New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation

Abstract

oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well-known, its exact molecular mechanisms remain widely undiscovered. oocyte growth, differentiation and maturation are therefore the subject of scientific debate. Precious literature has indicated that the oocyte itself serves a regulatory role in the mechanisms underlying these processes. Hence, the present study performed expression microarrays to analyze the complete transcriptome of porcine oocytes during their in vitro maturation (iVM). Pig material was used for experimentation, as it possesses similarities to the reproductive processes and general genetic proximities of Sus scrofa to human. oocytes, isolated from the ovaries of slaughtered animals were assessed via the Brilliant cresyl Blue test and directed to iVM. a number of oocytes were left to be analyzed as the 'before iVM' group. oocyte mrna was isolated and used for microarray analysis, which was subsequently validated via rT-qPcr. The current study particularly focused on genes belonging to 'positive regulation of transcription, dna-dependent', 'positive regulation of gene expression', 'positive regulation of macromolecule metabolic process' and 'positive regulation of transcription from rna polymerase ii promoter' ontologies. FOS, VEGFA,

Pervasive 3′-UTR Isoform Switches During Mouse Oocyte Maturation

“…By contrast, the genes with downregulated transcripts or downregulated genes were enriched in functions related to ribosome biogenesis and translation ( Figure 2D and Supplementary Figure S2F ), again consistent with previous observations ( Zhao et al, 2020 ). Intriguingly, the 312 genes unidentifiable by DEG analysis but with DETs were functionally related to macromolecule metabolic process and regulation of gene expression ( Figure 2E ), coinciding with a recent study on porcine oocytes in vitro maturation where the authors found such genes were downregulated during maturation ( Brazert et al, 2020 ). Organelle organization was another enriched GO term for the 312 genes ( Figure 2E ), and the role of oocyte organelle has been shown to related to oocyte developmental competence and therefore oocyte quality ( Reader et al, 2017 ).…”

Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro – an oocyte model approach

“…Jin et al have hypothesized that EGR2 is crucial for granulosa cells, as it interacts with gonadotropins to during folliculogenesis [22]. However, after IVM occurs, the expression of the gene has perhaps a less necessary function in the oocytes, which is supported by Brązert et al's study [23].…”

Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes

“…It might relate to the embryo transport to the uterine cavity or to extrauterine pregnancy development. In addition, VEGF is considered to be a main mediator of angiogenesis in the primate ovulatory follicle in the ovary [ 34 ], and it may be a new marker of the porcine-oocyte-maturation competence during in vitro culture [ 35 ]. The balance of VEGF isoforms controls follicle progression and luteogenesis.…”