Gastroprotective activity of the resin from Virola oleifera

Abstract

Context: The resin from the trunk wood of Virola oleifera (Schott) A. C. Smith (Myristicaceae) is used in folk medicine to hasten wound repair and to treat pain and inflammatory conditions, and our previous report indicated the anti-oxidative properties in other oxidative stress model.Objective: To investigate the protective effects of resin from V. oleifera in two experimental models of gastric ulcer oxidative-stress dependent.Materials and methods: Plant material was collected and the resin was subjected to partitioning with organic solvents. The buthanol fraction was subjected to chromatographic and spectrometric methods for isolation and structural elucidation. The resin was quantified for polyphenols and flavonoids by colorimetric methods. Furthermore, the antioxidant activity of resin was determined by three different methods. The ulcers were induced acutely in Swiss male mice with ethanol/HCl and indomethacin using single-doses of 10 and 100 mg/kg. The gastroprotection of the experimental groups was comparable to reference control lansoprazole (3 mg/kg).Results: The high content of polyphenols (∼82%) and the presence of epicatechin and eriodictyol were determined. The LD50 was estimated at 2500 mg/kg. At minimum (10 mg/kg) and maximum (100 mg/kg) dosage of resin, both in ethanol/HCl as indomethacin ulcer induction models demonstrate reduction of lesions (minimum: ∼97% and ∼66%; maximum: ∼95% and ∼59%).Discussion: The gastroprotection might be related to tannins, phenolic acids and flavonoids present in the resin by antioxidant properties.Conclusions: The results indicate that this resin has gastroprotective activity probably associated with the presence of phenolic antioxidant substances.

Pharmacological Extracts and Molecules from Virola Species: Traditional Uses, Phytochemistry, and Biological Activity

“…V. venosa is found in the Colombian Amazon region [ 14 ] and Brazil [ 15 ]. V. oleifera grows in the Atlantic Forest [ 16 ]; V. sebifera , in Honduras, Nicaragua, Costa Rica, and Panama [ 3 ]; and V. surinamensis is distributed from Costa Rica to the Amazon basin [ 17 ].…”

“…The oil extracted from the seeds of V. oleifera is popularly used for rheumatic pain, bronchial asthma, tumors in the joints, intestinal worms, halitosis, hemorrhoids, and skin diseases [ 9 ]. Validating its traditional use, V. oleifera resin ( Figure 1 ) demonstrated gastroprotective effects (reduced gastric mucosal damage) in an ethanol/HCl and indomethacin ulcer-induction mouse model at 10 and 100 mg/kg when administered orally 30 min before gastric lesion induction, comparable to the reference control lansoprazole (3 mg/kg) [ 16 ]. Moreover, the chronic administration of antioxidant resin from V. oleifera (50 mg/kg administered orally) also attenuates atherogenesis in the atherosclerotic LDLr −/− mouse model, through a decrease in vascular lipid deposition, the protection of the vascular endothelium and smooth muscle cells against peroxide-induced cytotoxicity, and the reduction of LPS-induced nitric oxide production in macrophages.…”

AKTIVITAS ANTIOKSIDAN FRAKSI DARI DAUN Garcinia kydia Roxburgh

“…Dimana Abs1 adalah absorbansi larutan FRAP setelah penambahan sampel uji dan Abs0 adalah absorbansi blanko (25).…”

Antioxidant Activity of Fractions from Garcinia hombroniana Pierre Leaves Extracts

“…11 The sample solution was piped 30μL, inserted into a microplate well, followed by additional of 270μL FRAP reagent, then incubated and the absorbance was measured. A mixture of 30μL methanol and 270μL FRAP reagents was used as blank.…”