Increasing NO level regulates apoptosis and inflammation in macrophages after 2-chloroethyl ethyl sulphide challenge

Sagar, Satish; Parida, Soumya; Sabnam, Silpa; Rizwan, Huma; Pal, Sweta; Swain, Mitali Madhusmita; Pal, Arttatrana

doi:10.1016/j.biocel.2016.12.004

Cited by 16 publications

(21 citation statements)

References 55 publications

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“…23 For the determination of protein carbonylation (PC), 80 µg (450 µL) protein samples were mixed with 450 µL of PBS, 100 µL of 10% streptomycin sulfate, and centrifuged at 1,000× g for 10 min. Then, 400 µL of the supernatant was mixed with an equal volume of 10 mM 2,4-dinitrophenylhydrazine and kept for 30 min in the dark.…”

Section: Estimation Of Biomolecules Damagementioning

confidence: 99%

“…Ice-cold trichloroacetic acid (20%) was added to the reaction mixture and centrifuged at 1,000× g. The pellet was dissolved in 6 M guanidine hydrochloride and absorbance was recorded. 23 Comet assay was carried out as described previously. 23 The DNA ladder assay was carried out as described previously.…”

Section: Estimation Of Biomolecules Damagementioning

confidence: 99%

“…23 Comet assay was carried out as described previously. 23 The DNA ladder assay was carried out as described previously. 11 …”

Section: Estimation Of Biomolecules Damagementioning

confidence: 99%

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Gold nanoparticles reduce high glucose-induced oxidative-nitrosative stress regulated inflammation and apoptosis via tuberin-mTOR/NF-κB pathways in macrophages

Rizwan

Mohanta

et al. 2017

IJN

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Hyperglycemia is a risk factor for cardiovascular mortality and morbidity, and directly responsible for exacerbating macrophage activation and atherosclerosis. We showed that gold nanoparticles (AuNPs) reduce the high glucose (HG)-induced atherosclerosis-related complications in macrophages via oxidative-nitrosative stress-regulated inflammation and apoptosis. The effects of AuNPs on oxidative-nitrosative stress markers such as cellular antioxidants were attenuated by HG exposure, leading to reduction in the accumulation of reactive oxygen/nitrogen species in cellular compartments. Further, these abnormalities of antioxidants level and reactive oxygen/nitrogen species accumulations initiate cellular stress, resulting in the activation of nuclear factor κB (NF-κB) via ERK1/2mitogen-activated protein kinase (MAPK)/Akt/tuberin-mammalian target of rapamycin (mTOR) pathways. The activated NF-κB stimulates inflammatory mediators, which subsequently subdue biomolecules damage, leading to aggravation of the inflammatory infiltration and immune responses. Treatment of AuNPs inhibits the intracellular redox-sensitive signaling pathways, inflammation, and apoptosis in macrophages. Together, our results indicate that AuNPs may modulate HG-induced oxidative-nitrosative stress. These effects may be sealed tight due to the fact that AuNPs treatment reduces the activation of NF-κB by ERK1/2MAPK/Akt/tuberin-mTOR pathways-mediated inflammatory genes expression and cellular stress responses, which may be beneficial for minimizing the atherosclerosis.

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Section: Estimation Of Biomolecules Damagementioning

confidence: 99%

Section: Estimation Of Biomolecules Damagementioning

confidence: 99%

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Gold nanoparticles reduce high glucose-induced oxidative-nitrosative stress regulated inflammation and apoptosis via tuberin-mTOR/NF-κB pathways in macrophages

Rizwan

Mohanta

et al. 2017

IJN

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“…Furthermore, the intracellular nitric oxide (NO) release is also involved in the signal transduction of inflammatory responses (Sagar et al, 2017). It is important to inhibit the over-production of NO in response to inflammatory stimuli, which can induce proinflammatory responses in inflammatory disorders (Lively & Schlichter, 2018).…”

Section: Effect Of Gypenosides On the Secretion Of Proinflammatory mentioning

confidence: 99%

Chemical composition of tetraploid Gynostemma pentaphyllum gypenosides and their suppression on inflammatory response by NF‐κB/MAPKs/AP‐1 signaling pathways

