploration of complex dynamics 10−13 or optomechanical back-action effects. 14,15 Exploiting the unique properties of SCNW resonators for developing an equally high performance and practical functionalities requires an efficient conversion of the nanowire vibrations into readable signals with high sensitivity, linearity, and minimum constraints regarding the nanowires' arrangement. Different transduction schemes have been demonstrated so far to that end, but arguably all of them have ...