EGR2 is a gonadotropin-induced survival factor that controls the expression of IER3 in ovarian granulosa cells

Jin, Hanyong; Won, Miae; Shin, Eunkyoung; Kim, Hong-Man; Lee, Kangseok; Bae, Jang–Ho

doi:10.1016/j.bbrc.2016.11.127

Cited by 11 publications

(7 citation statements)

References 37 publications

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“…Moreover EGR2 has been suggested to participate in multiple extracellular signals, including adipogenesis and carcinogenesis (50) and it was shown to control adipocyte differentiation, which potentially links to insulin resistance (51). in ovary, eGr2 was investigated, as an intra-ovarian factor, part of a novel signaling axis comprising of gonadotropins-eGr2-ier3, regulating the survival of granulosa cells during folliculogenesis in rats and humans (52). it was also found in the article above, to regulate the expression of myeloid cell leukemia 1 (Mcl-1), which belongs to the Bcl-2 family of proteins regulating cell survival.…”

Section: Discussionmentioning

confidence: 99%

New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation

et al. 2020

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oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well-known, its exact molecular mechanisms remain widely undiscovered. oocyte growth, differentiation and maturation are therefore the subject of scientific debate. Precious literature has indicated that the oocyte itself serves a regulatory role in the mechanisms underlying these processes. Hence, the present study performed expression microarrays to analyze the complete transcriptome of porcine oocytes during their in vitro maturation (iVM). Pig material was used for experimentation, as it possesses similarities to the reproductive processes and general genetic proximities of Sus scrofa to human. oocytes, isolated from the ovaries of slaughtered animals were assessed via the Brilliant cresyl Blue test and directed to iVM. a number of oocytes were left to be analyzed as the 'before iVM' group. oocyte mrna was isolated and used for microarray analysis, which was subsequently validated via rT-qPcr. The current study particularly focused on genes belonging to 'positive regulation of transcription, dna-dependent', 'positive regulation of gene expression', 'positive regulation of macromolecule metabolic process' and 'positive regulation of transcription from rna polymerase ii promoter' ontologies. FOS, VEGFA,

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Section: Discussionmentioning

confidence: 99%

New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation

et al. 2020

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“…Coincidentally, a number of zinc finger protein genes have clearly bilateral function in cancer and adipogenesis, such as ZNF395 , Zfp423 , Zfp521 , SLUG , Egr2 , FBI-1 , MCP-1 , YY1 , and so on. [ 4 , 6 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. However, this remains unknown for cases related to adipogenesis function, or the mechanism of Zfp217 .…”

Section: Introductionmentioning

confidence: 99%

The Emerging Role of Zfp217 in Adipogenesis

Xiang

Zhong

Peng

et al. 2017

IJMS

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Zinc finger protein 217 (Zfp217), a member of the krüppel-type zinc finger protein family, plays diverse roles in cell differentiation and development of mammals. Despite extensive research on the functions of Zfp217 in cancer, pluripotency and reprogramming, its physiological roles in adipogenesis remain unknown. Our previous RNA sequencing data suggest the involvement of Zfp217 in adipogenesis. In this study, the potential function of Zfp217 in adipogenesis was investigated through bioinformatics analysis and a series of experiments. The expression of Zfp217 was found to be gradually upregulated during the adipogenic differentiation in C3H10T1/2 cells, which was consistent with that of the adipogenic marker gene Pparg2. Furthermore, there was a positive, significant relationship between Zfp217 expression and adipocyte differentiation. It was also observed that Zfp217 could not only trigger proliferative defect in C3H10T1/2 cells, but also interact with Ezh2 and suppress the downstream target genes of Ezh2. Besides, three microRNAs (miR-503-5p, miR-135a-5p and miR-19a-3p) which target Zfp217 were found to suppress the process of adipogenesis. This is the first report showing that Zfp217 has the capacity to regulate adipogenesis.

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“…Furthermore, EGR2 deficient mice did not express other genes that are associated with myelin [21]. Jin et al have hypothesized that EGR2 is crucial for granulosa cells, as it interacts with gonadotropins to during folliculogenesis [22]. However, after IVM occurs, the expression of the gene has perhaps a less necessary function in the oocytes, which is supported by Brązert et al's study [23].…”

Section: Discussionmentioning

confidence: 87%

Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro – an oocyte model approach

Moncrieff

Kocherova

Bryja

et al. 2020

Medical Journal of Cell Biology

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The growth and development of the oocyte is essential for the ovarian follicle. Cumulus cells (CCs) - a population of granulosa cells - exchange metabolites, proteins and oocyte-derived paracrine factors with the oocyte through gap junctions, to contribute to the competency and health of the oocyte. This bi-directional communication of the cumulus-oocyte complex could be better understood through the micro-analysis of a porcine oocyte gene expression before in vitro maturation (IVM) and after. Additionally, the study of the somatic and gamete cells differentiation capability into neuronal lineage would be promising for future stem cell research as granulosa cells are easily accessible waste material from in vitro fertilization (IVF) procedures. Therefore, in this study, the oocytes of 45 pubertal Landrace gilts were isolated and the protein expression of the COCs were analyzed through micro-analysis techniques. Genes belonging to two ontological groups: neuron differentiation and negative regulation of cell differentiation have been identified which have roles in proliferation, migration and differentiation. Twenty identified porcine oocyte genes (VEGFA, BTG2, MCOLN3, EGR2, TGFBR3, GJA1, FST, CTNNA2, RTN4, MDGA1, KIT, RYK, NOTCH2, RORA, SMAD4, ITGB1, SEMA5A, SMARCA1, WWTR1 and APP) were found to be down-regulated after the transition of IVM compared to in vitro. These results could be applied as gene markers for the proliferation, migration and differentiation occurring in the bi-directional communication between the oocyte and CCs.Running title: Differentiation and neurogenesis in oocyte cells

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EGR2 is a gonadotropin-induced survival factor that controls the expression of IER3 in ovarian granulosa cells

Cited by 11 publications

References 37 publications

New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation

New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation

The Emerging Role of Zfp217 in Adipogenesis

Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro – an oocyte model approach

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