Wang

Gao

et al. 2020

Food Science & Nutrition

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The chemical composition and anti‐inflammatory activity of gypenosides isolated from tetraploid Gynostemma pentaphyllum (GP) leaves were investigated. The gypenosides accounted for 7.43 mg/g of the tested GP sample, which were composed of four major saponins including isomers of gypenoside 1 and 2 (C47H76O18), 3 (C47H76O17), and 4 (C46H74O17). Pretreatment of gypenosides reduced mRNA expressions of the proinflammatory mediators in LPS‐stimulated RAW264.7 macrophage cells, such as IL‐6, IL‐1β, COX‐2, and TNF‐α in a dose‐dependent manner. The secreted protein levels of IL‐6 and TNF‐α, and NO production were also decreased by gypenosides within the concentration range of 50–200 μg/ml. Moreover, the mechanism studies demonstrated that gypenosides (200 μg/ml) treatment significantly inhibited the nuclear translocation of nuclear factor‐κB and activator protein 1 (c‐Fos and c‐Jun) through down‐regulating the phosphorylation of their upstream IκB kinase and mitogen‐activated protein kinases (MAPKs), especially that of c‐Jun N‐terminal kinase and extracellular regulated protein kinase(JNK and ERK), but not that of the p38 MAPK. These results suggested that the gypenosides might have potential anti‐inflammatory effect and use for improving human health.

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“…Finally, the fifth group contains N ω -nitro-L-arginine methyl ester hydrochloride (L-NAME), a nitric oxide synthase inhibitor. Generation of nitric oxide activates apoptosis and inflammatory signals after CEES challenge (Sagar et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Screening of the chemoprotective effect of 13 compounds and their mixtures with sodium 2-mercaptoethanesulfonate against 2-chloroethyl ethyl sulfide

Jost¹,

Pejchal²,

Kučera³

et al. 2019

JAB

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2-chloroethyl ethyl sulfide (CEES) is a vesicant agent, commonly referred to half mustard due to its ability to form monofunctional adducts with DNA. In this study, we evaluated the chemoprotective potential of 13 compounds and their mixtures with sodium 2-mercaptoethanesulfonate (MESNA) against CEES-induced geno-and cytotoxicity in human lung cell line A-549. MESNA, L-glutathione (GSH), thiourea, sodium thiosulfate, hexamethylenetetramine, 4-acetamidophenol, asoxime dichloride (HI-6), N-acetyl-L-cysteine (NAC), sodium pyruvate, myo-inositol, 3-aminobenzamide (3-AB), nicotinamide, and N ω-nitro-L-arginine methyl ester hydrochloride and combinations of these compounds with MESNA were applied 30 min before CEES. DNA alkylation was measured using modified comet assay 1 and 24 h after the exposure. Cell viability was determined using MTT assay at 24 and 72 h. The mono-therapeutical approach identified MESNA and GSH to provide significant chemoprotection. NAC and 3-AB supported DNA damage repair, while cell viability remained unaffected. Mixtures of GSH or NAC with MESNA showed protective synergism against DNA damage. Other compounds or their combinations with MESNA failed due to the potentiation of CEES-induced cytotoxicity. The chemoprotection against CEES remains limited; however, the combination of substances can provide protective synergy and may represent a promising strategy in the treatment of accidental exposure to monoalkylating agents.

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Increasing NO level regulates apoptosis and inflammation in macrophages after 2-chloroethyl ethyl sulphide challenge

Cited by 16 publications

References 55 publications

Gold nanoparticles reduce high glucose-induced oxidative-nitrosative stress regulated inflammation and apoptosis via tuberin-mTOR/NF-κB pathways in macrophages

Gold nanoparticles reduce high glucose-induced oxidative-nitrosative stress regulated inflammation and apoptosis via tuberin-mTOR/NF-κB pathways in macrophages

Chemical composition of tetraploid Gynostemma pentaphyllum gypenosides and their suppression on inflammatory response by NF‐κB/MAPKs/AP‐1 signaling pathways

Screening of the chemoprotective effect of 13 compounds and their mixtures with sodium 2-mercaptoethanesulfonate against 2-chloroethyl ethyl sulfide

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Increasing NO level regulates apoptosis and inflammation in macrophages after 2-chloroethyl ethyl sulphide challenge

Cited by 16 publications

References 55 publications

Gold nanoparticles reduce high glucose-induced oxidative-nitrosative stress regulated inflammation and apoptosis via tuberin-mTOR/NF-&kappa;B pathways in macrophages

Gold nanoparticles reduce high glucose-induced oxidative-nitrosative stress regulated inflammation and apoptosis via tuberin-mTOR/NF-&kappa;B pathways in macrophages

Chemical composition of tetraploid Gynostemma pentaphyllum gypenosides and their suppression on inflammatory response by NF‐κB/MAPKs/AP‐1 signaling pathways

Screening of the chemoprotective effect of 13 compounds and their mixtures with sodium 2-mercaptoethanesulfonate against 2-chloroethyl ethyl sulfide

Contact Info

Product

Resources

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Gold nanoparticles reduce high glucose-induced oxidative-nitrosative stress regulated inflammation and apoptosis via tuberin-mTOR/NF-κB pathways in macrophages

Gold nanoparticles reduce high glucose-induced oxidative-nitrosative stress regulated inflammation and apoptosis via tuberin-mTOR/NF-κB pathways in macrophages