Regulation of endothelial barrier function by p120-catenin∙VE-cadherin interaction

Garrett, Joshua P; Lowery, Anthony M.; Adam, Alejandro P.; Kowalczyk, Andrew P.; Vincent, Peter

doi:10.1091/mbc.e16-08-0616

Cited by 32 publications

(37 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results indicate that the loss of the expression of α6 integrins from established tubes results in the loss of laminin-511, but not laminin-411, and that the loss of LM-511 and α6 integrins correlates with the loss of tube integrity. VE-cadherin is known to regulate endothelial barrier function (Garrett et al, 2017), and previous studies indicate that LM-511 and β1 integrins promotes the recruitment of VE-cadherin to cell-cell junctions to promote vessel stability (Song et al, 2017;Yamamoto et al, 2015). In preliminary studies, we did not detect obvious changes in the expression of VE-cadherin in endothelial cells depleted of α6 integrins; however, it seems likely possible that the loss of α6 integrins and laminin-511 perturbs cell-cell adhesion.…”

Section: Discussionmentioning

confidence: 99%

Laminin-511 and α6 Integrins Regulate the Expression of CXCR4 to Promote Endothelial Morphogenesis

Pumiglia

LaFlamme

2019

Preprint

View full text Add to dashboard Cite

AbstractDuring angiogenesis, endothelial cells engage components of the extracellular matrix through integrin-mediated adhesion. Endothelial expression of laminin-411 and laminin-511 are known to promote vessel stability. However, little is known about the contribution of these laminins to endothelial morphogenesis. We used two organotypic cell culture angiogenesis assays in conjunction with RNAi approaches to demonstrate that depletion of either the α4 chain of laminin-411 or the α5 chain of laminin-511 from endothelial cells inhibits sprouting and tube formation. Depletion of α6 integrins resulted in similar phenotypes. Gene expression analysis indicated that loss of either laminin-511 or α6 integrins inhibited the expression of CXCR4, a gene previously associated with angiogenic endothelial cells. Pharmacological or RNAi-dependent inhibition of CXCR4 suppressed endothelial sprouting and morphogenesis. Importantly, expression of recombinant CXCR4 rescued endothelial morphogenesis when the α6 integrin expression was inhibited. Additionally, the depletion of α6 integrins from established tubes resulted in the loss of tube integrity and laminin-511. Taken together, our results indicate that α6 integrins and laminin-511 can promote endothelial morphogenesis by regulating the expression of CXCR4 and suggest that the α6-dependent deposition of laminin-511 protects the integrity of established endothelial tubes.Summary statementEndothelial-secreted laminin-511 and α6 integrins promote endothelial morphogenesis by regulating the expression of the chemokine receptor, CXCR4. The depletion of α6 integrins from established tubes results in the loss of tube integrity and laminin-511.

show abstract

Section: Discussionmentioning

confidence: 99%

Laminin-511 and α6 Integrins Regulate the Expression of CXCR4 to Promote Endothelial Morphogenesis

Pumiglia

LaFlamme

2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Electrical cell-substrate impedance sensing (ECIS) technology can be applied to the analysis of cell migration, attachment and spreading, transepithelial electrical resistance, and proliferation. (1)(2)(3)(4)(5)(6)(7)(8)(9)(10) An electrical cell-substrate impedance sensor is one of the electrical biosensors that perform biochemical analysis entirely dependent on current and/or voltage measurements. (11,12) Using the impedance sensor, we can observe the cytotoxicity of a variety of toxicants and the status of stem and cancer cells.…”

Section: Introductionmentioning

confidence: 99%

“…This can be accomplished using a dedicated ECIS system or a data processing and visualization application. (8,9,17,24,25) For portable devices, a personal computer (PC) may be connected to the impedance measuring device. (21) The control of the graphical user interface (GUI) requires input devices such as a mouse and keyboard as well as a display device such as an LCD monitor.…”

Section: Introductionmentioning

confidence: 99%

User-friendly Interface for Electrical Cell-substrate Impedance Spectroscopy System

Kim¹,

Park²,

Park³

et al. 2019

Sensors and Materials

View full text Add to dashboard Cite

We propose an electrical cell-substrate impedance sensing (ECIS) system consisting of a low-cost compact analog front end (AFE) and a web-based user interface. Adopting a latemodel oscilloscope reduces the cost and size of the AFE. The web server was installed on an embedded computer to control the AFE and user interactions. The proposed system frees up the space normally needed for a display and keyboard/mouse in existing systems, which is an advantage in crowded laboratories, and it also prevents malfunctions due to inadvertent user input. In addition, the system control and experimental status can be monitored via smart devices such as smartphones and tablets anytime and anywhere. The accuracy and precision of the impedance measurement, which is the basic function of the ECIS system, are verified through experiments. Biomedical engineers using the proposed system report benefits due to the saving of laboratory space, the reduced probability of malfunction, the potential to monitor the experiment anytime and anywhere, and the ability to share experimental results with remote colleagues.

show abstract

“…When cells are attached and spread to the electrodes, the current is physically disturbed and the impedance increases; (1)(2)(3) this increase is used to assess cell behavior, response to drugs, and the barrier function of cancer or stem cells, because cell parameters such as cell status, cell number, and cell viability can be determined. (4)(5)(6)(7)(8)(9)(10)(11) These cell studies using ECIS ultimately aim to improve the quality of life. For example, cytotoxicity studies can examine the health effects of workers handling toxic substances.…”

Section: Introductionmentioning

confidence: 99%

“…(4) ECIS is utilized to investigate the regulation of endothelial permeability, whose malfunction leads to systemic vascular leakage resulting in edema and a loss of organ function. (5,6) It has also been applied to studies on cancer progress (7,11) and on the relationship between mesenchymal stem cells and oxygen tension in mesenchymal stem cell therapy. (10) The latter has great potential for human pathology, including cardiovascular disease, neurodegenerative disease, and osteoarthritis.…”

Section: Introductionmentioning

confidence: 99%

Internet-of-Things-based Electric Cell-substrate Impedance Sensing System

Park¹,

Lee²,

Kim³

et al. 2018

Sensors and Materials

View full text Add to dashboard Cite

In this paper, we propose an electric cell-substrate impedance sensing system that can be accessed from the internet. The hardware specification was derived using numerical simulations and culture chamber impedance emulators, and the impedance calculation methods were compared. In addition, we propose server-type software to easily control and monitor the system from the internet. Experimental results show that it is advantageous to use an acquisition device of 50 mega samples per second or more and to calculate the impedance using a sine fitting method rather than the lock-in detection method that is generally used. During experiments, there was no problem accessing the proposed server from the outside with standard computing devices.

show abstract

Regulation of endothelial barrier function by p120-catenin∙VE-cadherin interaction

Abstract: Maintaining VE-cadherin levels by inhibiting its endocytosis through p120-catenin binding is not sufficient for forming a restrictive barrier. Instead, p120-catenin binding to VE-cadherin is required to allow tyrosine-phosphorylated VE-cadherin to contribute to barrier formation.

Cited by 32 publications

References 40 publications

Laminin-511 and α6 Integrins Regulate the Expression of CXCR4 to Promote Endothelial Morphogenesis

Laminin-511 and α6 Integrins Regulate the Expression of CXCR4 to Promote Endothelial Morphogenesis

User-friendly Interface for Electrical Cell-substrate Impedance Spectroscopy System

Internet-of-Things-based Electric Cell-substrate Impedance Sensing System

Contact Info

Product

Resources

About